Cloning and sequence analysis of GbCHS6 gene from Ginkgo biloba
Paper Details
Cloning and sequence analysis of GbCHS6 gene from Ginkgo biloba
Abstract
Chalcone synthase (CHS) is a key enzyme in the flavonoids biosynthetic pathway. In this study, chalcone synthase gene from Ginkgo biloba (GbCHS6) was cloned. The cDNA fragment of GbCHS6 was 1191 bp, which encodes 396-amino-acid proteins with a calculated molecular weight of 43.29 kDa and isoelectric point of 6.47. Multiple alignments showed the amino acid sequence of GbCHS6 have extensive homology with those of CHS proteins from other plant. Phylogenetic tree analysis revealed that GbCHS6 had closer relationship with CHSs from Pinaceae and Cupressaceae plants than other plants. Molecular cloning and sequence analysis of GbCHS6 were conducted to further investigate the molecular mechanisms of biosynthese of flavonoids.
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