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Complement fixation analysis for seroepidemiology of foot-and-mouth disease in District Muzaffargarh

Research Paper | August 1, 2018

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Sheifa Ejaz, Sajjad Ur Rahman, Ahsan Naveed, Mehboob Ahmad, Ghazal Ejaz

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Int. J. Biosci.13( 2), 154-159, August 2018

DOI: http://dx.doi.org/10.12692/ijb/13.2.154-159


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Foot-and-Mouth Disease (FMD) is considered as most colossal due to huge economic losses caused by the disease. It affects majority of cloven-footed animals including buffaloes, cattle, sheep, goats, camels, wild animals. District Muzaffar-Garh is known as one of the most important districts in Punjab, Pakistan, with significant livestock population. The data regarding its epidemiological occurrence is insufficient at the field level. The study was designed to investigate a cross-sectional survey to determine the seroprevalence of FMDV in selected areas of district Muzaffargarh. The sampling was carried out and completed through multistage random sampling for the seroprevalence. Blood samples and epithelial samples were collected from clinically affected animals. For sera preparation, the blood samples were collected in vacutainer tubes. The sera were examined for the complement fixation test (CFT). Epithelial tissues and oropharyngeal fluids samples of clinically positive cases were collected and processed. A total of 200 samples (cow and buffalo 100 from each) were collected and processed for CFT. The current data clearly revealed that the percentage of FMD positive sample in cow (76%) was comparatively higher as compared to a percentage (68%) in buffalo (but statistically the ratio of positive samples in cow and buffalo was non-significant (P>0.05). The data showed that 68 samples out of total 100 samples were positive for buffalo and 76 samples out of total 100 samples were positive for the cow.


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Complement fixation analysis for seroepidemiology of foot-and-mouth disease in District Muzaffargarh

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