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Characterization of Lasiodiplodia pseudotheobromae associated with citrus stem-end rot disease in Bangladesh

Razia Sultana, Md. Samiul Islam, Hafizur Rahman, Md. Shah Alam, Md. Asadul Islam, Biswanath Sikdar

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Int. J. Biosci.13(5), 252-262, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.252-262


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Stem-end rot is the most common postharvest citrus fruit diseases in Bangladesh and worldwide. The aim of the present study was to identify the causal pathogen and assays to in vitro control of the pathogen against different plant extracts. The pathogen was isolated from infected fruit on PDA media forming white to grey colonies. The maximum mycelial growth of isolate was recorded at temperature 250C and 300C with radial growth 88.00 mm and 79.00 mm respectively on PDA media. Different pH, carbohydrates, and salts were also used to lead the growth profiling which also revealed the morphology of isolated pathogen. The isolated fungus was identified based on a combination of morphological characteristics and sequencing of the internal transcribed spacers (ITS) region and the gene encoding lp1 small subunit ribosomal RNA gene. The nucleotide sequence comparison and phylogenetic analyses indicate approximately 99% identity with the fungal pathogen Lasiodiplodia pseudotheobromae. Based on the morphological characteristics, molecular and phylogenetic approaches, the causal fungus was identified as L. pseudotheobromae. Different plants extract were used to evaluate in vitro control of the citrus fruit end-rot disease pathogen. The methanolic extract of Azadirachta indica and Psidium guajava significantly inhibited the mycelial growth (93.78% and 90.90% respectively) of L. pseudotheobromae in vitro.


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Characterization of Lasiodiplodia pseudotheobromae associated with citrus stem-end rot disease in Bangladesh

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