Characterization of industrial effluent samples and evaluation of heavy metal removal by Pseudomonas plecoglossicida and Serratia marcescens isolated from extreme environmental conditions
Paper Details
Characterization of industrial effluent samples and evaluation of heavy metal removal by Pseudomonas plecoglossicida and Serratia marcescens isolated from extreme environmental conditions
Abstract
Industrial wastewater contains metals that require effective removal before discharge, making bacterial treatment a relevant approach for investigation. This study characterized effluents from rice mill and automobile industries in Tiruchirappalli and food processing and dyeing industries in Coimbatore, Tamil Nadu, India, and evaluated metal removal by two bacterial isolates. Physicochemical parameters were analysed using standard procedures, and chromium, copper and zinc concentrations were measured by atomic absorption spectrometry. The isolates, identified through partial 16S rRNA gene sequencing as Pseudomonas plecoglossicida KMJ01 (C1) and Serratia marcescens KMJ03 (C2), were applied separately to each effluent at 5% (v/v) and incubated for 24 h at 150 rpm. Effluent pH ranged from 6.3 to 10.6, total solids from 350 to 4,450 mg/L, and chemical oxygen demand from 840 to 1,760 mg/L. Zinc removal ranged from 94.3% to 97.5% with C1 and from 89.6% to 98.5% with C2. C1 achieved 98.9% chromium removal in food processing effluent, while C2 achieved 97.8% in automobile effluent. Both isolates removed 99.3% of copper from rice mill effluent. In dyeing effluent, copper removal differed markedly, reaching 96.8% with C1 and 25.9% with C2. These findings identify both isolates as candidates for further evaluation, with performance depending on the metal and wastewater matrix. Controlled, replicated studies are needed to establish removal mechanisms and practical treatment feasibility.
Abonyi MN, Obi CC, Nwabanne JT, Aniagor CO. 2024. Emerging and ecofriendly biological methods for agricultural wastewater treatment. Environmental Systems Research 13(1), 45. DOI: 10.1186/s40068-024-00373-4.
Ahmed MJK, Ahmaruzzaman M. 2016. A review on potential usage of industrial waste materials for binding heavy metal ions from aqueous solutions. Journal of Water Process Engineering 10, 39–47. DOI: 10.1016/j.jwpe.2016.01.014.
Almazán-Sánchez PT, Linares-Hernández I, Solache-Ríos MJ, Martínez-Miranda V. 2016. Textile wastewater treatment using iron-modified clay and copper-modified carbon in batch and column systems. Water, Air, and Soil Pollution 227(4), 100. DOI: 10.1007/s11270-016-2801-7.
American Public Health Association, American Water Works Association, Water Environment Federation. 2005. Standard methods for the examination of water and wastewater. 21st edition. Washington, DC: American Public Health Association.
Arroyo-Herrera I, Román-Ponce B, Reséndiz-Martínez AL, Estrada-de Los Santos P, Wang ET, Vásquez-Murrieta MS. 2021. Heavy-metal resistance mechanisms developed by bacteria from Lerma–Chapala basin. Archives of Microbiology 203(4), 1807–1823. DOI: 10.1007/s00203-020-02140-2.
Aththanayake AMK, Deeyamulla MP, Megharaj M, Rathnayake IVN. 2025. Biofilm formation and detoxification of hexavalent chromium by Bacillus subtilis: A sustainable approach to bioremediation. Journal of Basic Microbiology 65(6), e70022. DOI: 10.1002/jobm.70022.
Ayangbenro AS, Babalola OO. 2017. A new strategy for heavy metal polluted environments: A review of microbial biosorbents. International Journal of Environmental Research and Public Health 14(1), 94. DOI: 10.3390/ijerph14010094.
Bhuvaneswari A, Asha B, Saranya T. 2015. Start up and enhancement granulation in an anaerobic baffled reactor for the treatment of textile wastewater. International Journal of Civil Engineering 4(5), 11–16.
