Cultivation and nutritional analysis of Pleurotus sp. from different substrates

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Research Paper 11/07/2025
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Cultivation and nutritional analysis of Pleurotus sp. from different substrates

P. Maheswari, P. Madhanraj, V. Ambikapathy, P. Prakash, A. Panneerselvam
Int. J. Biosci. 27(1), 204-212, July 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

The oyster mushroom ranks as the second most significant mushroom globally as well as in India in terms of production. The scientific name for the oyster mushroom is Pleurotus. The cultivation process is relatively straight forward; it requires attention to several essential factors such as moisture levels, adequate sterilization and layered spawning to ensure optimal mycelial development and fruiting. Unlike the cultivation of button mushrooms, there is no requirement to compost the substrate, which may include straw or other materials used for mushroom growth. This type of mushroom can thrive on a diverse array of substrates and under varying temperature conditions. Certain species flourish at temperatures below 20°C, while others perform best at temperatures exceeding 20°C. Pleurotus mushrooms possess a high concentration of proximate nutrients, water-soluble vitamins and essential minerals. Various solvents, including aqueous, methanol, ethanol and diethyl ether were employed to extract bioactive compounds from Pleurotus species. Pleurotus mushrooms cultivated on three different substrates exhibited enhanced growth and nutritional properties. Based on the nutritional assessment of the Pleurotus species were analyzed. It can be concluded that this food source has the potential to play a significant role in addressing the growing food demands and alleviating micronutrient deficiencies in various regions particularly in developing nations.

Afolabi IS, Ahuekwe EF, Garuba PA. 2023. Enterococcus faecalis-induced biochemical transformation during fermentation of underutilized Solenostemon monostachyus leaves. Fermentation 9, 1–20.

Allam SFM, Mohamed M. 2023. Nutritional value, antioxidant activity and sensory evaluation of edible mushroom (Pleurotus ostreatus) as a supplementation to create healthier meat products. Res J Specif Educat 1, 1–25.

AOAC. 1995. Official methods of the analysis (16th ed.). Arlington, VA: Association of Official Analytical Chemists.

AOAC. 2019. Official methods of analysis of Association of Official Analytical Chemists, 21st edition. Arlington, Virginia, USA: AOAC.

Awuchi CG, Victory IS, Ikechukwu AO, Echeta CK. 2020. Health benefits of micronutrients (vitamins and minerals) and their associated deficiency diseases: a systematic review. Int Peer Rev J Book Publish 3, 1–32.

Banerjee P. 2019. Functional food: a brief overview. Int J Bioresour Sci 6.

Beyene SD. 2023. The impact of food insecurity on health outcomes: empirical evidence from sub-Saharan African countries. BMC Public Health 23, 1–22.

Bohm BA, Mohammed R. 1994. Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium reticulatum and V. calycinum (Ericaceae). Pacific Science 48, 458–463.

Chang ST, Lau OW, Cho KY. 1981. The cultivation and nutritive value of Pleurotus sajor-caju. European Journal of Applied Microbiology and Biotechnology 12, 58–62.

Effiong ME, Umeokwochi CP, Afolabi IS. 2024. Assessing the nutritional quality of Pleurotus ostreatus (oyster mushroom). Front Nutr.

Elkanah FA, Oke MA, Adebayo EA. 2022. Substrate composition effect on the nutritional quality of Pleurotus ostreatus (MK751847) fruiting body. Heliyon 8, e11841.

Fasoranti OF, Ogidi CO, Oyetayo VO. 2019. Nutrient contents and antioxidant properties of Pleurotus spp. cultivated on substrate fortified with selenium. Curr Res Environ Appl Mycol 9, 66–76.

Galappaththi MCA, Dauner L, Madawala S. 2021. Nutritional and medicinal benefits of oyster (Pleurotus) mushrooms: a review. Fungal Biotec 1, 65–87.

Garcha HS. 1994. A manual of mushroom growing. PAU, Ludhiana.

Hamzah RU, Jigam AA, Makun HM. 2014. Phytochemical screening and antioxidant activity of methanolic extract of selected wild edible Nigerian mushrooms. Asian Pac J Trop Dis 4, 153–157.

Harbone JB. 1973. Phytochemical methods: A guide to modern technique of plant analysis. 2nd edition. Chapman and Hall, New York, NY.

Iwona GS, Alina K, Tomasz S. 2018. Bioactive compounds and medicinal properties of oyster mushrooms (Pleurotus sp.). Folia Hort 30, 11–21.

Jegadeesh R, Lakshmanan H, Kab-Yeul J. 2018. Cultivation of pink oyster mushroom Pleurotus djamor var. roseus on various agro-residues by low-cost technique. J Mycopathol Res 56, 213–220.

Kakon AJ, Choudhury BK, Shusmita S. 2012. Mushroom is an ideal food supplement. J Dhaka Natl Med Coll Hosp 18, 58–62.

Kamman JF, Wanthesen JJ, Labuza TP. 1980. Technique for measuring thiamin and riboflavin in fortified foods. Journal of Food Science 45, 1497–1499.

Khan SM, Nazir J, Zahoor HK. 2006. Yield performance of oyster mushroom. Pak J Phytopathology 18, 89–93.

Majesty DKC, Winner K, Univeristy R. 2019. Nutritional, anti-nutritional and biochemical studies on the oyster mushroom, Pleurotus ostreatus. ECronicon Nutrition 14, 36–59.

Melanouri EM, Diamantis I, Dedousi M, Dalaka E, Antonopoulou P, Papanikolaou S, Politis I, Theodorou G, Diamantopoulou P. 2025. Pleurotus ostreatus: Nutritional enhancement and antioxidant activity improvement through cultivation on spent mushroom substrate and roots of leafy vegetables. Fermentation 11, 20.

Nwozo SO, Effiong ME. 2019. Phytochemical composition, mineral content and antioxidant activities of the methanol extract of Curcuma longa and Viscum album. J Food Pharmaceut Sci 7, 45–54.

Obadoni BO, Ochuko PO. 2001. Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Science 8, 203–208.

Olasupo OO, Asonibare AO, Nurudeen TA. 2019. Relative performance of oyster mushroom (Pleurotus florida) cultivated on different indigenous wood wastes. Journal of Agricultural Research and Natural Resources 3, 1–11.

Pandimeena M, Prabu M, Sumathy R. 2015. Evaluation of phytochemicals and in vitro anti-inflammatory, anti-diabetic activity of the white oyster mushroom, Pleurotus florida. Int Res J Pharm Appl Sci 5, 16–21.

Raman J, Jang KY, Oh YL. 2020. Cultivation and nutritional value of prominent Pleurotus spp.: An overview. Mycobiology 49, 1–14.

Roghini R, Vijayalakshmi K. 2018. Phytochemical screening, quantitative analysis of flavonoids and minerals in ethanolic extract of Citrus paradisis. Int J Pharm Sci Res 9, 4859–4864.

Ulian T, Diazgranados M, Pironon S. 2020. Unlocking plant resources to support food security and promote sustainable agriculture. Plants People Planet 2, 421–445.

Van Buren JP, Robinson WB. 1981. Formation of complexes between protein and tannic acid. Journal of Agric Food Chemistry 17, 772–777.

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