Computational analysis of milk sources from different domestic animals as supplementary food source to protect Lathyrism

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Research Paper 01/07/2012
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Computational analysis of milk sources from different domestic animals as supplementary food source to protect Lathyrism

Hammadul Hoque, Shafi Jamali, Jebin Akther, Shamsul H. Prodhan
Int. J. Biosci.2( 7), 74-82, July 2012.
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Lathyrism, a neurological disease caused by excessive consumption of Lathyrus sativus due to presence of non protein amino acid beta-N-oxylamino-L-alanin acid (BOAA) or beta N-oxalyl diamino propionic acid (beta-ODAP) results in non progressive spastic paralysis in human and animals. The lathyrism thought to cause due to sulfur amino acids deficiency. To investigate the protective action of sulfur rich amino acids (Cysteine and Methionine) in four milk proteins viz. alpha S1 casein, alpha S2 casein, beta casein and kappa casein present in common milk sources of cow (Bos Taurus), buffalo (Bubalus bubalis), goat (Capra hircus) and sheep (Ovis aries). ProtParam, CLUSTALW and PSIpred were used to analyze primary structure, multiple sequencealignment and secondary structure respectively. Current study revealed that buffalo and sheep milk contain highest amount of Cysteine and Methionine (14.2%) as sulfur rich amino acids, cow milk has significant amount of Cysteine and Methionine (13.1%) while goat milk contain lowest amount of Cysteine and Methionine (13.0%). This study paves the way that buffalo and sheep milk are the best sources of sulfur amino acids than the other two and can be used as supplementary food to protect Lathyrism.


Bachhuber TE, Lalich JJ, Angevine DM, Schilling ED, StrongFM. 1955. Lathyrus factor activity of aminopropionitrile and related compounds. Proceedings of the Society for Experimental Biology and Medicine 89, 224

Chowdhury SD , Sultana Z, Ahammed M, Chowdhury BL, Das SC, Roy. 2005. The Nutritional Value of Khesari (Lathyrus sativus) for Growing and Laying Pullets.The Jorurnal of Poultry Science 42(4), 308-320.

Eguchi KK, Yoshino N, Minoura A, Watanabe K, Kusama T, Lambein F, Ikegam F. 2011. Sulfur amino acids deficiency caused by grass pea diet plays an important role in the toxicity of l-β-ODAP by increasing the oxidative stress:Studies on a motor neuron cell line. Food and Chemical Toxicology 49(3). 636–643.

Fikre A, Yami A, Kuo YH, Ahmed S, Gheysen G, Lambein F. 2010. Effect of methionine supplement on physical responses and neurological symptoms in broiler chicks fed grass pea (Lathyrus sativus)-based starter ratio.Food and Chemical Toxicology 48, 11–17.

Fox PF, McSweeney PLH. 1998. Milk proteins. In: Dairy chemis. and biochemistry. Blackie Academic & Professional, London, 146.

Getahun H, Lambein F. 2005. Neurolathyrism risk depends on type of grass pea preparation and on mixing with cereals and antioxidants.Tropical Medicine and International Health 10, 169-178.

Haimanot RT, Kidane Y. 1990. Lathyrism in rural northwestern Ethiopia: a highly prevalent neurotoxic disorder. International Journal of Epidemiology. 19(3), 664-72.

Haque A, Hossain M. 1996. Epidemiological study of lathyrism in northwestern districts of Bangladesh.Neuroepidemiology 15(2), 83-91.

Lambein F and Ahmed S. 2005. The same goal, a different approach: a new Belgian-Ethiopian project. Lathyrus Lathyrism Newsletter.

Rao  AV, Balachandran  B. 2002.  Role  of oxidative stress and antioxidants in neurodegenerative diseases. Nutritional Neuroscience 5, 291-309.

Shinomol,  G.  K.  and  Muralidhara.  2007. Differential induction of oxidative impairments in brain regions of male mice following subchronic consumption of Khesari dhal (Lathyrus sativus) and detoxified  Khesari  dhal.Neurotoxicology  18(4), 798-806.

Spencer   PS,   Ludolph   AC,   Kisby   GE. 1993.Neurologic  diseases  associated  with  use  of plant components with toxic potential. Environmental Research 62(1), 106–113.

Spencer   PS,   Schaumburg   HH.   1983. Lathyrism: a neurotoxic disease. Neurobehavioral Toxicology and Teratology 5(6). 625-9.

Sriram   K,   Shankar   SK,   Boyd   MR, Ravindranath V. 1998. Thiol Oxidation and Loss of  Mitochondrial  Complex  I  Precede  Excitatory Amino    Acid-Mediated    Neurodegeneration.The Journal of Neuroscience 18 (24).. 10287–10296.