Bilal M, Shah JA, Ashfaq T, Gardazi SMH, Tahir AA, Pervez A, Haroon H, Mahmood Q. 2013. Waste biomass adsorbents for copper removal from industrial wastewater—A review. Journal of Hazardous Materials 263, 322–333. DOI: 10.1016/j.jhazmat.2013.07.071.
Buscio V, Marín MJ, Crespi M, Gutiérrez-Bouzán C. 2015. Reuse of textile wastewater after homogenization–decantation treatment coupled to PVDF ultrafiltration membranes. Chemical Engineering Journal 265, 122–128. DOI: 10.1016/j.cej.2014.12.057.
Ceylan Ö, Ugur A. 2012. Bio-monitoring of heavy metal resistance in non-aeruginosa Pseudomonas and Pseudomonas related species. Journal of Biological and Environmental Sciences 6(18), 233–242.
Choi SB, Yun YS. 2004. Lead biosorption by waste biomass of Corynebacterium glutamicum generated from lysine fermentation process. Biotechnology Letters 26(4), 331–336. DOI: 10.1023/B:BILE.0000015453.20708.fc.
Choudhary M, Majumder S, Neogi S. 2015. Studies on the treatment of rice mill effluent by electrocoagulation. Separation Science and Technology 50(4), 505–511. DOI: 10.1080/01496395.2014.956225.
Das A. 2025. Geospatial techniques for the delineation of surface water potential zones and advanced optimization approaches for improving water quality assessment in the Mahanadi River Basin, Odisha, India. In: Gautam S, Joshi SK, Ambade B, editors. Blue sky, blue water: Strategies for protecting air and water quality in the 21st century. Cham: Springer Nature Switzerland, 437–455.
Fashola MO, Ngole-Jeme VM, Babalola OO. 2016. Heavy metal pollution from gold mines: Environmental effects and bacterial strategies for resistance. International Journal of Environmental Research and Public Health 13(11), 1047. DOI: 10.3390/ijerph13111047.
Fulke AB, Kotian A, Giripunje MD. 2020. Marine microbial response to heavy metals: Mechanism, implications and future prospect. Bulletin of Environmental Contamination and Toxicology 105(2), 182–197. DOI: 10.1007/s00128-020-02923-9.
Gabr RM, Hassan SHA, Shoreit AAM. 2008. Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a. International Biodeterioration and Biodegradation 62(2), 195–203. DOI: 10.1016/j.ibiod.2008.01.008.
Hansda A, Kumar V, Anshumali. 2016. A comparative review towards potential of microbial cells for heavy metal removal with emphasis on biosorption and bioaccumulation. World Journal of Microbiology and Biotechnology 32(10), 170.
Igiri BE, Okoduwa SIR, Idoko GO, Akabuogu EP, Adeyi AO, Ejiogu IK. 2018. Toxicity and bioremediation of heavy metals contaminated ecosystem from tannery wastewater: A review. Journal of Toxicology 2018, 2568038. DOI: 10.1155/2018/2568038.
Imron MF, Setiawan W, Putranto TWC, Abdullah SRS, Kurniawan SB. 2024. Biosorption of chromium by live and dead cells of Bacillus nitratireducens isolated from textile effluent. Chemosphere 359, 142389. DOI: 10.1016/j.chemosphere.2024.142389.
Javed S, Ali A, Alam S, Rafique M, Gul B, Chaudhary HJ, Santali EY. 2024. Biosorption of cadmium and chromium from wastewater using Bacillus xiamenensis and Bacillus cereus isolated from the sugarcane rhizosphere. ACS ESandT Water 4(9), 4140–4149. DOI: 10.1021/acsestwater.4c00406.
Jayaraman J, Kumaraswamy J, Rao YKSS, Karthick M, Baskar S, Anish M, Sharma A, Yadav AS, Alam T, Ammarullah MI. 2024. Wastewater treatment by algae-based membrane bioreactors: A review of the arrangement of a membrane reactor, physico-chemical properties, advantages and challenges. RSC Advances 14(47), 34769–34790. DOI: 10.1039/D4RA04417G.
