Int. J. Biosci.2(11), 74-80, November 2012
This study describes the chemical investigations of Aegle marmelos (A.marmelos), a plant belonging to the family rutaceae. Although some investigations mainly on leaves and fruits have previously been reported with this plant, but very little investigations on bark of A.marmelos have been reported to date. The stem bark of A. marmelos was extracted with organic solvent and the extracts were fractionated by using standard chromatographic techniques. A total of three compounds were isolated of which two (AM-1 & AM-5) have been isolated from different plant, but this is the first report of their occurrences in the bark of A. marmelos. The isolated pure compounds were identified by extensive analyses of their high resolution Nuclear Magnetic Resonance (1H-NMR) spectral data. The powdered stem bark of A. marmelos was extracted with methanol. Chromatographic (vacuum liquid chromatography) fractionation and purification of the crude methanolic extract yielded compound (AM-1), one coumarin (AM-5), and one sterol (AM-2). The purified compound (AM-1) was identified as Lupeol, compound (AM-5) was identified as 5-Methoxy psoralen and compound (AM-2) was identified as stigmasterol or 24-ethyl-cholesta-5- 22-dien-3beta-ol.
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