Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.)
Paper Details
Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.)
Abstract
Weed dodder is a perennial herb belonging to the Convolvulaceae family and is widely used as a valuable herbal drug in traditional medicine. In this study, ISSR and RAPD markers were used to evaluation of genetic diversity of 20 genotypes of weed dodder (Cuscuta epithymum L.) from Golestan and Mazandarn provinces. 6 ISSR primers amplified 90 bands that 76.33% bands were polymorphism. Average polymorphism information content (PIC) and average marker index value was 0.26 and 20 for ISSR markers respectively. RAPD markers amplified 195 bands that more than 95% bands were polymorphism. Average PIC and average MI was 0.27 and 24.7 for this markers calculated. Cluster analysis based on DICE coefficient and UPGMA algorithm showed that there is wide variation in samples collected. The average of similarity based on ISSR markers was 0.57 and average of similarity based on RAPD markers was 0.45. This high level of diversity could be related to the existents of several sub species in weed dodder (Cuscuta epithymum L.) and the high discrimination power of molecular markers that be used in this study.
Doyle J. 1991. DNA protocols for plants – CTAB total DNA isolation. In: Hewitt GM, Johnston A (Eds.) Molecular Techniques in Taxonomy. Springer, Berlin.
Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. African journal. 19: 11-15.
Fasih Z, Farshafvar M, Safari H. 2013. Genetic diversity evaluation of within and between population for Festuca arundinacea by ISSR markers. International Journal of Agriculture and Crop Sciences 5(13): 1468-1472.
Gustine DL, Voigt PW, Brummer E, Papadopuolos A. 2002. Genetic variation of RAPD markers for North American white clover collections and cultivars. Crop Science 42: 343- 347.
Khan K, Prasad MN. 2010. Optimization of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India. African Journal of Biotechnology 5 (3): 230-234.
Mahmood MK, Luikart G, Waples RS. 1997. Genetic monitoring as a promising tool for conservation and management. Molecular journal sciences 22: 25–33.
Mclelland DL, Voigt PW, Brummer E, Papadopuolos A. 1995. Genetic variation of RAPD markers for North American white clover collections and cultivars. Crop Science 42: 343- 347.
Me J, Heydarinezhad AM, Niridehghan M. 1999. The specialist Hemiptera associated with Mistletoe. British Journal of Entomology and Natural History 12: 237-238.
Mejnartowicz L. 2004. Genetic Analysis of Silver-fir population in the North Carpathian and Sudeten Mountains. Turkish Society science 65 (3-4): 319-328.
Nei M. 1972. Genetic distance between populations. Am. Nature 106: 283-292.
Paeker H. 1993. European dodder – taxonomy, host trees, parts used, physiology. in. Bussing. Amsterdam: Harwood Academic Publishers 16: 120-235.
Palet R, Smouse PE. 2006. GenAlex 6: Genetic analysis in excel. population genetic software for Teaching and research. Molecular Ecology Notes 6: 288-295.
Parker H, Riches K. 2008. Botany of European dodder (Cuscuta sp). Oncology 43: 2–7.
Paudit, H. 1386. Iranian culinary culture. 1th. Publication of Agricultural Sciences. 4: 103-102.
Peakle A, Smouse M. 2006. Apoptosis -inducing properties of Cuscuta epithymum L., correlate with their content of toxic dodder. Biotech Research 9: 203-208.
Sadeghi Alikelayeh A, Fakheri B, Kazemitabar SK, Gholinezhad R. 2012. Optimization of thermo cycler set to determine of genetic diversity on weed dodder. Chalous journal of azad Islamic university of iran. 23: (7).
Sandler J, Sadeghi A, Farster J. 2001. Weed dodder – distribution, biology and the national survey. British Wildlife 7(2): 25-28.
Uddin H, Ghoiyd K, Berahmani S. 2007. Dodder lectins and their A and B chains. Oncology 43: 23-34.
Seyed Kamal Kazemitabar, Simin Azoush, Amin Sadeghi Alikelayeh, 2014. Comparison of RAPD and ISSR molecular markers to determine of genetic diversity in weed dodder (Cuscuta epithymum L.). J. Biodiv. Environ. Sci., 4(4), 19-26.
Copyright © 2014 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.