Kanu I, Achi OK. 2011. Industrial effluents and their impact on water quality of receiving rivers in Nigeria. Journal of Applied Technology in Environmental Sanitation 1(1), 75–86.
Karichappan T, Venkatachalam S, Jeganathan PM, Sengodan K. 2013. Treatment of rice mill wastewater using continuous electrocoagulation technique: Optimization and modelling. Journal of the Korean Chemical Society 57(6), 761–768. DOI: 10.5012/jkcs.2013.57.6.761.
Kaur H, Sharma G. 2015. Removal of dyes from textile industry effluent: A review. Int J Humanit Soc Sci 2(4), 59–63.
Khan N, Hadeed MZ, Khan HA, Yaqoob H, Idrees M, Khan Z, Khan AA. 2025. Assessment of physicochemical characteristics of wastewater from selected vehicle service stations in Peshawar. Frontier in Medical and Health Research 3(8), 397–416. DOI: 10.5281/zenodo.17348690.
Khan WU, Ahmed S, Dhoble Y, Madhav S. 2023. A critical review of hazardous waste generation from textile industries and associated ecological impacts. Journal of the Indian Chemical Society 100(1), 100829. DOI: 10.1016/j.jics.2022.100829.
Marrero J, Díaz A, Valle A, Cantero D, Gómez JM, Coto O. 2009. Nickel and cobalt removal capacities of native metal-resistant bacteria. Advanced Materials Research 71–73, 617–620. DOI: 10.4028/www.scientific.net/AMR.71-73.617.
Matyar F, Akkan T, Uçak Y, Eraslan B. 2010. Aeromonas and Pseudomonas: Antibiotic and heavy metal resistance species from Iskenderun Bay, Turkey (northeast Mediterranean Sea). Environmental Monitoring and Assessment 167, 309–320. DOI: 10.1007/s10661-009-1051-1.
Metcalf and Eddy, AECOM. 2014. Wastewater engineering: Treatment and resource recovery. 5th edition. New York: McGraw-Hill Education.
Mustapha LS, Obayomi KS. 2025. Parametric optimization of aloe vera coagulant for nitrate removal from textile wastewater using response surface methodology (RSM). Results in Chemistry 14, 102111. DOI: 10.1016/j.rechem.2025.102111.
Nanni M. 2016. Water challenges in the IGAD region: Towards new legal frameworks for cooperation. Water International 41(4), 635–651. DOI: 10.1080/02508060.2016.1169620.
Nur-E-Alam M, Mia MAS, Ahmad F, Rahman MM. 2020. An overview of chromium removal techniques from tannery effluent. Applied Water Science 10(9), 205. DOI: 10.1007/s13201-020-01286-0.
Pradhan A, Sahu SK. 2004. Process details and effluent characteristics of a rice mill in the Sambalpur district of Orissa. Journal of Industrial Pollution Control 20(1), 111–124.
Pradhan D, Sukla LB, Sawyer M, Rahman PKSM. 2017. Recent bioreduction of hexavalent chromium in wastewater treatment: A review. Journal of Industrial and Engineering Chemistry 55, 1–20. DOI: 10.1016/j.jiec.2017.06.040.
Punzi M, Nilsson F, Anbalagan A, Svensson BM, Jönsson K, Mattiasson B, Jonstrup M. 2015. Combined anaerobic–ozonation process for treatment of textile wastewater: Removal of acute toxicity and mutagenicity. Journal of Hazardous Materials 292, 52–60. DOI: 10.1016/j.jhazmat.2015.03.018.
Quintelas C, Rocha Z, Silva B, Fonseca B, Figueiredo H, Tavares T. 2009. Biosorptive performance of an Escherichia coli biofilm supported on zeolite NaY for the removal of Cr(VI), Cd(II), Fe(III) and Ni(II). Chemical Engineering Journal 152(1), 110–115. DOI: 10.1016/j.cej.2009.03.039.
