Comparative analyses of wheat leaf proteome under drought stress using 2D-PAGE
Paper Details
Comparative analyses of wheat leaf proteome under drought stress using 2D-PAGE
Abstract
Drought is one of the most important abiotic stresses throughout the world. Wheat as a major crop is mostly cultivated in area that encountered with drought stress at least in a period of year. Proteomics is one of the approaches to identify proteins involved in plant tolerance to drought stress. To study the effects of drought stress on wheat leaf proteome pattern in susceptible (Bahar) and tolerant (Kavir) cultivars of spring wheat; comparisons between drought stressed and control samples of both varieties was performed in terms of morpho-physiological traits in addition to proteome changes. Leaf proteins were extracted using TCA/aceton method and protein expression pattern was obtained using two-dimensional electrophoresis. Proteins involved in drought stress were identified by comparison of expression profile between drought stressed and control samples of both varieties. The results showed that there are significant differences between the treatments for almost all of the traits. The leaf proteome pattern analysis identified 13 protein spots in each of the comparisons (a total of 26 spots) representing a reproducible significant expression changes. The protein spots classified into functional groups include: photosynthesis, metabolic pathways, stress defense/response, photorespiration, protein synthesis/assembly and proteins with unknown functions. The reasonable effects and roles of identified proteins in drought stress were discussed. These results would help for better understanding of drought response molecular basis in plants to improve drought resistance in wheat.
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