Classification of local banana Mulu Bebe North maluku based on rpoC genes and trnF–trnH intron

Paper Details

Research Paper 01/05/2014
Views (702)
current_issue_feature_image
publication_file

Classification of local banana Mulu Bebe North maluku based on rpoC genes and trnF–trnH intron

Adriana Hiariej, Estri Laras Arumingtyas , Arik Arubil Fatina
J. Biodiv. & Environ. Sci. 4(5), 266-272, May 2014.
Copyright Statement: Copyright 2014; The Author(s).
License: CC BY-NC 4.0

Abstract

North Maluku has a distinctive banana plant, the name of the plant is determined by the local community. The local name is Mulu Bebe due to the shape of the fruit’s edges resembles a duck’s mouth. Previously, there are no data on the existing Mulu Bebe bananas in other parts of Indonesia, which means Mulu Bebe banana fathomed as typical species of North Maluku. Information on Mulu Bebe banana are limited and the newest report was only on physical and morphological variability. Morphological features observed on previous study in 2012, determined Mulu Bebe as Musa AA group banana. This study aimed to classify Mulu Bebe banana based on its molecular characters. This study was conducted from October to December 2013 in the Laboratory of Molecular Biology, University of Brawijaya. Molecular markers used in this study is the chloroplast DNA (cpDNA), especially the sequence coding region (rpoC1) and non-coding regions (trnF-trnH introns). The results of molecular analysis using gene rpoC1 and trnF – trnH Intron indicate local banana Mulu Bebe in North Maluku include in Musa acuminata group with a similarity value of 100%.

Baumgarthner BJ, Rapp JC, Mullet JE. 1993. Plastid genes encoding the transcription/translation apparatus are differentially transcribes early in barley (Hordeum vulgare) chloroplast development. Plant Physiology 101, 781-791.

CBOL. 2009. CBOL approves matK and rbcL as the DNA barcode regions for land plants. Executive Committee, CBOL Proceedings of the National Academy of Science.

Chakravarthi BK, Nuravaneni R. 2006. SSR marker based DNA fingerprinting and diversity study ini rice (Oryza sativa L.). African Journal of Biotechnology 5 (9), 684-688.

Clegg MT, Gautt BS, Learn GHJr, Morton BR. 1994. Rates and pattern of chloroplast DNA evolution. Proceeding of National Academy of Science USA 91, 6795-6801.

Clegg MT, Zurawski G. 1991. Chloroplast DNA and study of plant phylogeny. In: Soltis PS, Soltis DE, Doyle JJ, Eds. Molecular systematics of plants. New York: Champman and Hall.

De Jesus ON, de O. Silva S, Amorin EP, Ferreira CF, de Campos JMS, de G. Silva G, Figueira A. 2013. Genetic diversity and population structure of Musa accessions in ex situ conservation. BMC Plant Biology 13, 41.

De Las Rivas J, Lozano JJ, Ortiz AR. 2002. Comparative analysis of chloroplast genomes: functional annotation, genome based phylogeny, and deduced evolutionary patterns. Genome Research 2 (30), 567-583.

Ferdous J, Hanafi MM, Rafi MY, Muhammad K. 2012. A quick DNA extraction protocol without liquid nitrogen in ambient temperature. African Journal of Biotechnology 11 (27), 6956 – 6964

Hidayat Y, Indra HH, Miskat R, Syahbuddin H. 2012. Investigation on physical and morphology performance of Mulu Bebe Banana. North Maluku: BPTP.

Jonah PM, Bello LL, Lucky O, Midau A, Moruppa SM. 2011. Review: The importance of molecular markers in plant breeding programmes. Global Journal of Science Frontier Research 11 (5), 1-9.

Judd WS, Campbell CS, Kellog EA, Stevens PF, Donoghue MJ. 2002. Plant Systematics: A Phylogenetic approach, 2nd Edition. Sunderland: Sinauer Associates.

Megia R. 2005. Musa as a genome model. Jurnal Hayati 12 (4), 167-170.

Purseglove JW. 1985. Tropical crops Monocotyledons. London: Logman.