Rizvi A, Ahmed B, Zaidi A, Khan MS. 2020. Biosorption of heavy metals by dry biomass of metal tolerant bacterial biosorbents: An efficient metal clean-up strategy. Environmental Monitoring and Assessment 192(12), 801. DOI: 10.1007/s10661-020-08758-5.
Roy R, Samanta S, Pandit S, Naaz T, Banerjee S, Rawat JM, Chaubey KK, Saha RP. 2024. An overview of bacteria-mediated heavy metal bioremediation strategies. Applied Biochemistry and Biotechnology 196(3), 1712–1751. DOI: 10.1007/s12010-023-04614-7.
Saravanan A, Kumar PS, Jeevanantham S, Karishma S, Tajsabreen B, Yaashikaa PR, Reshma B. 2021. Effective water/wastewater treatment methodologies for toxic pollutants removal: Processes and applications towards sustainable development. Chemosphere 280, 130595. DOI: 10.1016/j.chemosphere.2021.130595.
Sghaier I, Guembri M, Chouchane H, Mosbah A, Ouzari HI, Jaouani A, Cherif A, Neifar M. 2019. Recent advances in textile wastewater treatment using microbial consortia. Journal of Textile Engineering and Fashion Technology 5(3), 134–146. DOI: 10.15406/jteft.2019.05.00194.
Sharma P, Iqbal HMN, Chandra R. 2022. Evaluation of pollution parameters and toxic elements in wastewater of pulp and paper industries in India: A case study. Case Studies in Chemical and Environmental Engineering 5, 100163. DOI: 10.1016/j.cscee.2021.100163.
Shrestha R, Ban S, Devkota S, Sharma S, Joshi R, Tiwari AP, Kim HY, Joshi MK. 2021. Technological trends in heavy metals removal from industrial wastewater: A review. Journal of Environmental Chemical Engineering 9(4), 105688. DOI: 10.1016/j.jece.2021.105688.
Sibi G. 2019. Factors influencing heavy metal removal by microalgae—A review. Journal of Critical Reviews 6(6), 29–32. DOI: 10.22159/jcr.2019v6i6.35600.
Sonkar M, Nag K, Mahapatra K, Chandra V, Sankhyan S, Ray S, Kumar V, Kumar P, Upadhyay TK, Kumar S, Kumar V. 2026. Recent advances in bacterial extracellular polymeric substances mediated heavy metal removal: An eco-friendly and innovative approach. Bioremediation Journal 30(2), 266–289. DOI: 10.1080/10889868.2024.2440752.
Sreedevi PR, Suresh K, Jiang G. 2022. Bacterial bioremediation of heavy metals in wastewater: A review of processes and applications. Journal of Water Process Engineering 48, 102884. DOI: 10.1016/j.jwpe.2022.102884.
Sudhir S, Prasanna K. 2023. Treatment of wastewater generated from automobile service stations using corn cob as adsorbent. RASĀYAN Journal of Chemistry 16(1), 207–213. DOI: 10.31788/RJC.2023.1618041.
Tessema RS, Tarekegn MM, Worku MA, Engida AN, Van Griensven A. 2024. Performance evaluation of integrated Upflow Anaerobic Sludge Blanket reactor with trickling filter used for municipal wastewater treatment and effluent reuse potential for agriculture. Environmental Systems Research 13(1), 39. DOI: 10.1186/s40068-024-00353-8.
Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22(22), 4673–4680. DOI: 10.1093/nar/22.22.4673.
Thorat BN, Sonwani RK. 2022. Current technologies and future perspectives for the treatment of complex petroleum refinery wastewater: A review. Bioresource Technology 355, 127263. DOI: 10.1016/j.biortech.2022.127263.
Tomei MC, Pascual JS, Angelucci DM. 2016. Analysing performance of real textile wastewater bio-decolourization under different reaction environments. Journal of Cleaner Production 129, 468–477. DOI: 10.1016/j.jclepro.2016.04.028.
