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Composition of essential oil of Ocimum basilicum L., minimum and variability in antioxidant activity of essential oil of leaves and flowering tops of Ocimum basilicum L. Genovese following seasons of culture under arid climate (southeast of Algeria)

Nabila Slougui, AhlamTlili, Rokia Hammoudi, HadjerBentayeb, Mahfoud Hadj Mahammed

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Int. J. Biosci.12(4), 360-372, April 2018

DOI: http://dx.doi.org/10.12692/ijb/12.4.360-372


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In this work we have studied the antioxidant activity of essential oils from leaves and flowering tops of Ocimum basilicum L., genovese (Lamiacea) from three different seasons, all were cultivated in southeast of Algeria (Ouargla) in a Saharan climate. According to the results obtained from the scavenger effect test of the DPPH radical, the essential oils tested have a significant antiradical potential especially for the essential oils of the flowers and autumn leaves with a IC50 of 19.696 ± 0.01 and 20.536 ± 0.03 μg/ml respectively. The study of the composition of the essential oil of Ocimumbasilicum L. minimum, by GC and GC-MS in FULLSCAN mode as well as in the SIM mode has allowed the identification of the chemotype linalool with a percentage of 43.5%, 86 compounds were also identified representing 98.7% of the oil composition.


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Composition of essential oil of Ocimum basilicum L., minimum and variability in antioxidant activity of essential oil of leaves and flowering tops of Ocimum basilicum L. Genovese following seasons of culture under arid climate (southeast of Algeria)

Aburigal YAA, Hamza NB, Hussein IH, Elmogtaba EY, Osman TH, Ali F I, Siribel AA M. 2016. Variability in Content and Chemical Constituents of Essential Oil of Sweet Basil (Ocimumbasilicum L.) Obtained from Aerial Plant Parts. Sudan, Advances in Bioscience and Biotechnology, Scientific Research 7, 183-187 http://dx.doi.org/10.4236/abb.2016.73016

Adams RP. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th Edition, Allured Publishing Corporation, U.S.A.

Avetisyan A, Markosian A, Petrosyan M, Sahakyan N, Babayan A, Aloyan S and Trchounian A. 2017. Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars. BMC Complement Altern Med 17, 1-8. http://dx.doi.org/10.1186/s12906-017-1587-5

Beatovic D, Krstic-Milosevic D, Trifunovic S, Siljegivic J, Glamoclija J, Ristic M, Jelacic S. 2015. Chemical composition, antioxydant and antimicrobial activities of the essential oils of twelve Ocimum basilicum L., cultivars grown in Serbia. Records of natural products 9, 62-75.

Benzid  A, Slougui N, Hadj-Mahammed M. 2017. Etude comparative de l’activité antioxydante d’Ocimum basilicum L., gratissimum cultivé dans plusieurs régions d’Algérie. Third International Congress of SAN, Constantine (Algeria) P132, 28-30 novembre 2017 – CI-SAN 2017.

Bourkhiss M, Hnach M, Bourkhiss B, Ouhssine M, Chaouch A, Satran B. 2009. Effet de séchage sur la teneur et la composition chimique des huiles essentielles de Tetraclinisarticulata (Vahl) Masters. Productions végétales 20, 44-48.

Boutabia L, Telailia S, Bouguetof I, Guenadil F.et Chefrour A. 2016. Composition chimique et activité antibactérienne des huiles essentielles de Rosmarinus officinalis L. de la région de Hammamet (Tébessa-Algérie). Bulletin de la Société Royale des Sciences de Liège 85, 174-189.

Brada M, Hadj-Khlifa L, Achour D, Wathelet JP, Lognay G. 2012. Essential oil composition of Ocimum basilicum L. and Ocimum gratissimum L., from Algeria. Journal of Essential Oil Bearing Plants 14, 810-814.

Chalchat JC, Özcan M. 2008. Comparative essential oil composition of flowers, leaves and stems of basil (Ocimum basilicum L.) used as herb.in Turkey. Food Chemistry.110, 501-503. http://dx.doi.org/10.1016/j.foodchem.2008.02.018

Dolatabad SS, Moghaddam M, Chalajour H. 2014. Essential oil composition of four Ocimum species and varieties growing in Iran. Journal of Essential Oil Research 26, 315-321.

Eriotou E, Anastasiadou K, Nikolopoulos D, Koulougliotis D. 2015.Antimicrobial and Free Radical Scavenging Activities of Basil (Ocimum basilicum) Essential Oil Isolated from Five Plant Varieties Growing in Greece. J Nutr Food Sci 5, 367-371.

