Comparative antibacterial activity of Aloe barbadensis miller and Azadirachta indica against bacterial isolates obtained from acne patients

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Research Paper 01/08/2020
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Comparative antibacterial activity of Aloe barbadensis miller and Azadirachta indica against bacterial isolates obtained from acne patients

Musarrat Sharif, Arif Malik, Mehwish Shaheen
Int. J. Biosci.17( 2), 36-57, August 2020.
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Propionibacterium acnes and Staphylococcus epidermidis are pus forming bacteria that trigger inflammation in acne while Staphylococcus aureus and Pseudomonas aeruginosa are the supportive agents for acne. The present study was conducted to compare the anti-acne activity of Azdirachta indica and Aloe barbadensis miller. Various extracts of Azadiracta indica and Aloe barbadensis miller were tested for antimicrobial activity by disc diffusion method and well diffusion method. It is concluded that Aloe barbadensis miller extracts has greater zone of inhibition against P. aeruginosa and S. aureus in comparison to Azadirachta indica extracts. On the other hand Aloe barbadensis miller extracts and Azadirachta indica extracts showed the maximum zone of inhibition against acne causing agents e.g. S. epidermidis and P. acnes. So, Azadirachta indica has better choice for anti-acne activity as compared to Aloe barbadensis miller.


Adegoke, Anthony A and Komolafe, Amos O. 2008. Nasal Colonization of School Children in Ile-Ife by Multiple Antibiotic Resistant Staphylococcus aureus. International Journal of Biotechnology and Allied Sciences 3, 317-322.

Adnan A, Ayaz MK. 2011. In vitro bactericidal and bacteriostatic potential of ingredients of traditional medicine obtained from kacha area (river indus) district Dera Ismail Khan (D.I. KhanDistrict, Khyber Pakhtun Khwa (KPK), against human bacterial pathogens. Journal of Botany 5, 2613- 2617.

Agarry, Olaleye MT, Bello M. 2005. Comparative antimicrobial activities of Aloe vera gel and leaf.  Journal of biotechnology 4, 1413- 1414.

Al-Emran A, Ahmed SM, Farzana, Saha, Sajal KD, Sreedam C, Bachar SC. 2011. Evaluation of Brine shrimp lethality and Antimicrobial activity of Azadirachta indica leaf extract on some drug resistance bacteria in Bangladesh. Pharmacognosy Journal 3, 66-71.

Autade RH, Saini S, Reddy PG, Deorukhkar SC, Padmajakshi G. 2015. Effect of Azadirachta indica bacterial and fungal pathogens associated with AIDS. Journal of Current Microbiology extract against opportunistic and Applied Sciences 4, 988- 999.

Ayandele AA, Adeniyi SA. 2011. Prevalence and antimicrobial resistance pattern of microorganisms isolated from Naira notes in Ogbomoso North, Nigeria. Journal of Research in Biology 8, 587-593.

Bachoon D, Dustman, Wendy A. 2008. Selective and differential media for isolation” Journal of Microbiology Laboratory Manual. Mason 175- 176.

Boni R, Nehrhoff B. 2003. Treatment of gram-negative folliculitis in patients with acne” Journal of Clinical Dermatology 4, 273- 276.

Boost MVO, Donoghue MMJ. 2008. Prevalence of Staphylococcus aureus carriage among dogs and their owners. Journal of Epidemiology Infection 7, 953- 964.

Brisse S, Verhoef. 2000. Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella  oxitoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and genes sequencing and automated ribotyping. International Journal of Systematic and Evolutionary Microbiology 51, 915- 924.

Cenci-Goga BT, Karama M, Rossitto PV, Morgante RA, Cullor. 2003. Enterotoxin production by Staphylococcus aureus isolated from mastitis cows. Journal of food protection 669, 1693- 1696.

Chinnaperumal K, Abdul R, Chinnadurai S, Moorthy L,  Arivarasan VK. 2012. Evaluation of antibacterial activity of selected medicinal plant extracts from south India against human pathogens. Journal of Tropical Disease 296- 301.

Cosgrove SE, Vigliani GA, Campion. 2009. Initial low-dose gentamicin for Staphylococcus aureus bacteremia and endocarditis is nephrotoxic.  Journal of Clinical Infectious Disease 486, 713- 721.

Costerton WA. 2014. The Microbe and Pathogen, Pseudomonas aeruginosa Infections and Treatment. 1- 17.

Esra MMA, Aisha A, Salwa M, Khogali E, Umel Kheir MA, Gergeir. 2012. Antimicrobial activity of Cannabis sativa L. Journal of Scientific Research 3, 61- 64.

Farhat S, Daud G, Pande M, Joshi R, Pathak S, Wankhede. 2013. A study of antibacterial effect of some selected essential oils and medicinal herbs against Acne Causing Bacteria. Journal of Pharmaceutical Science 2, 27- 34.

Friedlander. 1882. Uber dies scizomyceten bei der acuten fibrosen pneumonie. Journal of Archieve Pathology Anatomy Physiology Klin Medicine 87, 319- 324.

Hota B. 2004. Contamination, disinfection, and cross-colonization: Are Hospital surfaces reservoirs for nosocomial infection. Clinical Infectious Disease 39, 1182- 1189.

Kalpesh KP, Neel JM, Milan CD, Alkesh KB, Divyesh HS, Pragna KS, Gaurang BS. 2012. Development and Evaluation of Herbal Anti-Acne Formulation. Research Journal of Pharmaceutical, Biological and Chemical Sciences 3, 334.

Korzeniowski O, Sande. 1982. Combination antimicrobial therapy for Staphylococcus aureus endocarditis in patients addicted to parenteral drugs and in non addicts a   prospective study. Journal of Annals Internal Medicine 974, 496- 503.