Tripathi S, Arora N, Gupta P, Pruthi PA, Poluri KM, Pruthi V. 2019. Microalgae: An emerging source for mitigation of heavy metals and their potential implications for biodiesel production. In: Advanced biofuels. Woodhead Publishing, 97–128. DOI: 10.1016/B978-0-08-102791-2.00004-0.
Tsekova K, Todorova D, Dencheva V, Ganeva S. 2010. Biosorption of copper(II) and cadmium(II) from aqueous solutions by free and immobilized biomass of Aspergillus niger. Bioresource Technology 101(6), 1727–1731. DOI: 10.1016/j.biortech.2009.10.012.
Vocciante M, Finocchi A, De Folly D’Auris A, Conte A, Tonziello J, Pola A, Reverberi AP. 2019. Enhanced oil spill remediation by adsorption with interlinked multilayered graphene. Materials 12(14), 2231. DOI: 10.3390/ma12142231.
Volarić A, Svirčev Z, Tamindžija D, Radnović D. 2021. Microbial bioremediation of heavy metals. Hemijska Industrija 75(2), 103–115.
Wang H, Wang T, Zhang B, Li F, Toure B, Omosa IB, Chiramba T, Abdel-Monem M, Pradhan M. 2014. Water and wastewater treatment in Africa—Current practices and challenges. CLEAN–Soil, Air, Water 42(8), 1029–1035.
Wang R, Shu J, Chen M, Wang R, He D, Wang J, Tang C, Han Y, Luo Z. 2021. An innovative method for fractionally removing high concentrations of Ni²⁺, PO₄³⁻, TP, COD, and NH₄⁺-N from printed-circuit-board nickel plating wastewater. Separation and Purification Technology 260, 118241. DOI: 10.1016/j.seppur.2020.118241.
Wei W, Ma R, Sun Z, Zhou A, Bu J, Long X, Liu Y. 2018. Effects of mining activities on the release of heavy metals (HMs) in a typical mountain headwater region, the Qinghai-Tibet Plateau in China. International Journal of Environmental Research and Public Health 15(9), 1987. DOI: 10.3390/ijerph15091987.
Xu S, Xing Y, Liu S, Hao X, Chen W, Huang Q. 2020. Characterization of Cd²⁺ biosorption by Pseudomonas sp. strain 375, a novel biosorbent isolated from soil polluted with heavy metals in Southern China. Chemosphere 240, 124893. DOI: 10.1016/j.chemosphere.2019.124893.
Yaseen DA, Scholz M. 2019. Textile dye wastewater characteristics and constituents of synthetic effluents: A critical review. International Journal of Environmental Science and Technology 16(2), 1193–1226. DOI: 10.1007/s13762-018-2130-z.
Zeng GM, Zhao MH, Huang DL, Lai C, Huang C, Wei Z, Xu P, Li NJ, Zhang C, Li FL, Cheng M. 2013. Purification and biochemical characterization of two extracellular peroxidases from Phanerochaete chrysosporium responsible for lignin biodegradation. International Biodeterioration and Biodegradation 85, 166–172.
Zhang F, Yan X, Zeng C, Zhang M, Shrestha S, Devkota LP, Yao T. 2012. Influence of traffic activity on heavy metal concentrations of roadside farmland soil in mountainous areas. International Journal of Environmental Research and Public Health 9(5), 1715–1731. DOI: 10.3390/ijerph9051715.
Zhao M, Zhang C, Zeng G, Huang D, Xu P, Cheng M. 2015. Growth, metabolism of Phanerochaete chrysosporium and route of lignin degradation in response to cadmium stress in solid-state fermentation. Chemosphere 138, 560–567. DOI: 10.1016/j.chemosphere.2015.07.019.
Jeyadevi Kadarkarai, Daffodil Achsah Miraclyn Selvaprasad, Vasanthy Muthunarayanan*, 2026. Characterization of industrial effluent samples and evaluation of heavy metal removal by Pseudomonas plecoglossicida and Serratia marcescens isolated from extreme environmental conditions. Int. J. Biosci., 29(2), 140-155.
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