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Comparison of Zielh-Neelsen staining and polymerase chain reaction for the diagnosis of Johne’s disease in small ruminants in the district Lahore, Pakistan

Saba Badar, Ashiq Ali, Zafar Iqbal Choudary, Abdul Jabbar, Sidra Altaf, Qaisar Tanveer, Iqra Asif, Asima Tariq, Muhammad Saif Ur Rehman Babar, Muhammad Umar Amin, Muhammad Kashif, Akasha Tanveer, Kiran Choudhary, Sidra Aslam, Tehreem Umar

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Int. J. Biosci.18(5), 7-13, May 2021

DOI: http://dx.doi.org/10.12692/ijb/18.5.7-13


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Paratuberculosis is a bacterial disease that affects small and large ruminants and has an impact on the economy of the country in terms of production losses and cost of treatment and control. The objectives of this study were the diagnosis of Paratuberculosis or Johne’s disease (JD) in goats of Lahore, Pakistan and a comparison of the two diagnostic techniques for Paratuberculosis. Part of the small intestines and associated mesenteric lymph nodes were collected randomly from goats of all ages and both sexes. Those samples were subjected to the Herrold’s Egg Yolk Medium (HEYM) culture and DNA extraction followed by PCR and Ziehl-Neelsen’s staining. Acid-fast bacilli were found in the tissues that showed the gross lesions of severe enteritis and lymphadenitis in the collected samples. Results showed that there was a clinical and subclinical infection. This study showed PCR more sensitive in the detection of Insertion Sequence (IS) 900 genomic fragment as compared to the HEYM culture.


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Comparison of Zielh-Neelsen staining and polymerase chain reaction for the diagnosis of Johne’s disease in small ruminants in the district Lahore, Pakistan

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