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Citrus budwood treatment with antibiotic and temperature to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

Research Paper | August 1, 2019

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Muhammad Sarwar Yaqub, Iqrar Ahmad Khan, Rozina Aslam

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Int. J. Biosci.15( 2), 150-159, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.150-159


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Citrus trees infected by the Candidatus liberibacter asiaticus the causal organism of huanglongbing (HLB) either by its natural host Diaphorina citri kuwayama (Hemiptera: Psyllidae) or during budding/grafting process are difficult to treat in the field and green house and nurseries by cultural practices. In the controlled conditions of growth room, sweet orange budwood infected with Candidatus Liberibacter asiaticus were treated with tetracycline and temperature simultaneously in this study to eliminate the bacterium with the objective that the pathogen elimination from the budwood could be comparatively more effective. Tetracycline doses used were 250 ppm, 500 ppm, 750 ppm, 1000 ppm and 1250 ppm while a temperature was kept constant at 50 °C for all doses of tetracycline.  Budwood source trees were confirmed for HLB bacterium existence by conventional PCR using 16S rDNA primers OI1/OI2C and rplKAJL-rpoBC operon primers A2/J5. Budsticks treated with the above mentioned doses of antibiotics were grafted on rough lemon plants. Real time PCR analysis results of plants grafted with treated budwood revealed no amplification in healthy control as well as 1000ppm tetracycline + 50°C treatment indicating no detectable titer in the said treatment. These results provide a hope in the darkness to save the citrus industry of the world.


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Citrus budwood treatment with antibiotic and temperature to control Candidatus Liberibacter asiaticus transmission for huanglongbing management

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