Comparison of the results of in vitro fertility cycles between cryopreservation method by vitrification oocytes and fresh oocytes in human

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Research Paper 03/01/2023
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Comparison of the results of in vitro fertility cycles between cryopreservation method by vitrification oocytes and fresh oocytes in human

Truong Quoc Thinh, Tu Nguyen Huynh Cam, Khoi Tran Vy, An Vo Thien, Huyen Nguyen Thi Thuong
Int. J. Biosci.22( 1), 53-62, January 2023.
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Abstract

Oocyte cryopreservation addresses several difficulties, such as maintaining female fertility, oocyte accumulation in women with decreased ovarian reserve, inadequate ovarian stimulation response, or developed “oocyte donation bank”. A retrospective and prospective cohort study were conducted at the Infertility Department – Hanh Phuc International Hospital, recording the results of 210 couples undergoing IVF cycles, with the goal of comparing and evaluating the effectiveness of treatment in two groups using frozen-thawed oocytes and fresh oocytes in IVF cycles (105 cycles using thawed oocytes and 105 cycles using fresh oocytes). Intracytoplasmic sperm injection was conducted on all mature oocytes from fresh and thawed oocytes (frozen and thawed by vitrification technique) (ICSI). The embryological and clinical results of the two groups were documented, compared, and validated using the t-test. Survival rate of the freezing – thawing process oocytes was 92.61% and there was no difference in the embryological and clinical outcome results between thawed and fresh oocytes, through the following criteria: the rate of fertilized oocytes (69.04% vs 73.43%, p = 0.1026) with good embryo (29.10% vs 33.87%, p = 0.1794), the rate of blastocyst formation (45.61% vs 56.55%, p = 0.0541). The cumulative pregnancy rate, clinical pregnancy rate, live birth rate was 47.17%, 40.75% and 35.51%, respectively, while group using thawed oocytes was 40.79%, 50.03% and 34.76%, respectively (p = 0.3176 – 0.2377 – 0.9067). The miscarriage rates in the two groups were 6.04% and 5.51% (p = 0.7615).

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