Conservation of an endangered ornamental and medicinal aquatic plant, Nymphoides macrosperma Vasudevan through in vitro propagation

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Research Paper 01/10/2015
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Conservation of an endangered ornamental and medicinal aquatic plant, Nymphoides macrosperma Vasudevan through in vitro propagation

E. Sheeja George, Aney kutty Joseph, Alphi Korath
J. Bio. Env. Sci.7( 4), 96-107, October 2015.
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Nymphoides macrosperma is a valuable aquatic plant from Kerala, South India which is at the risk of extinction in the nearby future. A protocol for rapid multiple shoot proliferation from the basal buds of this plant was developed successfully. Treating the explants with 15% concentrated solution of commercial bleach (Robin liquid bleach, Reckitt Benckiser, India), followed by a quick dip in absolute alcohol was proved to be the best sterilization procedure to obtain clean cultures. After proper in vitro stabilization on plant growth regulator-free (PGR-free) Murashige and Skoog (MS) basal medium, earliest shoot bud regeneration was obtained on a medium containing full strength MS inorganic salts, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 3% sucrose, 0.8% agar as gelling agent and supplemented with a combination of 0.5 mg/l 6- Benzyl aminopurine (BAP) and 1.0 mg/l 6-furfurylaminopurine (kinetin). Maximum multiplication of the in vitro shoots was obtained on MS medium supplemented with 1.0 mg/l BAP. Lowering the concentration of BAP from 1.0 mg/l favored shoot elongation and maximum elongation of the in vitro developed shoots was obtained on MS medium supplemented with 0.5 mg/l BAP as well as on PGR-free MS basal medium. MS medium supplemented with 0.15mg/l indole-3-butyric acid (IBA) was the best medium for in vitro root formation. The in vitro developed plantlets were successfully hardened and planted out in the aquarium. N. macrosperma can be mass propagated through the procedure standardized here so that the plant can be saved from the danger of extinction while being exploited commercially.


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