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Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch

Qiling Song, Feng Xu, Tingting Tao, Xiaomeng Liu, Weiwei Zhang, Li Zhu, Guiyuan Wang

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Int. J. Biosci.7(5), 136-145, November 2015

DOI: http://dx.doi.org/10.12692/ijb/7.5.136-145

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Abstract

Anthocyanins as a type of plant flavonoids, is a kind of important plant secondary metabolites, which is catalyzed by a series of enzymes such as flavanone 3-hydroxylase (F3H). Here a F3H gene, designated as PpF3H, was cloned from Prunus persica (L.) Batsch. The full-length cDNA of PpF3H gene was 1453 bp in length, containing a 1086 bp open reading frame (ORF) which encodes 361-amino-acid proteins with a calculated molecular weight of 40.50 kDa and isoelectric point of 5.45. Protein analysis and phylogenetic tree analysis indicated that PpF3H shared the same ancestor in evolution with other F3Hs and had a further relationship with other angiosperms species. Use SWISS-MODEL to perform three-dimensional structure, the results showed that PpF3H had a jerry roll in the enzyme core consisted of β-fold, and all of the 2-oxoglutarate-dependent dioxygenase share a common structure. The model analysis showed that the PpF3H protein contains 17 α-helixes and 17 β-sheets. Molecular cloning and sequence analysis of PpF3H were carried out to further study the molecular mechanisms of biosynthese of anthocyanins.

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Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch

Boss PK, Davies C, Robinson SP. 1996. Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L. cv Shiraz grape berries and the implications for pathway regulation. Plant Physiology 111, 1059-1066. http://dx.doi.org/10.1104/pp.111.4.1059

Chai CY, Hu DX. 2003. Yixing lily characteristics and high yield cultivation technique. Journal of Guangxi Gardening, 3, 24-25. http://dx.doi.org/10.3969/j.issn.16745868.2003.03.014

Charrier B, Coronado C, Kondorosi A, Ratet P. 1995. Molecular characterization and expression of alfalfa (Medicago sativa L.) flavanone-3-hydroxylase and dihydroflavonol-4-reductase encoding genes. Plant Molecular Biology 29, 773-786. http://dx.doi.org/10.1007/BF00041167

Cultrone A, Cotroneo PS, Recupero GR. 2010. Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis. Tree Genetics & Genomes 6, 101-112. http://dx.doi.org/10.1007/s11295-009-0232-y

Debeaujon I, Léon-Kloosterziel KM, Koornneef M. 2000. Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. Plant Physiology 122, 403-414. http://dx.doi.org/10.1104/pp.122.2.403

Deboo GB, Albertsen MC, Taylor LP. 1995. Flavanone 3-hydroxylase transcripts and flavonol accumulation are temporally coordinate in maize anthers. The Plant Journal 7, 703-713. http://dx.doi.org/10.1046/j.1365-313X.1995.07050703.x

Dooner HK, Robbins TP, Jorgensen RA. 1991. Genetic and developmental control of anthocyanin biosynthesis. Annual Review of Genetics 25, 173-199. http://dx.doi.org/10.1146/annurev.ge.25.120191.0011 33

El Kereamy A, Chervin C, Souquet JM. 2002. Ethanol triggers grape gene expression leading to anthocyanin accumulation during berry ripening. Plant Science 163, 449-454. http://dx.doi.org/10.1016/S0168-9452(02)00142-5

Elomaa P, Honkanen J, Puska R, Seppänen P, Helariutta Y, Mehto M, Kotilainen M, Nevalainen L, Teeri TH. 1993. Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Nature Biotechnology 11, 508–511. http://dx.doi.org/10.1038/nbt0493-508

Flügge UI, Gao W. 2005. Transport of isoprenoid intermediates across chloroplast envelope membranes. Plant Biology 7, 91-97. http://dx.doi.org/10.1055/s-2004-830446

Grotewold E, Jin Z, Qu W. 2005. Cloning and characterization of a flavanone 3-hydroxy lase gene from Saussurea medusa. Mitochondrial DNA 16, 121-129. http://dx.doi.org/10.1080/10425170500050742

Hahlbrock K, Scheel D. 1989. Physiology and molecular biology of phenylpropanoid metabolism. Annual Review of Plant Biology 40, 347-369. http://dx.doi.org/10.1146/annurev.pp.40.060189.00 2023

Holton TA, Cornish EC. 1995. Genetics and biochemistry of anthocyanin biosynthesis. The Plant Cell 7, 1071. http://dx.doi.org/10.1105/tpc.7.7.1071

Honda C, Kotoda N, Wada M. 2002. Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin. Plant Physiology and Biochemistry 40, 955-962. http://dx.doi.org/10.1016/S0981-9428(02)01454-7

