Correlation of environmental variables on canker disease development in commercial citrus cultivars of Pakistan

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Research Paper 01/07/2015
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Correlation of environmental variables on canker disease development in commercial citrus cultivars of Pakistan

Muhammad Imran, Muhammad Mustafa, Muhammad Azeem, Muhammad Awais, M. Aslam khan
Int. J. Biosci.7( 1), 1-13, July 2015.
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Citrus canker is an extremely costly disease causing worldwide loss of millions of dollars. The onset and development of disease depends upon the favorable environmental conditions. In the present study the crucial environmental variables were correlated. The method provides the ability to examine the evolution of an epidemic in both space and time simultaneously and led to the symptom development. So, study was conducted in research area of Department of Plant Pathology 2008-09. Fifteen commercial citrus varieties were screened against canker disease to find out degree of resistance. Jaffa, pine apple, kinnow, mungal singh, tangerine, succari were found moderately resistant. Five varieties such as chinese lime, musambi, grapefruit, blood red and mayer lime were highly susceptible to canker disease. The susceptible varieties were feutral’s early, sweet lime, malta and valentia late showed moderately susceptible response against the canker disease. The disease responses of these citrus varieties were correlated with environmental factors (Air temperature (maximum and minimum), relative humidity (%) and wind velocity (km/hr). Air temperature (maximum and minimum), relative humidity and wind velocity had significant correlations with citrus canker disease development. Rainfall also had significant correlations with citrus canker disease development. Disease started increasing in July and reached maximum incidence in August to October. The current understanding of pathogen and correlation of epidemiological factors help in developing comprehensive management practices to reduce fruit losses.


Amad R, Khan HH. 1999. Citrus decline problems in the Punjab: A review Pp 20-22. In: 2nd Nat. Conf. PL. Path. Univ. Faisalabad. Pakistan.

Anonymous. 2000. Agricultural Statistics of Pakistan. Govt. of Pakistan. Ministry of Food and Agric. Islamabad 89. p.

Atiq M, Khan MA, Sahi T. 2007. Screening off citrus germplasm for the source of resistance against canker disease caused by Xanthomonas axonopodis pv citri. Pakistan Journal of Phytopatholgy 19(2), 222-226.

Ayub M, Jahangir HS, Mumtaz K, Amin M. 1996. Pathogenic variation and host range of X. campestris pv. citri isolates. Pakistan Journal of Phytopathology 18.

Breed RS, Murry EGD, Smith NR. 1989. Bergey’s Manual of Systemic Bacteriology. (Eds.S. T. Williams, M. E. Sharpe, andJ. G. Holt). Williams and Wilkinson Co. Baltimore 4.

Christiano RSC, Dalla Pria M, Jesus Junior WC, Parra JRP, Amorim L, Bergamin Filho A. 2007. Effect of the citrus leaf-miner damage, mechanical damage and inoculum concentration on severity of symptoms of Asiatic citrus canker in Tahiti lime. Crop Protection 26, 59–65.

Civerolo  EL.  1984.  Bacterial  canker  disease  of citrus. Journal Rio Grande Valley Horticulture Society 37, 127-146.

Das A K, Singh S. 2003. Integration of chemicals and cultural practices for management of bacterial canker (Xanthomonas axonopodis pv citri) in acid lime (citrus aurantifolia). Indian Journal of Agriculture Sciences 73, 570-571.

Derso E, Sijam K. 2007. Citrus canker: a new disease  of  Mexican  lime  (Citrus  aurantifolia)  and sour orange (C. aurantifolia) in Ethiopia. Fruits 62, 89-98.

Gabriel DW, Kingsley MT, Hunter JE, Gottwald TR. 1989. Reinstatement of Xanthomonas citri (Hasse) and X phaseoli (Smith) to species and reclassification of all X. campestris pv. citri. Strains. International Journal of Systematic Bacteriology 39, 14-22.

Goto M. 1992. Citrus canker in plant diseases of international importance Vol. III. Disease of fruit crops. Facul. Agric., Shizouka Univ., 836 Ohya, Shizouka, 422 Japan (Review Plant Pathology 52(10), 6997.

Gottwald TR, Graham JH, Schobert TS. 1997. An epidemiological analysis of the spread of citrus canker in Urban Miami, Florida, Deptt. Agri., U.S.A. Fruit Paris 52(6), 383-390. gical.pdf

Gottwald TRX, Sun Riley T, Graham JH, Ferrandino F, Taylor EL. 2002. Georeferenced spatiotemporal analysis of the urban citrus canker epidemic in Florida. Phytopathology 92(4), 361.

