Int. J. Biosci.15( 4), 40-51, October 2019
The present study objective was to evaluate and compare the utmost antifungal activity by screening different parts of Eucalyptus torelliana F. Muell. (Cadaghi) have been successively extracted in a variety of solvents against most hostile isolate of Bipolaris sorokiniana Sacc. In-vitro bioassay conducted with crude extracts at different concentrations (1%, 05% & 10%) for radial mycelial growth, inhibition zone and biomass production of B. sorokiniana. Inhibition of pathogen was check by food poison technique and well diffusion assay in seeded agar plates. Colony growth inhibition due to leaves extracted in ethanol (76%) and methanol components of flower buds (75%) had been found to get higher than both ethanol and methanol extracts of bark (58%). Likewise for aqueous ingredients bark and leaf proved higher inhibition (52% & 50%) compare to flower buds. The inhibition zone observed for flowering buds were 29.15±0.88; 27.40±1.25; 26.15±1.03 and 0.00±0.00mm at highest concentration (5.3mg/100μl) for methanol, ethanol, aqueous and control treatments respectively against B. sorokiniana. The highest decrease found in hyphae fresh and dry weight (0.026 & 0.02g) treated with flower bud methanol extract in contrast of aqueous extracts (2.28 & 2.23g). Length/width of extract treated conidia (30±2.88/20±1.91μm) and conidiophores (111±16.42/5.50±0.56μm) have been significantly decreased with respect of control treated conidia 77±0.54/25±0.15μm and conidiophores 141±1.69/7.33±0.44μm. The average numbers of septa within treated conidia were basically 2-6 in control 2-7 had been observed. Really small variations were seen in colony color, margin, texture and hyphae thickness in extract treated and control treatments.
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