Hu XJ, Xu YJ, Gao LP. 2014. Tea flavanone 3- hydroxylase gene (F3H) cloning and functional analysis. Journal of Agricultural Biotechnology 22, 309-316. http://dx.doi.org/10.3969/j.issn.16747968.2014.03.006

Jeong ST, Goto-Yamamoto N, Hashizume K. 2006. Expression of the flavonoid 3′-hydroxylase and flavonoid 3′,5′-hydroxylase genes and flavonoid composition in grape (Vitis vinifera). Plant Science 170, 61-69. http://dx.doi.org/10.1016/j.plantsci.2005.07.025

Kim  SH,  Lee  JR,  Hong  ST.  2003.  Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin. Plant Science 165, 403-413. http://dx.doi.org/10.1016/S0168-9452(03)00201-2

Koes R, Verweij W, Quattrocchio F. 2005. Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends in Plant Science 10, 236-242. http://dx.doi.org/ 10.1016/j.tplants.2005.03.002

Koes RE, Quattrocchio F, Mol JNM. 1994. The flavonoid biosynthetic pathway in plants: function and evolution. BioEssays 16, 123-132. http://dx.doi.org/10.1002/bies.950160209

Kumar S, Tamura K, Nei M. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163. http://dx.doi.org/10.1093/bib/5.2.150

Li J, Ou-Lee T M, Raba R. 1993. Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. The Plant Cell 5, 171-179. http://dx.doi.org/10.1105/tpc.5.2.171

Nakatsuka T, Nishihara M, Mishiba K. 2006. Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants. Molecular Breeding 17, 91-99. http://dx.doi.org/10.1007/s11032-005-2520-z

Peer  WA,  Bandyopadhyay  A,  Blakeslee  JJ. 2004. Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana. The Plant Cell 16, 1898-1911. http://dx.doi.org/10.1105/tpc.021501

Pelletier MK, Shirley BW. 1996. Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings (Coordinate regulation with chalcone synthase and chalcone isomerase). Plant Physiology 111, 339-345. http://dx.doi.org/10.1104/pp.111.1.339

Pelt JL, Downes WA, Schoborg RV. 2003. Flavanone 3-hydroxylase expression in Citrus paradisi and Petunia hybrida seedlings. Phytochemistry 64, 435-444. http://dx.doi.org/10.1016/S0031-9422(03)00341-8

Shen G, Pang Y, Wu W. 2006. Cloning and characterization of a flavanone 3-hydroxylase gene from Ginkgo biloba. Bioscience Reports 26, 19-29. http://dx.doi.org/10.1007/s10540-006-9007-y

Shirley BW. 2001. Flavonoid biosynthesis: A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology 126, 485-493. http://dx.doi.org/10.1104/pp.126.2.485

Singh K, Rani A, Kumar S. 2008. An early gene of the flavonoid pathway, flavanone 3-hydroxylase, exhibits a positive relationship with the concentration of catechins in tea (Camellia sinensis). Tree Physiology 28, 1349-1356. http://dx.doi.org/10.1093/treephys/28.9.1349

Testolin R, Marrazzo T, Cipriani G. 2000. Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. Genome 43, 512-520. http://dx.doi.org/10.1139/g00-010

Toda K, Yang D, Yamanaka N. 2002. A single-base deletion in soybean flavonoid 3′-hydroxylase gene is associated with gray pubescence color. Plant Molecular Biology 50, 187-196. http://dx.doi.org/10.1023/A:1016087221334

Van Der Meer IM, Stam ME, van Tunen AJ. 1992. Antisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterility. The Plant Cell 4, 253-262. http://dx.doi.org/10.1105/tpc.4.3.253

Wasson AP, Pellerone FI, Mathesius U. 2006. Silencing the flavonoid pathway in Medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia. The Plant Cell 18, 1617-1629. http://dx.doi.org/10.1105/tpc.105.038232

Welford RWD, Clifton IJ, Turnbull JJ. 2005. Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism. Organic & Biomolecular Chemistry 3, 3117-3126. http://dx.doi.org/10.1039/B507153D

Xu F, Li L, Chen H, Tang Y, Cheng SY, Zhang WW. 2010.Cloning and sequence analysis of a flavanone 3-hydroxylase cDNA 3′ End from Juglans regia. Journal of Northwest Agriculture and Forestry 19, 151-157 http://dx.doi.org/10.3969/j.issn.10041389.2010.01.0 34

Zhang H, Zhao L, Wang J. 2014. Cloning and functional  analysis  of  two  flavanone-3-hydroxylase genes  from  Reaumuria  trigyna.  Acta  Physiologiae Plantarum 36, 1221-1229. http://dx.doi.org/10.1007/s11738-014-1499-7

Zuker A, Tzfira T, Ben-Meir H. 2002. Modification of flower color and fragrance by antisense suppression of the flavanone 3-hydroxylase gene. Molecular Breeding 9, 33-41. http://dx.doi.org/10.1023/A:1019204531262

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