CO1 based DNA barcoding of some pentatomomorpha bugs (Hemiptera: Heteroptera) from Swat, Pakistan

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Research Paper 01/10/2018
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CO1 based DNA barcoding of some pentatomomorpha bugs (Hemiptera: Heteroptera) from Swat, Pakistan

Sana Ullah, Habib Ahmad, Muhammad Ather Rafi
Int. J. Biosci.13( 4), 139-148, October 2018.
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DNA barcodes are useful for the identification of species. This study is an expansion of the database of barcodes by adding some cytochrome oxidase I (COI) gene sequences of Pentatomomorpha species of Pakistan. The COI gene sequences obtained in this study were analyzed for nucleotide composition and divergence at intraspecific, interspecific and intergeneric level. Phylogenetic trees were constructed by Neighbor Joining, Maximum Likelihood and Minimum Evolution methods using the MEGA 7 software. COI gene sequences of thirteen species were deposited to Barcode of Life Data System (BOLD). Out of the total sequences for eight species, namely, Hotea curculionoides Herrich-Schaeffer, Adria parvula Dallas, Neohalys acuticornis Ahmad and Perveen, Scotinophara ochracea Distant, Lachnesthus singalensis Dohrn, Rhyparothesus dudgeoni Distant, Neophysopelta schlanbuschi Ahmad & Abbas, Homoeocerus sigilatus Stal this is the first molecular study which has generated distinct barcodes for each species. Analysis of the COI gene sequences shows that barcode gap was distinct for between species, genera and at higher taxonomic levels. An increase in the mean K2P divergence across different taxonomic levels was observed. The mean intraspecific divergence was (0.7%). The mean interspecific divergence was (11.7%) and the mean divergence between genera was (21.1%).  The mean A+T contents were (66.3%). From the Neighbor Joining, Maximum Likelihood and Minimum Evolution clustering analyses, we concluded that DNA Barcoding is quite helpful for identification of Pentatomomorpha species of the area. This study encourages using DNA barcodes as complement with a morphological taxonomy for easy identification of plant bug species.


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