Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 1, 2015

VIEWS 1
| Download 2

Conservation of an endangered ornamental and medicinal aquatic plant, Nymphoides macrosperma Vasudevan through in vitro propagation

E. Sheeja George, Aney kutty Joseph, Alphi Korath

Key Words:


J. Bio. Env. Sci.7(4), 96-107, October 2015

Certification:

JBES 2015 [Generate Certificate]

Abstract

Nymphoides macrosperma is a valuable aquatic plant from Kerala, South India which is at the risk of extinction in the nearby future. A protocol for rapid multiple shoot proliferation from the basal buds of this plant was developed successfully. Treating the explants with 15% concentrated solution of commercial bleach (Robin liquid bleach, Reckitt Benckiser, India), followed by a quick dip in absolute alcohol was proved to be the best sterilization procedure to obtain clean cultures. After proper in vitro stabilization on plant growth regulator-free (PGR-free) Murashige and Skoog (MS) basal medium, earliest shoot bud regeneration was obtained on a medium containing full strength MS inorganic salts, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 3% sucrose, 0.8% agar as gelling agent and supplemented with a combination of 0.5 mg/l 6- Benzyl aminopurine (BAP) and 1.0 mg/l 6-furfurylaminopurine (kinetin). Maximum multiplication of the in vitro shoots was obtained on MS medium supplemented with 1.0 mg/l BAP. Lowering the concentration of BAP from 1.0 mg/l favored shoot elongation and maximum elongation of the in vitro developed shoots was obtained on MS medium supplemented with 0.5 mg/l BAP as well as on PGR-free MS basal medium. MS medium supplemented with 0.15mg/l indole-3-butyric acid (IBA) was the best medium for in vitro root formation. The in vitro developed plantlets were successfully hardened and planted out in the aquarium. N. macrosperma can be mass propagated through the procedure standardized here so that the plant can be saved from the danger of extinction while being exploited commercially.

VIEWS 1

Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Conservation of an endangered ornamental and medicinal aquatic plant, Nymphoides macrosperma Vasudevan through in vitro propagation

Adeniyi OJ, Adetimirin VO, Ingelbrecht I, Asiedu R. 2008. Shoot and plantlet regeneration from meristems of Dioscorea rotundata Poir and Dioscorea alata L. African Journal of Biotechnology 7, 1003-1008.

Ahmed ABA, Thangarajan G, Suryanarayana AR, Venkatewswara MR. 2005. Micropropagation of Phyla nodiflora (L) Green: An important medicinal plant. Iranian Journal of Biotechnology 3, 186-190.

Ahuja A, Verma M, Grewal. 1982. Catharanthus roseus (L.) tissue culture: growth, differentiation and secondary metabolism. Indian Journal of Experimental Biology 20, 455-458.

Al-Bahrany AM, Al-Khayri JM. 2003. Micropropagation of grey mangrove Avicenia marina. Plant Cell Tissue and Organ Culture 72, 87-93.

Ansari R, Jeeja. 2006. Ornamental aquatic plants. In: Ansari R, Jeeja, Ed. Plants and Plant-Science in Kerala. Kozhikode: Malabar Botanical Garden Publishers, 86-89 p.

Anu A, Babu NK, John CZ, Peter KY. 2001. In vitro Clonal Multiplication of Acorus calamus L. Plant Biotechnology Biochemistry 10, 53-55.

Bhat SR, Chandel KPS, Malik SK. 1995. Plant regeneration from various explants of cultivated Piper species. Plant Cell Reports 14, 398-402.

Bonga JM. 1982. Vegetative Propagation in relation to juvenility, maturity and rejuvenation. In: Bonga JM, Durzan DJ, Ed. Tissue Culture in Forestry. Martinus Nijhoff: The Hangue, Netherlands, p. 387-412.

Chawla HS. 2002. Micropropagation. In: Chawla HS, Ed. Introduction to Plant Biotechnology. New Delhi: Oxford & IBH Publishing Co. Pvt. Ltd. 39-56 p.

Dissanayake  C,  Hettiachhi  M,  Iqbal  MCM. 2007. Sustainable use of Cryptocoryne wendtii and Echinodorus cordifolius in the aquaculture industry of Sri Lanka by micropropagation. Sri Lanka Journal of Aquatic Science 12, 89-101.

Espiniosa AC, Pijut PM, Micher CH. 2006. Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures. Horticultural Science 4, 193-201.

Fasolo F, Zimmerman RH, Fordham I. 1989. Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant cell Tissue and Organ Culture 16, 75-87.

George SE, Korath A, Joseph A. 2014. In vitro Propagation of Limnophila aromatica (Lam.) Merr., An Ornamental and Medicinal Aquatic Plant. International Journal of Applied Research and Studies 3, 1-4.

Haw AB, Keng CL. 2003. Micropropagation of Spilanthes acmella., a bio-insecticide plant, through proliferation of multiple shoots. Journal of Applied Horticulture 5, 65-68.

Hutteman CA, Preece JE. 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant cell Tissue and Organ Culture 33, 105-119.

Jenks MA, Kane ME, Mc Condemn DB. 2000. Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica. Plant cell Tissue and Organ Culture 63, 1-8.