Graham   JH.    1989.    Population   dynamics   and survival of Xanthomonas campestris in soil in citrus nurseries in Maryland and Argentina. P1ant Disease 73, 423-427.…/PlantDisease73n05_423.pdf

Graham JH, Gottwald TR, Riley TD, Cubero J, Drouillard DL. 2000. Survival of Xanthomonas campestris pv. ctri.(Xcc) on various surfaces and chemical control of Asiatic citrus canker (ACC). Proc. Int. Citrus Canker Res. Workshop, June 20-22, 2000, Ft. pierce, Florida, p.7.

Gunn RE. 1962. Bacterial blight of cotton. A seedling inoculation technique. Emp Cott Gr Rev 39, 188-190.

Ikram F. 2001. Relationship of environmental conditions with citrus canker disease and its management. Msc. Thesis Deptt. of Plant Pathology, Uni. Agri., Faisalabad. 36-38 P.

Kiralay Z, Klement Z, Ealymasy F, Vaaras J. 1974. Methods in Plant Pathology. Elsevier Scientific Pub. Co., New York.

Khan IA, Jaskani MJ, Ali SNH. 1992. Breeding for seedless Kinnow, a Progress Report. In: Proceed. 1st Inter. Sem. Citriculture in Pakistan. Dec. 2-5. University of Agriculture Faisalabad 103-55 P.

Khan MA, Khan MM, Haq MI, Javed N. 1992. Antibacterial activity of various toxicants against Xanthomonas campestris pv. citri for the control of citrus canker disease: Proc. 1st Int. Sem. Citriculture in Pakistan, Dec. 2-5, 311-314 P.

Koizumi M, Kimijima E, Tsukamoto T, Togawa M, Masui S. 1996. Dispersion of citrus canker bacteria in droplets and prevention with windbreaks. Proc. Int. Soc. Citric 1, 340-344. ica.shtml

Khan MA,Rashid A, Ikram F. 2002. Weeklyminimum temperature one variable model to predict citrus canker disease. Pakistan Journal of Phytopathology 14(1), 7-10.

Leite RP, Mohan SK. 1990. Integrated management of the citrus bacterial canker disease caused by Xanthomonas campestris pv. citri in the state of Parana, Brazil. Crop Protection 9, 3-7.

Pavan A, Calixto MC, Cardoso SC, Mendes BMJ, Filho AB, Lopes JRS, Carvalho CRD, Filho EDAM. 2007. Evaluation of `Hamlin’ sweet orange + `Montenegrins’ mandarin somatic hybrid for tolerance to Xanthomonas axonopodis pv. citri and Xylella fastidiosa . Sci. Horticult.

Palazzo DA, Noguciro EMD, Cirerolo LC, Montovanello CM. 1987. Epidemiological studies on citrus canker (Xanthomonas campestris pv. citri) progress of disease in Lime. In the state of Biolo Sao Paulo Brazil 1, 133-140.

Rehman H, Islam KS, Jahan M. 2002. Seasonal incidence and extent of damage caused by citrus leaf miner, (Phyllocnistis citrella, Stainton ) infesting lemon. Pakistan Journal of Scientific and Industrial Research 48(6), 422-425.

Schoulties CL, Civerolo EL, Miller JW, Stall RE, Krass CJ, Poe SR, Ducharme EP. 1987. Citrus canker in Florida. Plant Disease 71, 388-395.

Sothiosorubini M, Sundersan RV, Sivapalan A. 1986. Studies on Xanthomonas citri (Hasse) Dowson causing canker disease of citrus. Vingnanam Journal of Sciences Serilanka 1(1), 19-25. OV/OV2012030630003063.xml

Steel RGD, Torrie JH, Dickey DA. 1997. Principles and Procedures of Statistics. A Biometrical Approach. 3rd edit. Mc Graw Hill Book Co., New York.

Verniere CJ, Gottwald’and TR, Pruvost O. 2003. Disease Development and symptom expression of Xanthomonas axonopodis pv. citri in various citrus plant tissues. Phytopathology 93(7), 832-43.

Wang LY, Chung KC. 1991. A Supplement list of Xanthomonas campestris pv. citri strains isolated in Taiwan. Plant Protection Bulletin Taiwan 33(3), 301-304.

Weindling R. 1948. Bacterial blight of cotton under conditions of artificial inoculation. U. S. D. A. Washington, D.C. Tech Bull. No 956, 204.

Zhi Hua F, Zhan Hua X, Zhu F, Zhu X. 2001. Study on the occurrence of citrus canker and its control. South China Fruits 30, 4-15.