Chitin extraction from sardine fish scales

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Research Paper 15/11/2022
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Chitin extraction from sardine fish scales

Sheeva Yahcob-Saddalani, Janus Pansacala, Joel Fernando, Reynan Toledo
Int. J. Biosci.21( 5), 216-222, November 2022.
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Fish scale is a waste from the canning industry that is abundant in Zamboanga City, Philippine. In this study, the fish scale is converted into a high-value product of chitin through deproteination and demineralization. For deproteination, the concentration of sodium hydroxide and reaction time were varied at constant temperature of 80°C and sample-to-reagent ratio of 1:10. The deproteinized sample undergone demineralization setting concentration of hydrochloric acid and temperature constant while varying the sample-to-reagent ratio and reaction time. Results revealed a deproteination parameters of 4N NaOH, 1:10 sample-to-reagent ratio, 2 hrs reaction time at 80°C and demineralization parameters of 0.75 N HCl, 1:6 sample-to-reagent ratio, 72 hrs reaction time at room temperature produced 63.66(±0.12)% chitin. It is concluded that chitin was successfully extracted from sardine fish scales which is of high-value product in different industries.


Aguilor UP, Raju R, Raju J, Capili JT. 2022. Characterization of Chitosan Extracted from Exoskeletons of Pomacea spp. International Journal of Biosciences 21(1), 184-191.

Aranaz I, Mengíbar M, Harris R, Paños I, Miralles B, Acosta N, Heras Á. 2009. Functional Characterization of Chitin and Chitosan. Current Chemical Biology 3(2), 203-230.

Arvanitoyannis IS, Kassaveti A. 2008. Fish industry waste: Treatments, environmental impacts, current and potential uses. International Journal of Food Science and Technology 43(4), 726-745.

Awarenet. 2004. Handbook for the prevention and minimization of waste and valorizationof by-products in European agro-food industries. Grow Programme, European Commission.

Barikani M, Oliaei E, Seddiqi H, Honarkar H. 2014. Preparation and application of chitin and its derivatives: A review. Iranian Polymer Journal (English Edition).

Boarin Alcalde L, Graciano Fonseca G. 2016. Alkali process for chitin extraction and chitosan production from Nile tilapia (Oreochromis niloticus) scales. Latin American Journal of Aquatic Research 44(4), 683-688.

Chang KTB, Tsai G. 1997. Response Surface Optimization and Kinetics of Isolating Chitin from Pink Shrimp Shell Waste. Journal of Agricultural and Food Chemistry 45, 1900-1904.

Chang WT, Chen YC, Jao CL. 2007. Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes. Bioresource Technology 98(6), 1224–1230. /10.1016 /j.biortech.2006.05.005 .

Fernandez Kim S. 2004. Physicochemical and Functional Properties of Crawfish Chitosan as Affected by Different Processing Protocols, Thesis, Lousiana State University.

Ferraro V, Cruz IB, Jorge RF, Malcata FX, Pintado ME, Castro PML. 2010. Valorisation of natural extracts from marine source focused on marine by-products: A review. Food Research International. Elsevier Ltd. 10.1016 /j.foodres.2010.07.034

Hardy RW, Tacon AGJ. 2002. Fish meal: Historical uses, production trends and future outlook for sustainable supplies. In R. R. Stickney & J. P. McVey (Eds.), Responsible Marine Aquaculture pp. 311-326. USA.

Hayes M, Carney B, Slater J, Brück W. 2008. Mining marine shellfish wastes for bioactive molecules: Chitin and chitosan; Part A: extraction methods. Biotechnology Journal 3(7), 871-877.

Iqbal J, Wattoo FH, Wattoo MHS, Malik R, Tirmizi SA, Imran M, Ghangro AB. 2011. Adsorption of acid yellow dye on flakes of chitosan prepared from fishery wastes. Arabian Journal of Chemistry 4(4), 389-395. /j.arabjc. 2010.07.007.

Kumari S, Rath P, Sri Hari Kumar A. 2016. Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeonohita): Extractiona and Characterization. African Journal of Biotechnology 15(24), 1258-1268. /AJB 2015. 15138.

Meyers MA, Chen PY, Lin AYM, Seki Y. 2008. Biological materials: Structure and mechanical properties. Progress in Materials Science 53(1), 1-206.

Muslim T, Rahman MH, Begum HA, Rahman MA. 2013. Chitosan and Carboxymethyl Chitosan from Fish Scales of Labeo rohita. J. Sci 61(1), 145-148.

Narvaez T, Gangan A. 2014. Supply chain improvement of sardines in selected region in the Philippines (Zamboanga peninsula). Philippines: Philippine Center for Agriculture, Aquatics, and Natural Resources Research and Development. DOST.

Poeloengasihhernawan C, Suharto S, Kismurtono M. 2009. Proceedings of National Seminar on Applied Technology, Science, and Arts (1st APTECS), Surabaya, 22 Dec. 2009, ISSN 2086-1931 861, Optimization of Chitin Production from Penaeus monodon Shells at Ambient Temperature

Rinaudo M. 2006. Chitin and chitosan: Properties and applications. Progress in Polymer Science (Oxford). 2006. 06.001.

Rustad T. 2003. Utilization of marine by-product. Electronic Journal of Enviromental, Agricltural and Food Chemistry 2(4), 458-463.

Siddique F, Arshad M, Hassan W, Shah ZH, Akhtar U. A review on Chitosan: As a potent fish preservative. International Journal of Biosciences 16(4), 90-101. /ijb/16.4.

Stepnowski P, Ólafsson G, Helgason H, Jastorff B. 2004. Recovery of astaxanthin from seafood wastewater utilizing fish scales waste. Chemosphere 54(3), 413-417. 10.1016/ S0045- 6535(03)00718-5.

Suneeta K, Rath P, Sri HKA. 2016. Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeorohita): Extraction and characterization. African Journal of Biotechnology 15(24), 1258-1268.

Tang WJ, Fernandez JG, Sohn JJ, Amemiya CT. 2015. Chitin is endogenously produced in vertebrates. Current Biology 25(7), 897-900.

Toan NV, Ng CH, Aye KN, Trang TS, Stevens WF. 2006. Production of high-quality chitin and chitosan from preconditioned shrimp shells. J. Chem. Technol. Biotechnol 81, 1113-1118. /10.1002 /jctb.1437.

Tokatl K, Demirdöven A. 2018. Optimization of chitin and chitosan production from shrimp wastes and characterization. J Food Process Preserv 42, e13494.

Whitehead PJ. 1985. Clupeoid Fishes of the World (Suborder Clupeoidei): An Annotated and illustrated catalogue of the herrings, sardines, pilchards, sprats, shads, anchovies and wolf-herrings. FAO Species Catalogue 7, 90-114.

Willette D, Bognot E, Mutia M, Santos M. 2011. Biology and ecology of sardines in the Philippines: a review. BFAR Technical Paper Series 13, 1-20.

Younes I, Rinaudo M. 2015. Chitin and Chitosan Preparation from Marine Sources. Structure, Properties and Applications. Marine Drugs 13(3), 1133-1174.

Zaku SG, Aguzue SAEOC, Thomas SA. 2011. Extraction and characterization of chitin ; a functional biopolymer obtained from scales of common carp fish (Cyprinus carpio L.): A lesser known source. African Journal of Food Science 5(8), 478-483.