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Characterization, pathogenecity and antibiogram study of Salmonella species isolated from apparently healthy and diarrhoeic calves

Research Paper | January 1, 2013

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Muhammad Mominul Islam, M. Ashrafuzzaman, Md. Hazrat Ali, Khair Ahmed Choudhury, Md. Shahidur Rahman Khan

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Int. J. Biosci.3( 1), 109-120, January 2013


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Salmonellosis in calves appears as one of the major problems in Bangladesh and causes substantial economic loss. The present study was conducted with a view to isolate and identify Salmonella species from apparently healthy and diarrhoeic calves of Char Nilokkhiya, BAU dairy farm and calves those came to Veterinary clinic. During the present study, out of 126 rectal swab samples, 10 samples were identified as positive for Salmonella. The isolates were named as D1, D2, D3, D4, D5, D6, H1, H2, H3 and H4. Of the 10 isolates 4 were isolated from 71 apparently healthy calves and 6 were isolated from 55 diarrhoeic calves. In case of apparently healthy individual, the percentage of positive samples from those areas was 6.25%, 4% and 6.66% respectively whereas in case of diarrhoeic calves it was 11.11%, 9.52% and 12% respectively. The overall prevalence of Salmonella in diarrhoeic calves (10.90%) was remarkably higher than in apparently healthy calves (5.633%). Pathogenicity test was performed using 5 groups of day old white mice each consisting of 4 animals by inoculating the isolates orally and intraperitonially which caused diarrhoea and death to the mice used. The group of animals was numbered as Group-A, Group-B, Group-C, Group-D and Goup-E. Two mice from each of Group-A and Group-B were inoculated orally each with o.5 ml. of the inoculum of the isolate No. H1 and H2 respectively, while the remaining two mice of the same groups were injected intraperitoneally each with 0.25 ml. of the inoculum of the same isolate. Similarly isolate no. D1 and D2 were inoculated into the animal group C and D respectively while Group-E was kept as control and inoculated with nutrient broth. The antimicrobial susceptibility of all the Salmonella isolates was analyzed by the disc diffusion method. Most of the isolates were highly sensitive to ciprofloxacin, gentamycin and spiramycin, moderately sensitive to amoxycillin, streptomycin and oxytetracycline and nearly resistant to penicillin and slulphamethoxazole.


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Characterization, pathogenecity and antibiogram study of Salmonella species isolated from apparently healthy and diarrhoeic calves

Aggarwal P, Sarkar R, Singh M, Grover BD, Anand BR, Raichowdhuri AN. 1983. Salmonella bareilly infection in a paediatric hospital of New Delhi. Indian Journal of Medical Research 78, 22-5.

Amin MM. 1969. Isolation and Identification of Salmonella from wild birds, rodents and insects. MS Thesis, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Banani M, Pourbakhsh SA, Khaki P and Nikookhesal GH. 2003. Serotyping and drug sensitivity of Salmonella isolates from commercial chickens and domestic pigeons submitted to Razi Institute. Pajouhesh-va-sadandegi-in-Animal Sciences 59, 92-96.

Barrington GM, Gay JM and Evermann JF. 2002. Biosecurity for neonatal gastrointestinal diseases. Veterinary Clinics of North America. Food Animal Practical 18(1), 7-34.

Blood DC, Henderson AJ, Radosintits AJH and Gay CC. 2003. A text book of the diseases of animals. 9th edition, 809-829.

Buxton A and Fraser G. 1977. Animal Microbiology. Vol. 1. Blackwell Scientific Publications, Oxford, London, Edinburg, Melbourne, 85-86.

Chugh TD and Suheir A. 1983. Drug resistance among Salmonellae in Kuwait. Tropical and Geographical Medicine 35(1), 37-41.

Dallal MM, Khorramizadeh MR and MoezArdalan K. 2006. Occurrence of enteropathogenic bacteria in children under 5 years with diarrhoea in south Tehran. Eastern Mediterranean Health Journal 12(6), 792-7.