Karuppasamy S,  Rao MLV.  2013. Nymphoides macrospermum. The IUCN Red List of Threatened Species http://dx.doi.org/10.2305/IUCN.UK.20111.RLTS.T194159A8885335.en

Loh CS, Rao AN. 1989. Clonal propagation of guava (Psidium guajava L.) from seedlings and grafted plants and adventitious shoot formation in vitro. Scientia Horticulturae 39, 31-39.

Lundergan CA, Jainic J. 1980. Regulation of apple shoot proliferation and growth in vitro. Horticultural Research 20, 19-24.

Maggon R, Singh BD. 1995. Promotion of adventitious shoot regeneration by ABA in combination with BAP in epicotyl and hypocotyl explants of sweet orange (Citrus sinensis L.Osbeck). Scientia Horticulturae 63, 123-128.

Maridass M, Mahesh R, Raju G, Benniamin A, Muthuchelian K. 2010. In vitro propagation of Dendrobium nanum through rhizome bud culture. International Journal of Biotechnology 1, 50-54.

Murali A, Sudha C, Madhavan V, Yoganarsimhan SN. 2007. Anticonvulsant and sedative activity of Tagara (Nymphoides macrosperma). Pharmceutical Biology 45, 408-410.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia plantarum 15, 473-497.

Nikhbakht A, Kafi M, Mirmosoumi M. 2005. Micropropagation of Damask rose (Rosa damascene) cvs. Azran and Ghamsar. International Journal of Agricutlural Biology 8530, 4-7.

Niranjan MH, Sudarshana MS. 2000. In vitro plant regeneration in Nymphoides cristatum (Roxb) Kuntze. Phytomorphology 50, 343-344.

Ozturk M, Khawar KM, Atar HH, Sancak C, Ozcan S. 2004. In vitro Micropropagation of the aquarium plant Ludwigia repens. Asia pacific Journal of Molecular Biology 12, 21-28.

Pandeya K, Tiwari KN, Singh J, Varma JP, Dubey SD. 2010. In vitro propagation of Clitoria ternatea L: A rare medicinal plant. Journal of Medicinal Plants Research 4, 664-668.

Patnaik S, Chand PK. 1996. In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Report 15, 846-850.

Pijut PM, Michler CH, Voelker TM. 1991. Effects of embryo explant orientation, Thidiazuron and agar on Eastern white pine (Pinus strobus) adventitious shoot initiation. In: Proc. Intl. Symp. Applications of Biotechnology tree culture, protection and utilization. Columbus, OH, 126-127.

Preece JE, Huetteman CA, Puello CA, Neuman MC. 1987. The influence of Thidiazuron on in vitro culture of woody plants. Horticultural Science 22, 10-12.

Preece JE, Huetteman CA, Ashby WC, Roth PL. 1991. Micro and cutting propagation of silver maple: Results with adult and juvenile propagule. Horticultural Science 166, 142-148.

Preece JE, Imel MR. 1991. Plant regeneration from the leaf explants of Rhododendron Hybrids. Scientia Horticulturae 48, 159-170.

Sharma S, Kamal B, Rathi N. 2010. In vitro rapid and mass multiplication of highly valuable medicinal plant Bacopa monnieri (L.) Wettst. African Journal of Biotechnology 9, 8318-8322.

Singha S, Bhatia SK. 1988. Shoot proliferation of pear cultures on medium containing Thidiazuron and Benzylaminopurine. Horticultural Science 23, 802-806.

Smitha PS, Nazeem PA, Thressiamma J, Mohan MV, Sherif PM. 2005. Micropropagation of the aquarium plant-parrot feather milfoil-Myriophyllum aquaticum (Velloso) Verdcourt, Contributory paper 38. National Symposium on Biotechnological Interventions for Improvement of Horticultural crops: Issues and Strategies. Trissur: Kerala Agricultural University.

Sonia EV, Das MR. 2002. In vitro micropropagation of Piper longum– an important medicinal plant. Plant cell Tissue and Org Culture 70, 325-327.

Tiwari V, Singh DB, Tiwari NK. 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L.)Wettst.]. Plant Cell Report 17, 538-543.

Vasudevan R. 1968. A new species of Nymphoides (Menyanthaceae) from South India. Kew Bulletin 22, 101-106.

Veesam GK, Devi RD, Vedhahari BN. 2012. In-vitro Anthelmintic and Anti-Arthritic activity of Alcoholic extract of Nymphoides macrosperma Vasud. International Journal of PharmTech Research. 4, 506-509.

Wang J, Seliskar DM, Gallagher JL. 2004. Plant regeneration via somatic embryogenesis in the brackish wetland monocot Scripus robustus. Aquatic Botany 79, 163-174.

Yoganarasimhan SN, Mary Z, Shetty JKP. 1979. Identification of a market sample of Granthika Tagara- Nymphoides macrospermum Vasudevan. Current Science 48, 734-735.

Yusnita S, Geneve RL, Kester ST. 1990. Micropropagation of white flowering eastern redbud (Cercis Canadensis var alba L). Journal of Environmental Horticulture 8, 177-179.

Zar J. 1999. Biostatistical Analysis. Pearson Education Pte Ltd, Singapore. 98-112 p.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background