Copper resistant bacteria at coal mines sorrange Balochistan

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Research Paper 02/03/2017
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Copper resistant bacteria at coal mines sorrange Balochistan

Farzana Tahir, Shahjahan hahwani, Muhammad Saeed, Muhammad Rashid, Umair AhmedShabbir Ahmed, Nazeer Ahmed, Imran Ali Sani, Muhammad Naeem S
Int. J. Biosci.10( 3), 288-296, March 2017.
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Current study was carried out to isolate and characterize copper (Cu) resistant bacteria dwelling in the coal mines of Sorrange, Balochistan, Pakistan. Sanger sequencing of 16S rRNA gene has performed to decipher characterization of bacteria. Initially resistance to varying concentrations (400µg/ml-750µg/ml) of Copper (Cu) was developed on 13 isolated strains and after that morphological, biochemical and molecular analysis was performed. The genomic DNA was extracted. PCR reactions were performed using DNA primers specific for 16S rRNA gene “27f and RD1”. Sequence analysis of 16S rRNA gene resulted in 96-100% identical microorganisms to the following bacteria; Bacillus thuringiensis serovarkonkukian str., Bacillus weihenstephanensis KBAB4., Micrococcus luteus NCTC 2665, Bacillus anthracis str. Sterne, Bacillus cereus ATCC , ArthrobacterarilaitensisRE117, Bacillus anthracis str. Ames, Bacillus pseudomycoides DSM12442, Bacillus toyonensis BCT-7112and Bacillus subtilis subsp. subtilis str. We have constructed the Phylogenetic linkage tree for all these bacteria and results indicate that quite a few of our isolates may have a potential use for biodegradation of heavy metals from diverse environments.


Altimira F, Yáñez C, Bravo G, González M, Rojas LA, Seeger M. 2012. Characterization of copper-resistant bacteria and bacterial communities from copper-polluted agricultural soils of central Chile. BMC microbiology 12, 193.

Arnesen LPS, Fagerlund A, Granum PE. 2008. From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS microbiology reviews 32, 579-606.

Battisti L, Green BD, Thorne CB. 1985. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. Journal of bacteriology 162, 543-550.

Benson NU. 2006. Lead, Nickal, Vanadium, Cobalt, Copper, and manganese distribution in intensity cultivated foodplain ultisolof cross Rriver. ,Nigeria.Int. J. Soil Sci 1, 140-145.

Bolan N, Adriano D, Mahimairaja S. 2004. Distribution and bioavailability of trace elements in livestock and poultry manure by-products. Critical Reviews in Environmental Science and Technology 34, 291-338.

Choud Dell’Amico E, Mazzocchi M, Cavalca L, Allievi L, Andreoni V. 2008. Assessment of bacterial community structure in a long-term copper-polluted ex-vineyard soil. Microbiological research 163, 671-683.

Gadd GM. 2004. Microbial influence on metal mobility and application for bioremediation. Geoderma 122, 109-119.

Giller KE, Witter E, Mcgrath SP. 1998. Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biology and Biochemistry 30, 1389-1414.

Granum PE, Lund T. 1997. Bacillus cereus and its food poisoning toxins. FEMS microbiology letters 157, 223-228.

Guinebretiere M-H, Auger S, Galleron N, Contzen M, De Sarrau B, De Buyser M-L, Lamberet G, Fagerlund A, Granum PE, Lereclus D. 2013. Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus group occasionally associated with food poisoning. International journal of systematic and evolutionary microbiology 63, 31-40.

Jiang C-y, Sheng X-f, Qian M, Wang Q-y. 2008. Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy metal-contaminated paddy field soil and its potential in promoting plant growth and heavy metal accumulation in metal-polluted soil. Chemosphere 72, 157-164.

Khan M. 2000. The use of microbial respiration,biomass carbon and metabolic quotient for assesingsoil metal pollution A-rewiew. .Pak.J. Biol. Sci 1113-1118.

Komárek M, Čadková E, Chrastný V, Bordas F, Bollinger J-C. 2010. Contamination of vineyard soils with fungicides: a review of environmental and toxicological aspects. Environment international 36, 138-151.

Kumar A, Bisht B, Joshi V, Dhewa T. 2011. Review on bioremediation of polluted environment: a management tool. International journal of environmental sciences 1, 1079-1093.

Narasimhulu K, Rao P, Vinod A. 1948. Isolation and Identification of Bacterial Strains and Study of their Resistance to Heavy Metals and Antibiotics. J Microbial Biochem Technol 2, 074-076. DOI: 10.4172/1948-5948.1000027. OMICS Publishing Group J Microbial Biochem Technol ISSN 5948: 074-076.

Rajaganapathy V, Xavier F, Sreekumar D, Mandal P. 2011. Heavy metal contamination in soil, water and fodder and their presence in livestock and products: a review. Journal of environmental science and technology.

Singh V, Chauhan P, Kanta R, Dhewa T, Kumar V. 2010. Isolation and characterization of pseudomonas resistant to heavy metals contaminants. Int J Pharma Sci Review and Res 3, 164-167.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution 30, 2725-2729.

Wei G, Fan L, Zhu W, Fu Y, Yu J, Tang M. 2009. Isolation and characterization of the heavy metal resistant bacteria CCNWRS33-2 isolated from root nodule of Lespedeza cuneata in gold mine tailings in China. Journal of hazardous materials 162, 50-56

hury R, Srivastava S.  2001. Zinc resistance mechanisms in bacteria. Current science 81, 768-775