Int. J. Biosci.13(5), 371-377, November 2018
This study was conducted to determine the morphological identification of charcoal rot (Macrophomina phaseolina) of sunflower. The cultural characterises and morphological tools were used for the identification of M. phaseolina isolates. The wilting, gray discoloration at the base of plants and sclerotia were recorded from sunflower fields growing at College of Agriculture (COA), Sargodha, Pakistan. A total seventeen isolates were isolated. The isolation was conducted on potato dextrose agar (PDA) and sterile soil method was used for long-term preservation. The colony color of four isolates was grey while seven isolates were exhibitingblackish grey and remaining six isolates were grayish white color. The mycelial growth pattern of M. phaseolina was blackish grey, lesser cottony, straight and grayish white. The colony appearance was varied from, very less feathery, less feathery, more feathery and maximum feathery. The oblong and round shape sclerotia were recorded in all isolates. The range of the average radial growth of individual isolates was 79.4 to 91.2 mm. The maximum average sclerotial population/microscopic field was record in MP11 while 16.4 was minimum in MP1 143.2 were the highest number of Sclerotia/9 mm disc in isolate MP12.The production of maximum sclorita plays a vital role on the virulence of M. phaseolina and it is supposed to be highly virulent. This isolates will be further used for molecular identification, screening of available sunflower germplasm and developing integrated management strategies of charcoal rot.
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