Krieg, Noel, Bergey’s. “Manual of Systematic Bacteriology” 1984(1), 638- 649.

Kumar GS, Jayaveera KN, Ashok CK, Kumar,  Umachigi P, Sanjay BM, Vrushabendra Swamy DV, Kishore K. 2007. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Journal of Pharmaceutical Research 6, 717- 723.

Lalitha DD, Srinivas B, Bandaru NR. 2012. An evaluation Antimicrobial activity of Aloe barbadensis Miller Gel Extract. Journal of pharmaceutical and biomedical sciences 21, 23- 63.

Levine M. 1918. Differentiation of E. coli and E. aerogenes on a simplified eosin-methylene blue agar. Journal of Infectious Disease 23, 43- 47.

Mack D, Davies A, Harris L, Rohde H, Horstkotte M, Knobloch. 2007. Microbial interactions in Staphylococcus epidermidis biofilms. Journal of Analytical and bioanalytical chemistry 387, 399- 408.

Muddu S, Chamalla SK, Nitin M, Konuri S, Indugula JP. 2015. Bacteriology of Acne. Journal of Evolution of Medical and Dental Sciences 4, 3267-3274.

Muna JA, Rasha FO, Rana FO. 2019. Antibacterial Activity for Acne Treatment through Medicinal Plants Extracts: Novel Alternative Therapies for Acne. Journal of Pure and Applied Microbiology 13, 1245-1250.

Murali S, Jambulingam M, Sen S, Jeyavel P, Priya K, Lily T, Hajib N, Madhavan. 2015.  A study on the characterization of Propionibacterium acnes isolated from ocular clinical specimens. Indian Journal of Medicine Research 142(4), 438- 449.

Neel R, Ragini D. 2012. Capsular typing of coagulase positive (COPS) community associated Methicillin Resistant Staphylococcus aureus (CAMRSA) isolated from anterior nares of school children from Lushoto, Korogwe, Muheza and Tanga districts in Tanzania. Journal of Pharmacophore 32, 117- 122.

Nikunj B, Gurjar. 2012. Development and evaluation of cinnamon and Aloe barbadensis miller containing herbal anti-acne gel. Indian Journal of pharmacy 2, 446-451.

Parisi J. 1985. Coagulase-Negative Staphylococci and the epidemiological typing of Staphylococcus epidermidis. Journal of Microbiological Reviews 49, 126-139.

Presterl E, Suchomel M, Eder M, Reichmann S, Lassnigg A, Graninger W, Rotter M. 2007. Effects of alcohols, povidone-iodine and hydrogen peroxide on biofilms of Staphylococcus epidermdis. Journal of Antimicrobial Chemotherapy 60, 417- 420.

Priyam S, Shruti S, Nidhi M, Narayan PY. 2014. New perspectives on anti-acne plant drugs. International Journal of Biological Medicine Research 14, 1- 19.

Puguh R, Prasetyowati S. 2015. Effect of soy isoflavones on Acne vulgaris. Journal of Pakistan association of dermatologists 1, 30- 34.

Rahul S, Abhishek P, Mamta S, Bela P. 2015. Anti-acne activity of Achillea Moonshine petroleum ether extract. Journal of Medicine Plants Research 9(27), 755- 763.

Renisheya J, Jeba M, Johnson N, Nancy B, Laju A, Renola J, Jeba E. 2012. Anti-bacterial and antifungal activity of Aloe barbadensis miller gel extract. International Journal of Biomedical and Advance Research 03, 42- 78.

Rubina L, Priyanka T, Ebenezer J. 2009. Isolation, purification and evaluation of antibacterial agents from Aloe vera. Brazilian Journal of Microbiology 40, 906-915.

Ryan K, Ray J. 2017. An Introduction to Infectious Disease Journal of Sherris Medical Microbiology 4, 294- 295.

Saba I, Muneeba B, Hira Y. 2011. In-Vitro antibacterial activity of Aloe barbadensis Miller. Journal of pharmaceutics 1, 59- 64.

Sabahat S, Perween T. 2008. In vitro antibacterial activity of clove against gram negative bacteria. Pakistan Journal of Botany 8, 2157- 2160.

Sulakvelidze A, Alavidze Z, Glenn M. 2001. Bacteriophage therapy antimicrobial agents. Chemotherapy 45, 649- 659.

Thiruppathi S, Ramasubramanian V, Sivakumar T, Thirumalai A. 2010. Antimicrobial activity of Aloe barbadensis miller (L.) against pathogenic microorganisms. Journal of Biological Sciences Research 1, 251- 258.

Uraku. 2016. GC/MS Determination of bioactive constituents of methanol fraction of Spilanthes uliginosa (Sw) leaves. Journal of Medicinal Plant 1819- 3455.

Wen CK, David LP, Anthanasia J, Sagnimeni DS, Hansen AV, Sunita M, Jose MC, Herman G, Lutfiy M, Gordon T, Keith K, Joseph G. McCormack VLY. 2002. Community-Acquired Klebsiella pneumoniae bacteremia global differences in clinical patterns. Emerging infectious diseases 8, 2.

Zainab A, Ghani C, Alaa H, Al-Charrakh, Nada K, Hindi K, Shatha K, Kadhim H. 2013. Antimicrobial effect of aqueous banana peel extract. Journal of Pharmaceutical Sciences 1, 73- 75.

Zohreh H, Majid S, Katayoun MS, Aida S. 2014. Effect of Aloe vera Tropical gel combined with tretinoin in treatment of mild and moderate Acne vulgaris: a randomized, double-blind, prospective trial. J. Dermatological Treatment 25, 123- 129.