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Characterization of seed storage proteins of different varieties of Brassica napus seeds

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Research Paper 01/11/2013
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Characterization of seed storage proteins of different varieties of Brassica napus seeds

Fazal Akbar, Arifa Farooqi, Tahir Naeem Khan, Muhamamd Suleman, Syed Shujait Ali, Ishaq Khan, Shahid Ali
Int. J. Biosci.3( 11), 164-171, November 2013.
Certificate: IJB 2013 [Generate Certificate]
Key: Cluster analysisElectrophorogramGenetic diversityPhysiochemical parametersSDS-PAGE

Abstract

Brassica napus (Canola), an important cash crop in Pakistan, has rich oil (40%) and protein (15%) content. The present study was designed to investigate nine different varieties of B. napus (Tarch, Altex, Dasi, Dunkled, V-248, Bulb 98, Rainbow, Asker and Habib-98), for various physiochemical parameters (moisture content, thousand seed weight [TSW] and electrical conductivity [EC]), and also to investigate the genetic relationships among these genotypes using Sodium dodecyl sulphate-polyacralamide gel electrophoresis (SDS-PAGE). The maximum amount of moisture content was 6.31% for variety Dasi and minimum of 4.26% for variety Dunkled. The maximum TSW value was found for V-248 (4.22 g) and the minimum value for Dunkled variety (2.11 g). Electrical conductivity for different varieties was estimated at time intervals of 10 min, 20, 30, 40, 50, 60 and 70 minutes. The EC was determined by conductometer and the mean value ranged from 3.0-6.5 µ sec. Seeds protein analyzed by SDS-PAGE showed a significant genetic variation among the nine different varieties of B. napus, and they were clustered into two distinct clades with significant genetic distances. Our results demonstrate that seed storage protein profiles could be useful marker in the studies of genetic diversity and classification of adopted cultivars, thereby improving the efficiency of Brassica breeding programs in the cultivar development.

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