Chemical compositions of peppermint (Mentha piperita L.) grown in Isfahan province, Iran

Paper Details

Research Paper 01/07/2015
Views (235) Download (5)

Chemical compositions of peppermint (Mentha piperita L.) grown in Isfahan province, Iran

Leila Fahmideh, Ali Sargazi, Ali Mehras Mehrabi, Arezoo Armin
J. Bio. Env. Sci.7( 1), 72-76, July 2015.
Certificate: JBES 2015 [Generate Certificate]


Peppermint is scientific named Mentha peperita L. belongs to Lamiaceae family. The Mediterranean region can be described as the center of the genus. Peppermint is one of most important medicinal plants that was used in food, cosmetics hygienic industries in most ofthe developed countries. Mentha species have strong anti-spasmodic, anti-sickness, anti-helminthic, carminative and stomachic activities. Breeding programs need to identify the best land races as base population. Existence sufficient genetic diversity also is important in selection the superior cultivars. Mentha piperita L. or peppermint oil is one of the most popular and widely used essential oils, mostly because of its main components menthol and menthone. This study was conducted to determine the major components of peppermint cultivated in Isfahan province. The aerial parts of plants were collected in Isfahan (Semirom) Province in 2014. The aerial parts of plants analyzed by using GC/MS in Islamic Azad University Isfahan (Khorasgan). Twenty five compounds were identified in aerial parts of peppermint. The results obtained in the study showed that major components of peppermint were Camphane (15.203%), Menthone (12.013%), Menthol (11.406%), β-Pinene (7.62%), Pulegone (6.42%), β-Cubebene (4.95%), α-Pinene (4.012%), γ-Terpinene (4.081%), Carane (3.82%) and Piperiton (3.05%). This land race was collected from Isfahan province having high amount of Camphane, Menthone and Menthol so is promising population for breeding programs via selection to improve medicinal value of this important medicinal plant.


Aflatuni A, Heikkinen K, Tomperi P, Jalonen J, Laine K. 2000. Variation in the extract composition of mints of different origin cultivated in Finland. Journal of Essential Oil Research 12, 462–466.

Ahmad I, Ahmad MSA, Hussain M, Hameed M, AshrafMY, Koukab MY. 2009. Spatio-temporal effects on species classification of medicinal plants in Soone Valley of Pakistan. InternationalJournal ofAgriculture and Biology 11, 64-68.

Arldogan BC, Baydaf H, Kaya S, Demirci M, Ozbaw D, Mumcu E. 2002. Antimicrobial Activity and Chemical Composition of Some essential oils. Archives of Pharmaceutical Research 25, 860-864.

Ashok K, Chandra S, Dutt P, Lal Kaul B, Tava A, Avato P. 1999. Essential oil composition of Mentha piperita L. from different environments of north India. Flavour and Fragrance Journal 14, 5-8.

Behnam S, Farzaneh M, Ahmadzadeh M, Tehrani AS. 2006. Composition and antifungal activity of essential oils of Mentha piperita and Lavendula angustifolia on post-harvest phytopa-thogens. Communication in Agriculture and Applied Biological Sciences 71, 1321-6.

Carla D, Decorti D. 2009. Ultrasound-assisted extraction coupled with under vacuum distillation of .avour compounds from spearmint (carvone-rich) plants: Comparison with conventional hydrodis-tillation. Ultrasonics Sonochemistry 16, 795–799.

Derwich E, Benziane Z, Taouil R, Senhaji O, Touzani M. 2010. Aromatic Plants of Morocco: GC/MS Analysis of the Essential Oils of Leaves of Mentha piperita. Advances in Environmental Biology 4, 80-85.

Eteghad S, Mirzaei H, Pour SF, Kahnamui S. 2009. Inhibitory Effects of Endemic Thymus vulgaris and Mentha piperita essential oils on Escherichia coli O157: H7. Research Journal of Biological Sciences 4, 340-344.

Iscan G, Kirimer N, Kurkcuoglu M, Baser KHC, Demirci F. 2002. Antimicrobial screening of Mentha piperita L. essential oils. Journal of Agriculture and Food Chemistry 50, 3943 – 46.

Jaimand K, Rezaii MB, Asgari F. 2001. Comparative of two distillation methods and plant growth stages on the essential oil content and composition of Mentha piperita L. Iranian Journal of Medicinal and Aromatic Plants 18, 11-21.

Ka MH, Choi EH, Chun HS, Lee KG. 2005. Antioxidative activity of volatile extracts isolated from Angelica tenuissimae roots, peppermint leaves, pine needles, and sweet flag leaves. Journal of Agriculture and Food Chemistry 53, 4124 – 9.

Mirza M, Ghoraishi F, Bahadori A. 2011. Effect of harvesting time on essential oils content and composition of Salvia officinalis L. and Mentha piperita L. in Khuzestan province. Iranian Journal of Medicinal and Aromatic Plants 26, 531-543.

Mozaffarian V. 1996. A Dictionary of Iranian Plant Names. Farhang Moaser, Tehran, Iran Pp 542.

Murrray MT. 1995. The healing power of herbs: the enlightened persons guide to the wonders of medicinal plants. Rocklin, CA: Prima Pub., xiv 410.

Nostro N, Germano M, Angelo VD, Cannatelli M. 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters of Applied Microbiology 30, 379-384.

Ormancey X, Sisalli S, Coutiere P. 2001. Formulation of essential oils in functional perfumery. Parfums, Cosmetiques, Actualites 157, 30–40.

Seun-Ah Y, Sang-Kyung J, Eun-Jung L, Chang-Hyun S, In-Seon L. 2010. Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Natural Products Research 24, 140 – 151.

Sokovic MD, Vukojevic J, Marin PD, Brkik DD, Van Griensven LJ. 2009. Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities. Molecules 14, 238-249.

Soltani F, Sharifi M, Khajeh K, Yousefzadi M. 2009. Study of essential oil composition, menthone reductase activity and antimicrobial activity of Mentha piperita in two stages of growth. Iranian Journal of Medicinal and Aromatic Plants 21, 62-70.

Tucker AO. 1992. The truth about mints. The Herb Companion 4, 51–52.

Yadegarinia D, Gachkar L, Bagher Rezaei M, Taghizadeh  M,  Alipoor  S,  Rasooli  I.  2006. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry 67, 1249–1255.

Yazdani D, Jamshidi AH, Mojab F. 2003. Comparative essential oil and menthol of Mentha piperita L. different origin cultivated in iran. Iranian Journal of Medicinal and Aromatic Plants 3, 73-78.