Int. J. Biosci.10( 3), 288-296, March 2017
Current study was carried out to isolate and characterize copper (Cu) resistant bacteria dwelling in the coal mines of Sorrange, Balochistan, Pakistan. Sanger sequencing of 16S rRNA gene has performed to decipher characterization of bacteria. Initially resistance to varying concentrations (400µg/ml-750µg/ml) of Copper (Cu) was developed on 13 isolated strains and after that morphological, biochemical and molecular analysis was performed. The genomic DNA was extracted. PCR reactions were performed using DNA primers specific for 16S rRNA gene “27f and RD1”. Sequence analysis of 16S rRNA gene resulted in 96-100% identical microorganisms to the following bacteria; Bacillus thuringiensis serovarkonkukian str., Bacillus weihenstephanensis KBAB4., Micrococcus luteus NCTC 2665, Bacillus anthracis str. Sterne, Bacillus cereus ATCC , ArthrobacterarilaitensisRE117, Bacillus anthracis str. Ames, Bacillus pseudomycoides DSM12442, Bacillus toyonensis BCT-7112and Bacillus subtilis subsp. subtilis str. We have constructed the Phylogenetic linkage tree for all these bacteria and results indicate that quite a few of our isolates may have a potential use for biodegradation of heavy metals from diverse environments.
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