Cultural and morphological variability of Macrophomina phaseolina (Tassi) Goid causing charcoal rot of sunflower in Sargodha Pakistan

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Research Paper 01/11/2018
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Cultural and morphological variability of Macrophomina phaseolina (Tassi) Goid causing charcoal rot of sunflower in Sargodha Pakistan

Misbah Iqbal Qamar, Muhammad Usman Ghazanfar, Muhammad Imran Hamid
Int. J. Biosci.13( 5), 371-377, November 2018.
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This study was conducted to determine the morphological identification of charcoal rot (Macrophomina phaseolina) of sunflower. The cultural characterises and morphological tools were used for the identification of M. phaseolina isolates. The wilting, gray discoloration at the base of plants and sclerotia were recorded from sunflower fields growing at College of Agriculture (COA), Sargodha, Pakistan. A total seventeen isolates were isolated. The isolation was conducted on potato dextrose agar (PDA) and sterile soil method was used for long-term preservation. The colony color of four isolates was grey while seven isolates were exhibitingblackish grey and remaining six isolates were grayish white color. The mycelial growth pattern of M. phaseolina was blackish grey, lesser cottony, straight and grayish white. The colony appearance was varied from, very less feathery, less feathery, more feathery and maximum feathery. The oblong and round shape sclerotia were recorded in all isolates. The range of the average radial growth of individual isolates was 79.4 to 91.2 mm. The maximum average sclerotial population/microscopic field was record in MP11 while 16.4 was minimum in MP1 143.2 were the highest number of Sclerotia/9 mm disc in isolate MP12.The production of maximum sclorita plays a vital role on the virulence of M. phaseolina and it is supposed to be highly virulent. This isolates will be further used for molecular identification, screening of available sunflower germplasm and developing integrated management strategies of charcoal rot.


American Phytopathol Society ST, Paul MN. 104 p. Aboshosha SS, Attaalla SI, El-Korany AE, ElArgawy E. 2007. Characterization of Macrophomina phaseolina isolates affecting sunflower growth in ElBehera governorate, Egypt. International Journal of Biology 9(1), 807-815.

Ahmed TK, Burney I, Asad S. 1991. Current status of sunflower diseases in Pakistan Natl. Symposium. Plant Pathology 53(1), 121-123.

Atiq M, Shabeer A, Ahmed I. 2001. Pathogenic and cultural variations in Macrophomina phaseolina, the cause of charcoal rot in sunflower. Sarhad Journal of Agriculture 17(2), 253-255.

Atkinson R. 1954. Quantitative studies on the survival of fungi in five-year-old dried soil cultures. Canadian Journal of Botany 32(5), 673-8.

Bhatti IM, Soomro AH. 1996. Agricultural inputs and field crop production in Sindh. 2 Ed., p 310.

Chase TE, Jiang Y, Mihail JD. 1994. Molecular variability in Macrophomina phaseolina. Phytopathology 84(1), 1149.

Dhingra OD, Chagas D. 1981. Effect of soil temperature, moisture and nitrogen on competitive saprophytic ability of Macrophomina phaseolina. Translation Broucher Mycology Society 77(1), 15-20.

Farhana SNMD, Bivi MR, Khairulmazmi A, Wong SK, Sariah M. 2013. Morphological and molecular characterization of Phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia. International Journal of Agriculture and Biology 15(1), 1083-1090.

Gavali MT, Mane SR, Kumbhar VR, Birajdar GM, Bhale UN. 2017. Variations in Physicochemical Parameters and Distribution of Mycoflora in Rhizospheric Soil due to Charcoal Rot of Jowar (Sorghum bicolor L.). International Journal of Current Advanced Research 6(8), 5334-5337.

Gupta GK, Sharma SK, Ramteke R. 2012. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Giod with special reference to charcoal rot of soybean (Glycine max (L.)) Merrill Journal of Phytopathol 160(1), 167-180.

