Contamination of rice marketed in Benin by aflatoxin and aflatoxinogenic moulds
Paper Details
Contamination of rice marketed in Benin by aflatoxin and aflatoxinogenic moulds
Abstract
Contamination of food with aflatoxins, a carcinogenic substance secreted by moulds of the genus Aspergillus section Flavi, is a major public health concern, particularly in tropical countries. In Benin, rice (Oryza spp. L.) is one of the most widely consumed food crops. However, poor storage conditions predispose it to fungal invasion. Aspergillus spp. are common contaminants in rice stored in tropical regions. Their presence could, under certain conditions, lead to the production of aflatoxins. This study focuses on the occurrence of moulds and aflatoxins in local and imported rice in Benin. According to mycological analyses, carried out by morphological characterization, all local rice samples and 47.27% of imported rice samples are contaminated by moulds with a low level of contamination ranging from 9 cfu/g to 2.52 102ufc/g. The species of the genus Aspergillus spp. are mainly represented with 23.63% of Flavi section and 12.27% of nigri section. The presence of aflatoxins was evaluated in rice after extraction and purification on an immuno-affinity column by HPLC coupled with a fluorescent detector (detection limit: 0.15µg/kg (AFB1 and AFG1) and 0.13µg/kg (AFB2 and AFG2)). After analysis, only one sample of rice contaminated with aflatoxins. This work shows that rice marketed in Benin is highly contaminated with fungal spores, mainly those of Aspergillus section Flavi, which produce aflatoxins, but is not very susceptible to aflatoxins.
Abel GJ. 2009. Etude sur le développement des filières riz et maraîchage au Bénin.
ADRAO. 2006. Congrès du Riz en Afrique, 31 Juillet au 04 Août 2006, Da res Salaam Tanzanie. African Standard Microbiology of food. (2008). Preparation of Test Samples, Initial Suspensions and Decimal Dilutions for Microbiological Examination 6, (S.l.), (s.n.).
Almeida MI, Almeida NG, Carvalho KL, Gonçalves GAA, Silva CN, Santos EA, Garcia JC, Varga EA. 2012. Co-occurrence of aflatoxins B1, B2, G1 and G2, ochratoxin A, zearalenone, deoxynivalenol, and citreoviridin in rice in Brazil. Food Add. Contam. Part A 29, 694–703.
Amanloo, saeid, Rezaei Kahhka, MR, Ramezani AA, Mir L. 2014. The Mycotoxin contamination of the imported consumer rice and its producing fungi in Zabol. Journal of Jahrom University of Medical Sciences 12(1), 17‑25. https://doi.org/10.29252/jmj.12.1.17
Aydin A, Aksu H, Gunsen U.2011. Mycotoxin levels and incidence of mould in Turkish rice. Environ. Monit. Assess 178, 271–280.
Bansal J, Pantazopoulos P, Tam J, Cavlovic P, Kwong K, Turcotte AM, Lau BPY, Scott PM. 2011. Surveys of rice sold in Canada for aflatoxins, ochratoxin A and fumonisins. Food Add. Contam. Part A 28, 767–774.
CAST. 2003. Mycotoxins: risks in plant, animal, and human systems. Task Force Report No. 139, Council for Agricultural Science and Technology, Ames Chung, S., la Vecchia, C., Boffetta, P., and 2009. Liver cancer: descriptive epidemiology and risk factors other than HBV and HCV infection. Cancer Lett. 286, 9–14.
Codex Alimentarius. 2003. Code d’usages en matière de prévention et réduction de la contamination des céréales par les mycotoxines.
Elena SB, Magda C, Ignacio MR, Pavel CU, Josefina CE, Sandra GA, Jose MMV. 2013. Aflatoxin (B1, B2, G1, and G2) contamination in rice of Mexico and Spain from local sources or imported. Journal of Food Science 78, 1822–1829.
El-Said A, Goder E. 2014. Effect of moisture contents on the biodiversity of fungi contaminating Cuminum cyminum and Pimpinella anisum seeds under storage periods and Amylolytic Activity of fungal isolates. International Journal of Current Microbiology and Applied Sciences 3(3), 969‑991.
Elisabeth Fredlund, Anna Maria Thim, Ann Gidlund, Siv Brostedt, Marianne Nyberg, M. O. 2009. Moulds and mycotoxins in rice from the Swedish retail market. Food Additives and Contaminants, 527‑533.
Elzupir AO, Alamer AS, Dutton MF. 2015. The occurrence of aflatoxin in rice worldwide: A review. Toxin Reviews, 34(1), 37‑42. https://doi.org/10.3109/15569543.2014.984229
Fages C. 2016. Le riz thaïlandais reprend le chemin de l’Afrique. Radio France Internationale. Repéré à http://www.rfi.fr/emission/20161223-rizthailandais-reprend-le-chemin-afrique
Frisvad JC, Hubka V, Ezekiel CN, Nov A, Chen, AJ, Arzanlou M, Houbraken J. 2019. Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins. Studies in mycology 93, 1‑63. https://doi.org/10.1016/j.simyco.2018.06.001
Klich MA. 2007. Aspergillus flavus: the major producer of aflatoxin. Mol. Plant Pathol 8, 713–722.
