Int. J. Biosci.10( 3), 283-287, March 2017
Barley Yellow Dwarf disease exclusively afflicts plant species in the family Poaceae and is especially noticeable where ever crops such as barley, maize, oats and wheat are cultivated. The total RNA extracted through TRI reagent and Reverse transcription Polymerase chain reaction (RT-PCR) was carried out by using Revert AidTM H-minus. For confirmation, Coat Protein Gene (CpG) specific primers of BYDV-PAV were used. The result showed that BYDV-PAV exhibit a high frequency of nucleotide and amino acid homogeneity within CpG region. As compared with inter population the isolate JQ811488 (from oat) found a unique thread of 3 amino acid difference at 3` “ANP” while isolate JQ811487 (from wheat) showed 9aa differences mostly at 5`. Pakistani isolate JQ811489 (from maize) found maximum 99.2% similarity with US isolate DQ285673 which is highest as compare with inter population. This study will increase understanding of the genetic diversity of Pakistani isolates of BYDV and their relationship among and with other isolates.
Brault V, Herrbach É, Reinbold C. 2007. Electron microscopy studies on luteovirid transmission by aphids. Micron 38(3), 302-312.
Elena S, Agudelo-Romero P, Carrasco P. 2008. Experimental evolution of plant RNA viruses. Heredity 100(5), 478-483.
Gibbs A, Gibbs M, Ohshima K, Garcia-Arenal F. 2008. More about plant virus evolution; past, present and future. Origin and evolution of viruses 229-250.
Gray S, Gildow FE. 2003. Luteovirus-Aphid Interactions*. Annual review of phytopathology 41(1), 539-566.
Hall G. 2006. Selective constraint and genetic differentiation in geographically distant barley yellow dwarf virus populations. Journal of general virology 87(10), 3067-3075.
Li C, Cox-Foster D, Gray SM, Gildow F. 2001. Vector specificity of barley yellow dwarf virus (BYDV) transmission: identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae. Virology 286(1), 125-133.
Lister RM, Ranieri R. 1995. Distribution and economic importance of barley yellow dwarf. Barley yellow dwarf 40, 29-53.
Mastari J, Lapierre H, Dessens JT. 1998. Asymmetrical distribution of barley yellow dwarf virus PAV variants between host plant species. Phytopathology 88(8), 818-821.
Mayo M, d’Arcy C. 1999. Family Luteoviridae: a reclassification of luteoviruses. The Luteoviridae 15-22.
Miller WA, Liu S, Beckett R. 2002. Barley yellow dwarf virus: Luteoviridae or Tombusviridae? Molecular Plant Pathology 3(4), 177-183.
Pagán I, Holmes EC. 2010. Long-term evolution of the Luteoviridae: time scale and mode of virus speciation. Journal of Virology 84(12), 6177-6187.
Saleem K, Hameed S, Ul-Haque I. 2013. Phylogenetic analysis of coat protein gene of BYDV-MAV strain from wheat. Archives of Phytopathology and Plant Protection 46(14), 1747-1755.
Stern A, Sorek R. 2011. The phage‐host arms race: shaping the evolution of microbes. Bioessays 33(1), 43-51.
Svanella-Dumas L, Candresse T, Hullé M, Marais A. 2013. Distribution of Barley yellow dwarf virus-PAV in the sub-Antarctic Kerguelen Islands and characterization of two new Luteovirus species. Plos one 8(6), e67231.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics nalysis version 6.0. Molecular biology and evolution 30(12), 2725-2729.