Chemical investigations of stem bark’s extracts of Aegle mermelos: (An initiative exploration pro medicinally important plant)

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Research Paper 01/11/2012
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Chemical investigations of stem bark’s extracts of Aegle mermelos: (An initiative exploration pro medicinally important plant)

Mohammad Mamunur Rahman, Md. Raihan Chowdhury, Partha Saha, Mehedy Hasan Chowdhury, S. M. Estiar Haque, Md. Saddam Nawaz
Int. J. Biosci.2( 11), 74-80, November 2012.
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Abstract

This study describes the chemical investigations of Aegle marmelos (A.marmelos), a plant belonging to the family rutaceae. Although some investigations mainly on leaves and fruits have previously been reported with this plant, but very little investigations on bark of A.marmelos have been reported to date. The stem bark of A. marmelos was extracted with organic solvent and the extracts were fractionated by using standard chromatographic techniques. A total of three compounds were isolated of which two (AM-1 & AM-5) have been isolated from different plant, but this is the first report of their occurrences in the bark of A. marmelos. The isolated pure compounds were identified by extensive analyses of their high resolution Nuclear Magnetic Resonance (1H-NMR) spectral data. The powdered stem bark of A. marmelos was extracted with methanol. Chromatographic (vacuum liquid chromatography) fractionation and purification of the crude methanolic extract yielded compound (AM-1), one coumarin (AM-5), and one sterol (AM-2). The purified compound (AM-1) was identified as Lupeol, compound (AM-5) was identified as 5-Methoxy psoralen and compound (AM-2) was identified as stigmasterol or 24-ethyl-cholesta-5- 22-dien-3beta-ol.

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Aratanchemuge, Hiroshige H, Koh S, H, Kunio I, Takashi K. 2004. Induction of apoptosis by Lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells. Journal of Oncology reports. 11,289-292.

Arul V, Kumaraguru S, Dhananjayan R.1999. Effects of aegeline and lupeol-the two cardioactive principles isolated from the leaves of Aegle marmelos Corr. J Pharm Pharmacol. 51, S252.

Basu D, Sen R. 1974. Alkaloids and coumarins from root-bark of Aegle marmelos, Phytochemistry. 13, 2329-2330.

Badam L, Bedekar SS, Sonawane KB, Joshi SP.2002. In vitro antiviral activity of bael (Aegle marmelos Corr) upon human coxsackieviruses B1-B6. J Commun Dis. 34, 88.

Chiang PK, Cantoni GL.1979. Biochem., Pharmacol. 28, 1897.

Dr. Philomena G, Nimmi OS. 2011. Cent Percent Safe Centum Plants for Antiobesity,International. Journal of Innovative Technology & Creative Eng.3, 2045-8711.

David K, Henry R, John E.1984. Hearst, 8-Methoxypsoralen-Nucleic Acid Photoreaction. Effect of Furan Photoaddition. J. Med. Chem. 27, 531-534.

Geetha T, Varalakshmi P.2001. Anti-inflammatory activity of lupeol and lupeol linoleate in rats. J Ethnopharmacol. 76, 77.

Goel RK, Maiti RN, Manickam M, Ray AB. 1997. Antiulcer activity of naturally occurring pyrano-coumarin and isocoumarins and their effect on prostanoid synthesis using human colonic mucosa. Indian J Exp Biol. 35, 1080.

Guranowski A, Montgonery JA, Cantoni GL, Chiang PK. 1981. Biochemistry 20, 110.

Jagetia GC, Venkatesh P, Baliga MS. 2005. Aegle marmelos (L.) Correa inhibits the proliferation of transplanted Ehrlich ascites carcinoma in mice. Biol Pharm Bull. 28, 58.

Jagetia GC, Venkatesh P, Baliga MS. 2003. Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of gamma-radiation: a micronucleus study, Mutagenesis. 18, 387.

Katayama T, Nagai I. 1960.Chemical significance of the volatile components of spices from the food preservative view point, IV-structure and antibacterial activity of some terpenes. Nippon Suisan Gakkaishi. 26, 29.

Khan RI. 1991. Natural Product: A Laboratory Guide, 2nd Ed. Academic Press, N.Y., USA.

Kumar DS, Prabhakar YS. 1987. On the ethnomedical significance of the arjun tree, Terminalia arjuna (Roxb.), Wight & Arnot. Journal of Ethnopharmacology. 20(2),173-90.

Mishra SB, Rao CV, Ojha SK, Vijayakumar M, Verma A. 2010. An Analytical Review of Plants for Anti Diabetic Activity with their Phytoconstituent & Mechanism of Action. Internatinal Journal of Pharmaceutical Sciences & Research. 1(1).

Misra P, Pal NL, Guru PY, Katiyar JC, Tandon JS.1991. Antimalarial activity of traditional plants against erythrocytic stages of Plasmodium berghei. Int J Pharmacog, 29, 19.

Parasakthy K, Deepalakshmi PD, Shanthy S, Niranjali SD.1993. The hepatoprotective action of eugenol. Med Sci Res. 21, 611.

Rastogi RP, Mehrotra BN.1993. In Compendium of Indian medicinal plants, Volume 3, edited by Rastogi RP (C.D.R.I. Lucknow & Pubications & Information Directorate, New Delhi).

Rastogi RP, Mehrotra BN. 1995. In Compendium of Indian medicinal plants, Volume 4, edited by Rastogi R P (C.D.R.I., Lucknow & Pubications & Information Directorate, New Delhi).

Suleyman A, Sema A. 2009. Investigation of In vitro Antimicrobial Activity of Aloe vera Juice. Journal of Animal and Veterinary Advances. 8, 99-102.

Takase H, Yamamoto K, Hirano H, Saito Y ,Yamashita A. 1994. Pharmacological profile of gastric mucosal protection by marmin and nobiletin from a traditional herbal medicine. J Pharmacol. 66, 139.

Vimal V, Devaki T. 2004. Linear furano coumarin protects rat myocardium against lipidperoxidation and membrane damage during experimental myocardial injury. Biomed Pharmacother. 58, 393.