Roy SW, Penny D. 2007. Patterns of intron loss and gain in plants: intron loss-dominated evolution and genome-wide comparison of O. sativa and A. thaliana. Molecular Biology evolution 24 (1), 171-181.

Saski C, Seung-Bum L, Dniell H, Wood TC, Tomkins J, Hyi-Gyung K, Jansen RK. 2005. Complete chloroplast genome sequence of Glycine max and comparative analyses with other legume genome. Plant Molecular Biology 59, 309–322.

Simpson MG. 2006. Plant  systematics.  Canada: Elsevier Academic Press.

Stover RH, Simmonds NW. 1987. Banana. 3rd Ed. London: Logman.

Sunyoto A. 2011. Cultivation of Cavendish Banana. Jogjakarta: Berlian Media.

Susan  H.  2003.  Bootstrapping  Phylogenetic  trees: theory and method. Statistical Science 18 (2), 241–255

Ubaidillah R, Sutrisno H. 2009. Introduction to Biosystematics theory and practices. Biology Research Center. Bogor: Indonesian Institute of Science.

Wahyuningtyas W, Retno A, Rahayu SE. 2009. Genetics diversity of B genome banana based on Microsatellite Markers. Biosaintifika 1 (1), 1-10.

Wolfe KH, Li WH, Sharp PM. 1987. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proceeding of National Academy of Science USA 84, 9054-9058.

Related Articles

Floristic composition and woody species diversity in Campo-Ma’an National Park, South Cameroon

Achey Nkenfack Djike Baudelair*, Temgoua Lucie Félicité, Kuete Fogang Marcien, Nfondem Poumie Mohamed Mounir, Atoupka Abdel Malik, Djeuni Duplex Romuald, Kontchiachou Nkana Didier, J. Biodiv. & Environ. Sci. 28(6), 103-119, June 2026.

Comparative effects of bio-inoculant on nutrient dynamics of biodegradable waste

Anjelle-J G. Debosura*, Carlo Stephen O. Moneva, Corazon V. Ligaray, Elizabeth Edan M. Albiento, MA. Cecilia V. Almeda, Melgie A. Alas, Frandel Louis S. Dagoc, Peter D. Suson, J. Biodiv. & Environ. Sci. 28(6), 97-102, June 2026.

Impact of deforestation on the aquatic macroinvertebrate community and the ecological quality of Mé River (South-East, Côte d’Ivoire)

Gnago Dohou Affri*, Tapé Logboh David, Edia Oi Edia, J. Biodiv. & Environ. Sci. 28(6), 80-96, June 2026.

Vulnerability and regeneration potential of Bambusa vulgaris in Ebolowa, South Cameroon

Rodine Tchiofo Lontsi*, Duchesse Elvira Kepmou, Emilienne Laure Ngahane, Jacques Christophe Awoa Essam, Isaac Blaise Djoko, J. Biodiv. & Environ. Sci. 28(6), 68-79, June 2026.

Temporal availability of floral resources for the honey bee (Apis mellifera) in a forest ecosystem in the sudanian zone of Côte d’Ivoire: The case of Badenou classified forest

Dofoungo Koné*, Comlan Mawussi Koudegnan, Siendou Coulibaly, Fofana Séguéna, Bruno Marcel Iritié, Wandan Eboua Narcisse, J. Biodiv. & Environ. Sci. 28(6), 56-67, June 2026.

Carbon sequestration potential of napier (Pennisetum purpureum) grass applied with varying classifications of livestock excrement

Alliah B. Balaba*, Niña Mae R. Villar, Ana Celina T. Soriano, Myrna G. Pabiona, J. Biodiv. & Environ. Sci. 28(6), 50-55, June 2026.

Effects of environmental stressors on morphological traits of Glycine max (L.) Merr. and microbial diversity of soil treated with cadmium and lead

Fortune Onyeuka Otumunye*, Tobore Roseline Agbosa, Boniface Edegbai, J. Biodiv. & Environ. Sci. 28(6), 42-49, June 2026.

SWAT+-based water balance assessment of Ipil watershed in Bohol, Philippines: Spatial and temporal patterns of water availability

Anselmo M. Aurestila*, Proceso M. Castil, Manolito C. Macalolot, J. Biodiv. & Environ. Sci. 28(6), 30-41, June 2026.