Characterization of seed storage proteins of different varieties of Brassica napus seeds

Paper Details

Research Paper 01/11/2013
Views (509) Download (9)
current_issue_feature_image
publication_file

Characterization of seed storage proteins of different varieties of Brassica napus seeds

Fazal Akbar, Arifa Farooqi, Tahir Naeem Khan, Muhamamd Suleman, Syed Shujait Ali, Ishaq Khan, Shahid Ali
Int. J. Biosci.3( 11), 164-171, November 2013.
Certificate: IJB 2013 [Generate Certificate]

Abstract

Brassica napus (Canola), an important cash crop in Pakistan, has rich oil (40%) and protein (15%) content. The present study was designed to investigate nine different varieties of B. napus (Tarch, Altex, Dasi, Dunkled, V-248, Bulb 98, Rainbow, Asker and Habib-98), for various physiochemical parameters (moisture content, thousand seed weight [TSW] and electrical conductivity [EC]), and also to investigate the genetic relationships among these genotypes using Sodium dodecyl sulphate-polyacralamide gel electrophoresis (SDS-PAGE). The maximum amount of moisture content was 6.31% for variety Dasi and minimum of 4.26% for variety Dunkled. The maximum TSW value was found for V-248 (4.22 g) and the minimum value for Dunkled variety (2.11 g). Electrical conductivity for different varieties was estimated at time intervals of 10 min, 20, 30, 40, 50, 60 and 70 minutes. The EC was determined by conductometer and the mean value ranged from 3.0-6.5 µ sec. Seeds protein analyzed by SDS-PAGE showed a significant genetic variation among the nine different varieties of B. napus, and they were clustered into two distinct clades with significant genetic distances. Our results demonstrate that seed storage protein profiles could be useful marker in the studies of genetic diversity and classification of adopted cultivars, thereby improving the efficiency of Brassica breeding programs in the cultivar development.

VIEWS 1

Akbar M, Saleem U, Tahira MY, Nasim I. 2007. Utilization of genetic variability, correlation and path analysis for seed yield improvement in mustard, brassica juncea. Journal of Agricultural Research 45, 1.

Ali S, Munir I, Arif M, Inamullah, Farahatullah, Ali I, Iqbal A, Ahmad M, khan MW, Abbas J, Swati ZA. 2011. Characterization of B. napus germplasm based  on molecular markers. African Journal of Biotechnology 10, 3035-3039.

Ali W, Munir I, Ahmed MA, Muhammad W, Ahmad N, Durrishahwar, Ali S, Swati ZA. 2007. Molecular characterization of some local and exotic B. juncea germplasm. African Journal of Biotechnology 6, 1634-1638.

Bell JM, Rakow G, Downey RK. 2000. Comparison of amino acid and protein levels in oli-extracted seeds of Brassica and Sinapis species, with observations on environmental effects. Canadian Journal of Animal Sciences 80, 169-174.

Farahbakhsh H, Pakgohar N, Karimi A. 2006. Effects of nitrogen and sulfur fertilizers on yield, yield components and oil content of Oilseed Rape (Brassica napus L.). Asian Journal of Plant Sciences 5, 112-115.

Gladis T, Hammer KH. 1990. Die Gaterslebener Brassica-kollektion eine Einführung. Kulturpflanze 38,121-156.

Mailer RJ, Colton RT, Bree BL. 1998. Quality of Australian canola. Canola association of Australia. ISSN, 1322-9397.

Mukhlesur M, Hirata Y, Shah A. 2004. Genetic variation within Brassica rapa cultivars using SDS-PAGE for seed protein and isozyme analysis. Journal of Biological Sciences 4, 239-242.

Nei N, Lie W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of Natural Academy of Sciences 76, 5269-5273.

Prakash S, Hinata K. 1980. Taxonomy, cytogenetics and origin of crop Brassica, a review. Operational Botany 55, 1-57.

Rabbani MA, Iwabuchi A, Murakami Y, Suzuki T, Takayanagi K. 1998. Phenotypic variation and the relationships among mustard (Brassica juncea L.) germplasm from Pakistan. Euphytica 101, 357-366. http://dx.doi.org/10.1023/A:1018305201279

Rabbani MA, Qureshi AA, Afzal M, Anwar R, Komatsu S. 2001. Characterization of mustard Brassica juncea (l.) Czern. & coss germplasm by SDS-PAGE of total seed proteins. Pakistan Journal of Botany 33, 173-179.

Shuaib M, Zeb A, Ali Z, Ali W, Ahmad T, Khan I. 2007. Characterization of wheat varieties by seed storage protein electrophoresis. African Journal of Biotechechnology 6, 497-500.

Stephen H, Volkmar KM, Miller PR. 2001. Comparing canola, field pea, dry bean and durum wheat crops growing in saline media. Crop Sciences 41, 1827-1833. http://dx.doi.org/10.2135/cropsci2001.1827

Tankesley SD, Jones RA. 1986. Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Horticulture Sciences 16, 179-181.

UN. 1935. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japan Journal of Botany 7, 389-452.

Zia MA, Hadi F, Akbar H, Akbar F, Ullah Z, Khan I. 2008. Physiochemical and molecular analysis of Brassica napus seeds of different varieties. Asian Journal of Plant Sciences 7, 85-89.