Davison HC, Smith RP, Pascoe SJ, Sayers AR, Davies RH, Weaver JP, Kidd SA, Dalziel RW and Evans SJ. 2005. Prevalence, incidence and geographical distribution of serovars of Salmonella on dairy farms in England and Wales. Veterinary Record 157(22), 703-11.

Dorn CR, Silapanuntakul R, Angrick EJ, Shipman LD. 1992. Plasmid analysis and epidemiology of Salmonella enteritidis infection in three commercial layer flocks. Avian Diseases 36, 844-51.

Field HI. 1948. A survey of bovine salmonellosis in mid and west Wales. Veterinary Journal 104, 251– 339.

Freeman BA. 1985. Burrows textbook of microbiology. 22nd edition. W. B. Saunders Company, Philadelphia, London, Toronto, Mexici City, Rio de Janerio, Sydney, Tokyo, 464-472.

Gibson EA. 1965. Reviews of the progress of dairy science. Journal of Dairy Research 32, 17–134.

Gouws PA, Visser M, Brözel V. 1998. A polymerase chain reaction procedure for the detection of Salmonella spp. within 24 hours. Journal of Food Protection 61, 1039-1042.

Hollinger K, Wray C, Evans S, Pascoe S, Chapell S, Jones Y. 1998. Salmonella Typhimurium DT104 in cattle in Great Britain. Journal of the American Veterinary Medical Association 213, 1732–1733.

Hossain KM. 2002. Characterization of bacteria isolated from diarrhoeic calves. MS. Thesis, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Hughes LE, Gibson EA, Roberts HE, Davies ET, Davies G, Sojka WJ. 1971. Bovin salmonellosis in England and Wales. British Veterinary Journal 127, 225–238.

Jones TF, Ingram LA, Fullerton KE, Marcus R, Anderson BJ, McCarthy PV, Vugia D, Shiferaw B, Haubert N, Wedel S, Angulo FJ. 2006. A case-control study of the epidemiology of sporadic Salmonella infection in infants. Pediatrics 118(6), 2380-87.

Khan AA, Melvin CD, Dagdag EB. 2007. Identification and molecular characterization of Salmonella spp. from unpasteurized orange juices and identification of new serotype Salmonella strain S. enterica serovar Tempe. Food Microbiology 24(5), 539-43.

Kobayashi H, Kanazaki M, Shimizu Y, Nakajima H, Khatun MM, Hata E and Kubo M. 2007. Salmonella isolates from cloacal swabs and footpads of wild birds in the immediate environment of Tokyo Bay. Journal of Veterinary Medical Science 69(3), 309-11.

McDonough PL, Fogelman D, Shin SJ, Brunner MA, Lein DH. 1999. Salmonella enterica serotype Dublin infection: an emerging infectious disease for the northeastern United States. Journal of Clinical Microbiology 37, 2418–2427.

McEvoy JM, Doherty AM, Sheridan JJ, Blair IS and McDowell DA. 2003. The prevalence of Salmonella spp. in bovine faecal, rumen and carcass samples at a commercial abattoir. Journal of Applied Microbiology 94(4), 693-700.

Much P, Pichler J and Allerberger F. 2007. Food borne infectious outbreaks, Austria 2005. Wien Klin Wochenschr 119(5-6), 150-7.

Rahman MM. 1977. Isolation and identification of Salmonella and Escherichia coli from river water, pond water, tap water, tube well water and poultry feed. MS Thesis, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Rings DM. 1985. Salmonellosis in calves. Veterinary Clinics of North America. Food Animal Practical 1(3), 529-39.

Sato G, Furuta Y, Kodama H, Iwao T and Oka M. 1975. Enzootic occurrence of chloramphenicol-resistant Salmonella typhimurium var copenhagen in calf population. American Journal of Veterinary Research 36(6), 839-41.

Wray C, Sojka WJ. 1977. Reviews of the progress of dairy science: bovine salmonellosis. Journal of Dairy Research 44, 383–425.