Cultivation of marine microalga Nannochloropsis gaditana under various temperatures and nitrogen treatments: effect on growth, lipid and pigment content

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Research Paper 01/03/2017
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Cultivation of marine microalga Nannochloropsis gaditana under various temperatures and nitrogen treatments: effect on growth, lipid and pigment content

Asfouri Nadia Yasmine, Djalt Houari Sarra, Maroc Fatma, Lamara Sid-Ahmed Chawki, Baba Hamed Mohammed Bey, Abi-AyadSidi-Mohammed El-Amine
Int. J. Biosci.10( 3), 209-216, March 2017.
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Microalgae based biofuels are getting attention due to energy crisis and environmental protection. There is potential to increase yields by manipulating environmental factors, which cause stress for microalgae. Sources of stress include manipulating environmental conditions such as salinity, pH, temperature, and nutrients. In the present study, we observe how various nitrogen treatments and temperatures can impact the growth, lipid and pigments accumulation on Nannochloropsis gaditana. We used five different nitrogen treatments; ammonium chloride, ammonium hydroxide, sodium nitrate, urea, a mixture of all these sources and three different temperatures (20°C, 25°C, 30°C). The highest biomass growth was found (0.278d-1) in ammonium chloride treatment and 25°C (0.224 d-1). The lipid content was examined using a modified method of Zhu et al. (2002) and found better in CH4N2O nitrogen source (36.63%). Among temperature, the maximum lipid content (28 %) was found in case of 25°C. The pigments of microalgae biomass was maximum in 25°C (3.64 ± 0.11µg ml-1 of chlorophyll a and 0.232 ± 0.03 µg ml-1 of carotenoid) and NH4Cl (5.57 ± 1.39 µg ml-1 of chlorophyll a and 0.337 ± 0.16 µg ml-1 of carotenoid). Our results suggest that tradeoffs between growth, pigments and lipid yields as well as culture success can ultimately decide what nitrogen sources and temperature to use.


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