Espín JC, Soler-Rivas C, Wichers HJ. 2000. Characterization of the Total Free Radical Scavenger Capacity of Vegetable Oils and Oil Fractions Using 2, 2-Diphenyl-1-picrylhydrazyl. Journal of Agricultural and Foof Chemistry 48, 648-656. http://dx.doi.org/10.1021/jf9908188

Filipa S, Vidovića E, Vladića J, Pavlića B, Adamovićb D, Zekovića Z. 2016. Chemical composition and antioxidant properties of Ocimum basilicum L. extracts obtained by supercritical carbon dioxide extraction: Drug exhausting method. The Journal of Supercritical Fluids 109, 20-25. http://dx.doi.org/10.1016/j.supflu.2015.11.006

HadjKhelifa L, Brada M, Brahmi F, Achour D, Fauconnier ML, Lognay G. 2012. Chemical composition and antioxidant activity of essential oil of Ocimum basilicum leaves from the Northern region of Algeria. Top class Journal of Herbal Medicine 12, 25-30. http://hdl.handle.net/2268/144303

Hussain AI, Anwar F, HussainSherazi ST, Przybylski R. 2007. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum L.) essential oils depends on seasonal variations. Food Chemistry 108, 986-985 http://dx.doi.org/10.1016/j.foodchem.2007

Javanmardi J, Khalighi A, Kashi A, Bias HP, Vivanco JM. 2002. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. J Agr Food Chem 50, 5878-5883.

Marotti M, Piccaglia R, Giovanelli E. 1996. Differences in essential oil composition of basil (Ocimum basilicum L.) Italian cultivars related to morphological characteristics. J Agr Food Chem 44, 3926-3929. http://dx.doi.org/10.1021/jf9601067

Ngom S, Dieng F, Diop M, Kornprobst JM, Samb A. 2012. Composition chimique et propriétésphysico-chimiques des huiles essentielles d’Ocimum basilicum et d’Hyptissuaveolens (L.) Poit. Récoltés dans la région de Dakar au Sénégal. Bulletin de la Société Royale des Sciences de Liège 81, 166 -175.

Ouibrahim A. 2015, Evaluation de l’effetantimicrobien et antioxydant de troisplantesaromatiques (Laurusnobilis L., Ocimumbasilicum L. et Rosmarinusofficinalis L.) de l’Est Algérien. PhD thesis, University of Badji Mokhtar – Algeria, 45-50.

Pandey AK, Singh P, Tripathi NN. 2014. Chemistry and bioactivities of essential oils of some Ocimum species: an overview. Asian, Pac J Trop Biomed 4, 682-694 https://doi.org/10.12980/APJTB.4.2014C77

Pripdeevech P, Chumpolsri W, Suttiarporn P, Wongpornchai S. 2010. The chemical composition and antioxidant activities of basil from Thailand using retention indices and comprehensive two-dimensional gas chromatography. Journal of the Serbian Chemical Society 11, 1503-1513. http://dx.doi.org/10.2298/JSC100203125P

Slougui NB, Hadj Mahammed M, Rahmani Z, Baaliouamer A. 2015. Composition of six cultivars of Ocimum basilicum L. grown in Mustaghanem (west of Algeria). Asian Journal of Chemistry 27, 3895-3899 http://dx.doi.org/10.14233/ajchem.2015.19062

Svecova A, Neugebauerova J. 2010. A study of 34 cultivars of basil Ocimum L. and their morphological, economic and biochemical characteristics, using standardized descrip- tors. Acta Univ. Sapientiae, Alimentaria 3, 118-135.

Telci I, Bayram E, Yilmaz G, Avci B. 2006. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochem Syst Ecol 34, 489-497. http://dx.doi.org/10.1016/j.bse.2006.01.009

Vina A, Murillo E. 2003. Essential oil composition from twelve varieties of basil (Ocimum spp) grown in Colombia. Journal of Brazilian Chemical Society 14, 744-749. http://dx.doi.org/10.1590/S0103-50532003000500008

Zatout M. 2012. Etude géochimique et minéralogique du chott de Ain El Beida et de la sebkha d’Oum Erraneb Cuvette de Ouargla. phD thesis, University Kasdi Merbah Ouargla, Algeria, 37-50.

Zheljazkov VD, Callahan A, Cantrell CL. 2008. Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in Mississipi. Journal of Agricultural and Food Chemistry 56, 241-245. http://dx.doi.org/10.1021/jf072447y

Zheljazkov VD, Cantrell CL, Tekwani B, Khan SI. 2008. Content, composition, and bioactivity of the essential oils of three basil genotypes as a function of harvesting. J Agr Food Chem 56, 380-385. http://dx.doi.org/10.1021/jf0725629


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