Comparative evaluation of Bacillus thuringiensis and Bacillus sphaericus against Dengue fever vector Aedes albopictus larvae, pupae emergence and adult inhibition in laboratory in Lahore, Pakistan

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Research Paper 01/07/2017
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Comparative evaluation of Bacillus thuringiensis and Bacillus sphaericus against Dengue fever vector Aedes albopictus larvae, pupae emergence and adult inhibition in laboratory in Lahore, Pakistan

Wajeeha Hameed, Dr. Nusrat Jahan, Ferhat Mehmood
J. Bio. Env. Sci.11( 1), 56-62, July 2017.
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Abstract

Dengue is a growing public health problem in many tropical and subtropical countries worldwide. At present the only method of controlling or preventing the disease is to eliminate its vectors viz Aedes aegypti and Aedes albopictus. In current study, susceptibility of wild caught laboratory reared Aedes albopictus early 4th instar larvae against, Bti TP vectobac® 5000 ITU/mg, Bti WDG vectobac® 3000 ITU/mg and Bacillus sphaericus (Bsph) Vectolex® (TP) 1380 ITU/mg was evaluated. Lethal concentrations LC50-LC95 ranged between 0.26-1.21 and 0.047-0.28 respectively, against Bti TP, while 0.052 – 0.14 and 0.025-0.091 against Bti WDG and 0.44 -1.65 and 0.37-1.5 against Bsph at 24 and 48 hours post treatment. It indicated that early 4th instar larvae of Aedes albopictus were most susceptible against Bti WDG and least susceptible against Bsp in laboratory. Comparative probit regression analysis indicated that Bti WDG was highly toxic at 48 and 72 hours exposure at the same mortalities. Pupae emergence was completely inhibited at 1 ppm and > 50% at 0.01 ppm against Bti TP in laboratory. Mean percent mortalities were 81±3.53, 53±3.53, 37±2.31, 25±3.53 and 16±2.31 against 1, 0.1, 0.01, 0.001 and 0.0001 ppm at 24-72 hours post exposure respectively, with significant difference between Bti TP and Bsph at P < 0.05.

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Barbazan P, Baldet T, Darriet F, Escaffre H, Haman D, Hougard JM. 1997. Control of Culex quinquefasciatus (Diptera: Culicidae) with Bacillus sphaericus in Maroua, Cameroon. J Am Mosq Control Assoc 13, 263-269.

Baumann GGeisse SSullivan M. 1991. Cyclosporin A and FK-506 both affect DNA binding of regulatory nuclear proteins to the human interleukin-2 promoter. New Biol. 3(3), 270-8.

Becker N.1992. Community participation in the operational use of microbial control agents in mosquito control programmes. Bull Soc Vector Ecol 17, 114-118.

Chan SY, Kautner IM, Lam SK. 1995. Detection and serotyping of dengue viruses by PCR: a simple rapid method for the isolation of viral RNA infected mosquito’s larvae. J. Trop. Med. Public Health 25, 258-261.

Chareonviriyaphap T, Akratanakul P, Nettanomsak S. 2003. Larval habitats and distribution patterns of Aedes aegypti (Linnaeus) and Aedes albopictus (skuse) in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 34, 529-535.

Charles JF, Nielsen-Le-Roux C. 2002. Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena. Memories Do Institu to Oswaldo Cruz 95, 201-206.

Chow-Shaffer E, Sina B, Hawley WA. 2000. Laboratory and field evaluation of polymerase chain reaction based forensic DNA profiling for use in identification of human blood meal sources of Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 37(4), 492-502.

Eritga R, Escosa R, Lucientes J, Marques E, Roiz D, Ruiz S. 2005. Worldwide invasion of mosquito’s present European distribution and challenges for Spain. Biological Invasion 7(1), 87-97.

Fillinger U, Knols BGJ, Becker N. 2003. Efficacy and efficiency of new Bacillus thuringiensis  var. israelensis and Bacillus sphaericus formulations against Afrotropicalanophelines in Western Kenya. Trop Med Int Health 8, 37-47.

Guha-Sapir DM, Schimmert B. 2005. Dengue fever: new paradigms for a changing epidemiology. Emerging themes in epidemiology. Open access journal.

Kosiyachinda P, Bhumiratana A, Kittayapong P. 2003. Enhancement of the efficacy of a combination of Mesocyclops aspericornis and Bacillus thuringiensis var. israelensis by community-based products in controlling Aedes aegypti larvae in Thailand. The American journal of tropical medicine and hygiene 69(2), 206-12.

Mittal CJ, Adak T, Batra CP. 2001. Comparative toxicity of selected larvicidal formulations against Anopheles stephensi Liston and Aedes aegypti. Linn. J. Commun 33(2), 116-120.

Pratt HD, Moore CG. 1993. Mosquitoes of public health importance. Eco Access Publications 58-71.

Tewari SC, Munirathinam A, Ganjanana A. 2004. Dengue vector prevalence and viral infection in a rural area in South India. Trop. Med. Int. Health 4, 499-507.

World Health Organization. 1999. International program on chemical safety (IPCS): microbial pest control agent Bacillus thuringiensis. Environmental Criteria 217, 1-105.