Comparison of genetic diversity among Medicago ciliaris populations prospected from highly salted region (Oran Great Sebkha) using morphological pod descriptors of IBPGR and SDS-PAGE markers

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Research Paper 01/12/2017
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Comparison of genetic diversity among Medicago ciliaris populations prospected from highly salted region (Oran Great Sebkha) using morphological pod descriptors of IBPGR and SDS-PAGE markers

Djilali Moulai, Fatima Zohra Fyad-Lameche
Int. J. Biosci.11( 6), 27-38, December 2017.
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With the aim of characterizing and evaluating spontaneous M. ciliarisspecies of annual Medicago genus which are adapted to salty conditions. A comparison was realized on 11 populations among which seven are prospected near and far from the Great Sebkha of Oran while four others are used as a reference. This study is pressed on two approaches, the one is based on the biometrics of the morphological traits of pods using IBPGR descriptors, and the other one is a biochemical approach using the one-dimensional sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) of the seed storage proteins. The biometric results showed a higher level of significance (p < 0.0001) for the almost characters, but not absolutely significant at (p > 0.05) for the number of coils NC, number of seeds per pod (NS) and for pod height (H). Accordingly, the nearest population from the Sebkha has a dwarfish aspect of pods than the others populations. The SDS-PAGE of total and globulin proteins revealed only minor differences between populations in the number, density, and intensity of polypeptide bands. So we found two distinctive polypeptide bands were observed for globulin and total protein gel, respectively; the first has~80kDa and it’s specific to the nearest population. The second is missing from it and has ~68 kDa. Itappears that the combination of morphological pod traits and SDS-PAGE markers are useful tools for a preliminary investigation of genetic variation of Medicago ciliaris. This study helped us to determine the population that has adapted to salty conditions.


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