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Control of hyperglycemia by the use of black cumin seeds (Kalunji) in diabetic mice

Research Paper | August 1, 2019

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Sonia Muzafar, Waqas Ahmad, Andleeb Batool

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Int. J. Biosci.15( 2), 11-19, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.11-19


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Diabetes is a fatal disease that is caused due to abnormality in insulin secretion. Herbs are considered good source for its treatment and have no harms and side effects. That’s why the experiment was well designed to treat the hyperglycemia by using the Black cumin seeds (Kalunji) in diabetic mice. Mice were arranged into three groups, Group Ι (-ve control), Group ΙΙ (+ve control) and Group ΙΙΙ (treated) respectively. Mice kept in Group Ι were healthy while the Mice in Group ΙΙ and Group ΙΙΙ were injected with single dose (200 mg / kg body weight) of Alloxan monohydrate in peritoneum to induce diabetes. The recommended dose of extract of Black cumin seeds of 1000 mg / kg body weight in form of watery suspension 0.1 ml / 10 gm of body weight with a maximum of 0.3 ml was given to mice of Group ΙΙΙ . Mice showed decrease in blood sugar level from 77.33±3.53 mg / dl to 96.00±3.46 mg / dl; decrease in feed intake from 3.00±0.23 grams / 24 hrs to 4.13±0.23 grams / 24 hrs; gain in body weight from 30.40±0.84 grams to 35.53±1.80 grams; decrease in level of serum urea from 10.24±0.77 mg / dl to 15.6±1.43 mg / dl; increase in creatinine level from 0.15±0.02 mg / dl to 0.34±0.74 mg / dl and uric acid from 1.23±0.43 mg / dl to 1.32±0.71 mg / dl at 0 and 21 days respectively. Histological examination of kidney section showed normal glomerular arrangement in Black cumin treated mice.


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Control of hyperglycemia by the use of black cumin seeds (Kalunji) in diabetic mice

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