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Research Paper | September 1, 2019

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Comparison of methods of inoculation for antibacterial potential assay

Shazia Yaqub, Mian Anjum Murtaza, Shinawar Waseem Ali, Abdul Munim Farooq

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Int. J. Biosci.15(3), 328-333, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.328-333

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Abstract

Two different methods of inoculations (spreading and pouring) were compared to choose one for better results for antibacterial potential assay. For comparison, two different parameters were selected that includes inoculation method and quantity of supernatant. In spite of spreading variable quantities of indicator organisms, no remarkable difference was observed on growth bed on agar media after incubation. When indicator organisms were poured after mixing with luke warm media, significant difference in growth bed was observed. The quantity of supernatant of test isolate P15 (Bacillus leicheniformis) showed a direct relation to inhibition zone formed. Suggesting that pouring method is effective to test the antibacterial potential of isolated strains against indicator organisms by agar well diffusion method.

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Comparison of methods of inoculation for antibacterial potential assay

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