CO2 application as growth stimulator of sea grass, Thalassia hemprichii under laboratory conditions

Paper Details

Research Paper 01/12/2014
Views (187) Download (9)

CO2 application as growth stimulator of sea grass, Thalassia hemprichii under laboratory conditions

Khristin I.F Kondoy, Endang Yuli Herawati, Mohammad Mahmudi, Rodliyati Azrianingsih
J. Bio. Env. Sci.5( 6), 153-159, December 2014.
Certificate: JBES 2014 [Generate Certificate]


Seagrass study needs to be continuously developed to know its potential ability of storing carbon and absorbing CO2 (carbon sink) or called blue carbon used for photosynthesis. One of the studies uses carbondioxide (CO2) as growth rate indicator of the seagrass, Thalassia hemprichii, in a controlled laboratory scale. The seagrass used in this study was collected from Tongkaina waters, Sulawesi Utara. The study used Complete Randomized Design with treatments as follows: P1 – CO2 addition once per 3 days (5×100 ml/min for 25 minutes), P2 – CO2 addition once per 2 days (5×100 ml/min for 25 minutes), P3 – CO2 addition once a day (5×100 ml/min for 25 minutes), and control treatment (no carbondioxide). Results showed that daily growth rate was influenced by carbondioxide application and alkalinity. The highest wet weight occurred in P3 media in day-30, 36.67 gr, while the lowest wet weight was recorded in P1 in day-6, 27.60 gr. The highest growth rate was found in P3 in day-6, 1.310%, while the lowest was recorded in P1 in day-6, -1.772%. Carbondioxide application significantly affected the growth rate of the seagrass. Tukey test indicated that seagrass growth rate in P3 was significantly different from those of other treatments with P3>L: 0.747>0.65, while P1 and P2 did not have significant difference in growth rate with P1<L: 0.093<0.65 and P2<L: 0.068<0.65, respectively. Alkalinity measurement showed that the highest was recorded in P3, 80 mg/l and the lowest in P1, 40 mg/l.


Amiluddin, Masita N. 2007. Study on growth and carageenan content of seaweed, Kappaphycus alvarezii, infected with ice-ice disease in Pari waters, Seribu Islands. Thesis. Graduate School. Bogor Agriculture Institute, Bogor.

Anggadiredja A, Zatnika H, Purwoto, Istini S. 2007. Seaweeds Culture, processing, marketing of potential fisheries commodities. Penebar Swadaya, Jakarta. 146 p.

Cole GA. 1988. Textbook of Limnology. Third Edition. Waveland Press, Inc.,Illinois,USA. pp:401.

Duarte CM, Middelburg JJ, Caraco N. 2005. Major role of marine vegetation on the oceanic carbon cycle. Biogeosciences 2, 1-8.

Duarte CM, Middelburg Aminudin. 2008. Study on carbon reserve in public forest cultivation (case study: public forest of Dengok, Playen Playen District, Gunung Kidul Regency). Thesis. Bogor: Postgraduate School, Bogor Agriculture Institute.

Duarte CM, Kennedy H, Marba N, Hendriks I. 2011. Assesing the capacity of seagrass meadows for carbon burial: current limitations and future strategis. Ocean Coast Manag. In press.

Effendi H. 2003. Study on water quality for aquatic living resources management. Kanisius Yogyakarta.

Kennedy H, Beggins J, Duarte CM, Fourqurean JW, Holmer M, Marba N, Middelburg JJ. 2010. Seagrass sediments as a global carbon sink: Isotopic constraints, Global Biogeochem. Cycles 24 GB4026, doi:10.1029/2010 GB003848.

Kiswara W. 1992. Seagrasses in the reef flat of Pari island, Seribu islands, Jakarta. Oseanology in Indonesia. No. 25, 31-49.

Kiswara W, Ulumuddin YI. 2009. Role of coastal vegetation in global carbon cycle; mangrove and seagrass as carbon sinks. Workshop Ocean and climate change. Ocean as climate change control: the role of Indonesia sea in reducing the global warming process acceleration. Bogor, August 4th, 2009.

Kiswara W. 2010. Preliminary study: seagrass potential as carbon sink and absorption in Pari waters, Jakarta Bay. J. Indonesian Oseanology and Limnology di Indonesia.36(3),361-376.

Mackereth, Heron J, Talling JF. 1989.  Water Analysis. Freshwater Biological Association, Cumbria, UK.

Mattjik A, Sumertajaya M. 2002. Experimental designs with SAS Aplication and Minitab 1. 2nd ed. Bogor. IPB Press.

Mubarak H, Ilyas S, Ismail W, Wahyuni IS, Hartati ST, Pratiwi E, Jangkaru Z, Arifudin R. 1990. Technical guide to seaweed culture. Agricultural research and development board. Departement. Agriculture, Jakarta.

Nelleman, C. (ed.). 2009. Blue carbon: The role of healthy oceans in binding carbon. A rapid response asseement. United Nations Environment Programme. Norway.

Nelson DW, Sommers LE. 1996. Total carbon, organic carbon, and organic matter. In: Methods of Soil Analysis, Part 2, 2nd ed., A.L. Page et al., Ed. Agronomy. 9:961-1010. Am. Soc. of Agron., Inc. Madison, WI

Odum EP. 1971. Fundamental of Ecology. W.E Saunders, Philidelphia. 574p.

Siregar SA. 2005. Disposal water processing Installation. Kanisius. Yogyakarta. Indonesia.

Sulistijo, Atmadja WS. 1996. Seaweed culture development in Indonesia. Puslitbang Oseanografi LIPI. Jakarta, Indonesia.

Tomascik JW, Mah AJ, Nontji A, MOOSA MK. 1997. The Ecology Of The Indonesian Seas, Part Two. Periplus Edition.Canada.