home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Comparative analysis of genetic diversity among three species of the freshwater fish genus Garra (Osteichthys: Cyprinidae) using restriction fragment length polymorphism

Paper Details

Research Paper 01/03/2012
Views (553) Download (48)
current_issue_feature_image
publication_file

Comparative analysis of genetic diversity among three species of the freshwater fish genus Garra (Osteichthys: Cyprinidae) using restriction fragment length polymorphism

M Raja, P Malaiammal, S Nandagopal, M Muralidharan, M Arunachalam
Int. J. Biosci.2( 3), 88-96, March 2012.
Certificate: IJB 2012 [Generate Certificate]
Key: GarraGenetic diversityMeristicMorphometricRFLPWestern ghats

Abstract

The genetic variation in three species of the freshwater cyprinid Garra was studied using the traditional morphometric, meristic and Restriction fragment length polymorphism as molecular tool analysis. Samples were collected from their respective geographic locations of southern Western Ghats. Based on the 46 morphometric and 18 meristic characters employed during this study 23 characters showed variation among the three species and hence were utilized for the PCA ordination. The principal component analysis was performed using 15 morphometric and 8 meristic characters of which 12 components were extracted and the first three axes showed eigenvalues >1 and they explained the variance about 81.46 % of the total variance. The genome size of the species Garra mullya ranged from 3.8-6.15 µg/mg, Garra kalakadensis ranged from 3.25- 6.3 µg/mg and Garra gotyla stenorhynchus ranged from 3.9- 6.15 µg/mg. Based on the electrophorogram, different bands of fragments in each lane and band volume were analyzed, According to Hind III enzyme the electrophorogram analysis showed maximum fragment length polymorphism in Garra mullya which had four fragments and the total volume of bands was 12.582 nmoles. Based on the Eco R1 enzyme digestion the electrophorogram analysis revealed that the maximum fragment length polymorphism in Garra mullya composed of four fragments and the total volume of bands in the entire lane was 10.5965 nmoles. Based on the Hind III and Eco R1 restriction enzymes, the cluster analysis clearly showed that the Garra mullya and Garra kalakadensis grouped together while Garra gotyla stenorhynchus with distinct geneticdistance did not cluster with the other two species. Garra gotyla stenorhynchus can also be distinguished morphologically from Garra mullya and Garra kalakadensis.

VIEWS 41
Cover PDF

Arunachalam M, Muralidharan M. 2008. Cypriniform fishes of Peninsular India: Taxonomy and Generic descriptions. In: Arunachalam M, Ravichandran P, and Murugesan A G (ed.) Conservation Ecology. Publication Division, Manonmaniam Sundaranar University. P. 1-91.

Bossier P. 1999. Authentication of seafood products by DNA patterns. J. Food Sci. 64, 189–193.

Davidson WS. 1998. PCR techniques. In: Ashurst PR, Dennis MJ, eds. Analytical methods of food authentication. Blackie Academic & Professional.

Ferraris JD, Palumbi SR. 1996. Molecular Zoology: New York: Wiley-Liss (eds).

Hillis DM, Moritz C, Mable BK. 1996. Molecular Systematics, 2nd ed. Sinaver Assoc., Sonder land, Massachusetts.

Hubbs, Lagler. 1956. Fishes of the Great Lake Region. Bull. Grandbrook Inst. Sci. 26, 1-213.

Johnson JA, Rajesh RP, Arunachalam M. 2007. Comparative analysis of interpopulation genetic diversity in Puntius filamentosus using restriction fragment length polymorphism RFLP analysis, Afri. J. Biotech. Vol.6 (23) pp 2682- 2686.

Lockley AK, Bardsley RG. 2000. DNA-based methods for food authentication. Trends Food Sci Technol 11: 67–77. London: p. 182–203.

Nelson JS. 2006. Fishes of the World, Fourth edition. Wiley, New York.

Ponniah AG, Gopalakrishnan A. 2000. Cultivable ornamental, sport and food fishes endemic to peninsular India with special reference to Western Ghats In: Ponniah, A.G Gopalakrishnan A (eds.) endemic fishes diversity of Western Ghats. NBFGR-NATP publication, National Bureau of Fish Genetic Resources, Lucknow, India, p. 13-32

Sambrook  J,  Fritsch  EF,  Maniatis  T.  1989. Molecular Cloning, a laboratory manual, 2nd Edition. Gold Spring Harbor Laboratory Press. New York, 3, 18-55.

Takahashi D, Ohara K. 2004. Genetic variations estimated from PCR-RFLP analysis of two morphs of the freshwater goby Rhinogobius in the Lake Biwa water system. Ichthyol Res. 51, 99–105 DOI 10.1007/s10228-003-0199x.

Submit Manuscript