Classification of local banana Mulu Bebe North maluku based on rpoC genes and trnF–trnH intron

Paper Details

Research Paper 01/05/2014
Views (748)
current_issue_feature_image
publication_file

Classification of local banana Mulu Bebe North maluku based on rpoC genes and trnF–trnH intron

Adriana Hiariej, Estri Laras Arumingtyas , Arik Arubil Fatina
J. Biodiv. & Environ. Sci. 4(5), 266-272, May 2014.
Copyright Statement: Copyright 2014; The Author(s).
License: CC BY-NC 4.0

Abstract

North Maluku has a distinctive banana plant, the name of the plant is determined by the local community. The local name is Mulu Bebe due to the shape of the fruit’s edges resembles a duck’s mouth. Previously, there are no data on the existing Mulu Bebe bananas in other parts of Indonesia, which means Mulu Bebe banana fathomed as typical species of North Maluku. Information on Mulu Bebe banana are limited and the newest report was only on physical and morphological variability. Morphological features observed on previous study in 2012, determined Mulu Bebe as Musa AA group banana. This study aimed to classify Mulu Bebe banana based on its molecular characters. This study was conducted from October to December 2013 in the Laboratory of Molecular Biology, University of Brawijaya. Molecular markers used in this study is the chloroplast DNA (cpDNA), especially the sequence coding region (rpoC1) and non-coding regions (trnF-trnH introns). The results of molecular analysis using gene rpoC1 and trnF – trnH Intron indicate local banana Mulu Bebe in North Maluku include in Musa acuminata group with a similarity value of 100%.

Baumgarthner BJ, Rapp JC, Mullet JE. 1993. Plastid genes encoding the transcription/translation apparatus are differentially transcribes early in barley (Hordeum vulgare) chloroplast development. Plant Physiology 101, 781-791.

CBOL. 2009. CBOL approves matK and rbcL as the DNA barcode regions for land plants. Executive Committee, CBOL Proceedings of the National Academy of Science.

Chakravarthi BK, Nuravaneni R. 2006. SSR marker based DNA fingerprinting and diversity study ini rice (Oryza sativa L.). African Journal of Biotechnology 5 (9), 684-688.

Clegg MT, Gautt BS, Learn GHJr, Morton BR. 1994. Rates and pattern of chloroplast DNA evolution. Proceeding of National Academy of Science USA 91, 6795-6801.

Clegg MT, Zurawski G. 1991. Chloroplast DNA and study of plant phylogeny. In: Soltis PS, Soltis DE, Doyle JJ, Eds. Molecular systematics of plants. New York: Champman and Hall.

De Jesus ON, de O. Silva S, Amorin EP, Ferreira CF, de Campos JMS, de G. Silva G, Figueira A. 2013. Genetic diversity and population structure of Musa accessions in ex situ conservation. BMC Plant Biology 13, 41.

De Las Rivas J, Lozano JJ, Ortiz AR. 2002. Comparative analysis of chloroplast genomes: functional annotation, genome based phylogeny, and deduced evolutionary patterns. Genome Research 2 (30), 567-583.

Ferdous J, Hanafi MM, Rafi MY, Muhammad K. 2012. A quick DNA extraction protocol without liquid nitrogen in ambient temperature. African Journal of Biotechnology 11 (27), 6956 – 6964

Hidayat Y, Indra HH, Miskat R, Syahbuddin H. 2012. Investigation on physical and morphology performance of Mulu Bebe Banana. North Maluku: BPTP.

Jonah PM, Bello LL, Lucky O, Midau A, Moruppa SM. 2011. Review: The importance of molecular markers in plant breeding programmes. Global Journal of Science Frontier Research 11 (5), 1-9.

Judd WS, Campbell CS, Kellog EA, Stevens PF, Donoghue MJ. 2002. Plant Systematics: A Phylogenetic approach, 2nd Edition. Sunderland: Sinauer Associates.

Megia R. 2005. Musa as a genome model. Jurnal Hayati 12 (4), 167-170.

Purseglove JW. 1985. Tropical crops Monocotyledons. London: Logman.

Roy SW, Penny D. 2007. Patterns of intron loss and gain in plants: intron loss-dominated evolution and genome-wide comparison of O. sativa and A. thaliana. Molecular Biology evolution 24 (1), 171-181.

Saski C, Seung-Bum L, Dniell H, Wood TC, Tomkins J, Hyi-Gyung K, Jansen RK. 2005. Complete chloroplast genome sequence of Glycine max and comparative analyses with other legume genome. Plant Molecular Biology 59, 309–322.

Simpson MG. 2006. Plant  systematics.  Canada: Elsevier Academic Press.

Stover RH, Simmonds NW. 1987. Banana. 3rd Ed. London: Logman.

Sunyoto A. 2011. Cultivation of Cavendish Banana. Jogjakarta: Berlian Media.

Susan  H.  2003.  Bootstrapping  Phylogenetic  trees: theory and method. Statistical Science 18 (2), 241–255

Ubaidillah R, Sutrisno H. 2009. Introduction to Biosystematics theory and practices. Biology Research Center. Bogor: Indonesian Institute of Science.

Wahyuningtyas W, Retno A, Rahayu SE. 2009. Genetics diversity of B genome banana based on Microsatellite Markers. Biosaintifika 1 (1), 1-10.

Wolfe KH, Li WH, Sharp PM. 1987. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proceeding of National Academy of Science USA 84, 9054-9058.

Related Articles

Value chain analysis and business model development of the Rizal mango growers agriculture cooperative

Macluven T. Gonzales, Diana O. Lim*, Karen Joy A. Abalos, J. Biodiv. & Environ. Sci. 29(1), 186-198, July 2026.

Susceptibility of Culex quinquefasciatus to insecticides in the industrial areas of Koumassi, Vridi, and Yopougon in Abidjan, Ivory Coast, during the rainy season

Gahapie Urbain Silue*, Koffi Ladji Yao, N’tamon Romeo N’tamon, Edmond Charles Basseli, Genevieve Lydie Acapovi-Yao, Yao Lucien Konan, J. Biodiv. & Environ. Sci. 29(1), 175-185, July 2026.

Optimization of maltodextrin as a bulking agent in mango (Mangifera indica) fruit bar: Physical, sensory, and nutritional evaluation

Macluven T. Gonzales*, Diana O. Lim, Jocelyn D. Tuliao, Hitler C. Dangatan, J. Biodiv. & Environ. Sci. 29(1), 150-163, July 2026.

An assessment of the effect of wind flow on the indoor thermal condition of some buildings in Benin City, Nigeria

Tashok, Yusuf Haruna*, J. Biodiv. & Environ. Sci. 29(1), 136-149, July 2026.

Analyzing roadside tree diversity reveals dominance, shadow diversity, and management archetypes: The case of Tagbilaran City, Bohol, Philippines

Johnuel Tac-on, Jesie Diola, Noel T. Lomosbog, Jairyl B. Oclarit, MA. Aneli A. Lanzaderas*, J. Biodiv. & Environ. Sci. 29(1), 122-135, July 2026.

Floristic diversity of major river systems in Zamboanga del Norte

Ma. Dulce C. Guillena*, Maria Rio A. Naguit, Ethel T. Cata-al, Tessie G. Pulido, J. Biodiv. & Environ. Sci. 29(1), 101-111, July 2026.