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Comparative evaluation of Bacillus thuringiensis and Bacillus sphaericus against Dengue fever vector Aedes albopictus larvae, pupae emergence and adult inhibition in laboratory in Lahore, Pakistan

Research Paper | July 1, 2017

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Wajeeha Hameed, Dr. Nusrat Jahan, Ferhat Mehmood

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J. Bio. Env. Sci.11( 1), 56-62, July 2017


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Dengue is a growing public health problem in many tropical and subtropical countries worldwide. At present the only method of controlling or preventing the disease is to eliminate its vectors viz Aedes aegypti and Aedes albopictus. In current study, susceptibility of wild caught laboratory reared Aedes albopictus early 4th instar larvae against, Bti TP vectobac® 5000 ITU/mg, Bti WDG vectobac® 3000 ITU/mg and Bacillus sphaericus (Bsph) Vectolex® (TP) 1380 ITU/mg was evaluated. Lethal concentrations LC50-LC95 ranged between 0.26-1.21 and 0.047-0.28 respectively, against Bti TP, while 0.052 – 0.14 and 0.025-0.091 against Bti WDG and 0.44 -1.65 and 0.37-1.5 against Bsph at 24 and 48 hours post treatment. It indicated that early 4th instar larvae of Aedes albopictus were most susceptible against Bti WDG and least susceptible against Bsp in laboratory. Comparative probit regression analysis indicated that Bti WDG was highly toxic at 48 and 72 hours exposure at the same mortalities. Pupae emergence was completely inhibited at 1 ppm and > 50% at 0.01 ppm against Bti TP in laboratory. Mean percent mortalities were 81±3.53, 53±3.53, 37±2.31, 25±3.53 and 16±2.31 against 1, 0.1, 0.01, 0.001 and 0.0001 ppm at 24-72 hours post exposure respectively, with significant difference between Bti TP and Bsph at P < 0.05.


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Comparative evaluation of Bacillus thuringiensis and Bacillus sphaericus against Dengue fever vector Aedes albopictus larvae, pupae emergence and adult inhibition in laboratory in Lahore, Pakistan

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