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Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch

Qiling Song, Feng Xu, Tingting Tao, Xiaomeng Liu, Weiwei Zhang, Li Zhu, Guiyuan Wang

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Int. J. Biosci.7(5), 136-145, November 2015

DOI: http://dx.doi.org/10.12692/ijb/7.5.136-145


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Anthocyanins as a type of plant flavonoids, is a kind of important plant secondary metabolites, which is catalyzed by a series of enzymes such as flavanone 3-hydroxylase (F3H). Here a F3H gene, designated as PpF3H, was cloned from Prunus persica (L.) Batsch. The full-length cDNA of PpF3H gene was 1453 bp in length, containing a 1086 bp open reading frame (ORF) which encodes 361-amino-acid proteins with a calculated molecular weight of 40.50 kDa and isoelectric point of 5.45. Protein analysis and phylogenetic tree analysis indicated that PpF3H shared the same ancestor in evolution with other F3Hs and had a further relationship with other angiosperms species. Use SWISS-MODEL to perform three-dimensional structure, the results showed that PpF3H had a jerry roll in the enzyme core consisted of β-fold, and all of the 2-oxoglutarate-dependent dioxygenase share a common structure. The model analysis showed that the PpF3H protein contains 17 α-helixes and 17 β-sheets. Molecular cloning and sequence analysis of PpF3H were carried out to further study the molecular mechanisms of biosynthese of anthocyanins.


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Cloning and sequence analysis of a flavanone 3-hydroxylase gene from Prunus persica (L.) Batsch

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