Konnon DD, Sotondji CS, Adidehou YA. 2014. Rapport de l ’ etude d ’ etat des lieux de la filiere riz au benin en 2014.
Lai X, Zhang H, Liu R, Liu C. 2015. Potential for aflatoxin B1 and B2 production by Aspergillus flavus strains isolated from rice samples. Saudi Journal of Biological Sciences 22(2), 176‑180. https://doi.org/10.1016/j.sjbs.2014.09.013.
Lahouar A. 2016. Mycotoxines et champignons mycotoxinogènes dans les grains de sorgho commercialisé en Tunisie : Incidence et profils écophysiologiques Amani Lahouar Mycotoxines et champignons mycotoxinogènes dans les grains de sorgho commercialisé en Tunisie : Incidence. Universitat de Lleida. Repéré à http://hdl.handle.net/10803/400373%0AADVERTIMENT.
Ministère de l’Agriculture de l’Elevage et de la Pèche. 2010. Strategie nationale pour le developpement de la riziculture au benin.
Makun HA, Dutton MF, Njobeh PB, Mwanza M. 2011. Natural multi-occurrence of mycotoxins in rice from Niger State , Nigeria, 97‑104. https://doi.org/10.1007/s12550-010-0080-5
Nguyen MT. 2007. Identification Des Espèces De Moisissures, Potentiellement Productrices De Mycotoxines Dans Le Riz Commercialise Dans Cinq Provinces De La Region Centrale Du Vietnam – Etude Des Conditions Pouvant Reduire La Production Des Mycotoxines.
Ok HE, Kim DM, Kim D, Chung SH, Chung MS, Park KH, Chun HS. 2014. Mycobiota and natural occurrence of aflatoxin, deoxynivalenol, nivalenol and zearalenone in rice freshly harvested in South Korea. Food Control 37, 284–291.
Pitt JI, Hocking AD. 2009. Fungi and food spoliage (third eddi). (S.l.) : (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA). https://doi.org/10.1007/978-0-387-92207-2
Reddy BN, Raghavender CR. 2007. Outbreaks of aflatoxicoses in India. African Journal of Food, Agriculture, Nutrition and Development 7(5), 1–15.
Reddy KRN, Reddy CS, Muralidharan K. 2008. Potential of botanicals and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control.
Reddy KRN, Reddy CS, Muralidharan K. 2009. Detection of Aspergillus spp. and aflatoxin B1 in rice in India. Food Microbiology, 26(1), 27‑31. https://doi.org/10.1016/j.fm.2008.07.013
Ruadrew S, Craft J, Aidoo K. 2013. Occurrence of toxigenic Aspergillus spp. and aflatoxins in selected food commodities of Asian origin sourced in the West of Scotland. Food and Chemical Toxicology 55, 653‑658. https://doi.org/10.1016/j.fct.2013.02.001
Sales AC, Yoshizawa T. 2005. Updated profile of aflatoxin and Aspergillus section Flavi contamination in rice and its byproducts from the Philippines. Food Additives and Contaminants. https://doi.org/10.1080/02652030500058387
Sanni KA, Ariyo OJ, Ojo DK, Gregorio G, Somado EA, Sanchez I, Sie M, Futakuchi K, Ogunbayo SA, Guei RG, Wopereis MCS. 2009. Additive main Effets and Multiplicative Interactions Analysis of Grain Yield Performances in Rice Genotypes across Environements. Asian journal of plant science 8(1), 48- 53.
Savi GD, Piacentini KC, Rocha LO, Carnielli-Queiroz L, Furtado BG, Scussel R, Angioletto E. 2018. Incidence of toxigenic fungi and zearalenone in rice grains from Brazil. International Journal of Food Microbiology, 270(January), 5-13. https://doi.org/10.1016/j.ijfoodmicro.2018.02.004
Reddy BN, Raghavender CR. 2007. Outbreaks of aflatoxicoses in India. African Journal of Food, Agriculture, Nutrition and Development 7(5), 1–15.
Reddy KRN, Reddy CS, Muralidharan K. 2008. Potential of botanicals and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control.
Reddy KRN, Reddy CS, Muralidharan K. 2009. Detection of Aspergillus spp. and aflatoxin B1 in rice in India. Food Microbiology, 26(1), 27‑31. https://doi.org/10.1016/j.fm.2008.07.013
Tanaka K, Sago Y, Zheng YZ, Nakagawa H, Kushiro M. 2007. Mycotoxins in rice. International Journal of Food Microbiology 119, 59-66.
Yann C.S. Adjovi, Hilarion U. Ahehehinnou, Raoul Koulony, Jonathan Dadavodou, Latifou Lagnika (2019), Contamination of rice marketed in Benin by aflatoxin and aflatoxinogenic moulds; IJB, V15, N6, December, P210-217
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