Iqbal, Umer, Mukhtar T. 2014. Morphological and pathogenic variability among Macrophomina phaseolina isolates associated with mungbean (Vigna radiata L.) Wilczek from Pakistan. The Scientific World Journal 3(1), 1-9

Jana TK, Singh NK, Koundal KR, Sharma TR. 2005. Genetic differentiation of charcoal rot pathogen, Macrophomina phaseolina, into specific groups using URP-PCR. Canadian Journal of Microbiology 51(2), 159-164.

Karunanithi K, Muthusamy M, Seetharaman K. 1999. Cultural and pathogenic variability among the isolates of Macrophomina phaseolina causing root rot of Sesamum. Plant Diseae Research 14(2), 113-117.

Khan SN. 2007. Macrophomina phaseolina as causal agent for charcoal rot of sunflower. Mycopath 5(2), 111-118.

Linhai W, Yanxin Z, Donghua LI, Junbin H, Wenliang WEI, Haixia LV, Xiurong Z. 2011. Variations in the isolates of Macrophomina phaseolina from sesame in China based on amplified fragment length polymorphism (AFLP) and pathogenicity. African Journal of Microbiological Research 5(31), 5584-5590.

Mayek PN, López C, Castañeda, Gallegos JA. 1997. Variation on in vitro cultural characteristics of Macrophomina phaseolina isolates and its virulence on common bean. Agrociencia 31(1), 187-195.

Mayek-Perez N, Lopez-Castaneda Z, GonzalezChavira M, Garcia-Espinosa R, Acosta-Gallegos J. Martinez-Dela-Vega O, Simpson J. 2001. Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. Physiological Molecular Plant Pathology 59(2), 257-264.

Mihail JD, Taylor SJ. 1995. Interpreting variability among isolates of Macrophomina phaseolina in pathogenicity, pycnidium production and chlorate utilization. Canadian Journal of Botany 73(1), 1596-1603.

Mirza MS, Beg A. 1984. Disease of sunflower in Pakistan. FAO Bulletin Helia 6(1), 55-56.

Reyes-Franco MC, Hern´andez-Delgado S, BeasFern´andez R, Medina-Fern´andez M, Simpson J, Mayek-P´erez N. 2006. Pathogenic and genetic variability within Macrophomina phaseolina from Mexico and other countries. Journal of Phytopathology 154 (1), 447-453.

Saleh AA, Ahmed HU, Todd TC, Travers SE, Zeller KA, Leslie JF, Garrett KA. 2010. Relatedness of Macrophomina phaseolina isolates from tall grass prairie, maize, soybean and sorghum. Molecular Ecology 19(1), 79-91.

Samiullah K. 2000. Heat units requirement of sunflower hybrids. MS Thesis, 2.

Sinclair JB. 1982. Compendium of soybean diseases. Second Edition 2(1), 11-23.

Sinclair JB. 1982. Compendium of Soybean Diseases, 2nd edition. American Phytopathological Society, Saint Paul, Minnesota, USA.

Viana FMP, De-Souza NL. 2002. Effect of temperature, water tension interaction on germination of Macrophomina phaseolina microsclerotia. Fitopatologia Brasileira 27(3), 268-272.

Wagan, Hussain K, Khaskheli MI, Hajano J, Lanjar AG. 2018. Isolation and characterization of Macrophomina phaseolina isolates prevailing in Sindh, Pakistan. Pure and Applied Biology 11(7), 9342-9358.

Shekhar M, Sharma RC, Singh L, Datta R. 2012. Morphological and pathogenic variability of Macrophomina phaseolina (Tassi) Goid. Instant of Charcoal rot maize in India. Indian Phytopathology 59(3), 294-298.

Riaz AS, Khan H, Iqbal SM, Shoaib M. 2007. Pathogenic variability among Macrophomina phaseolina (Tassi) Goid, isolates and identification of sources of resistance in mash against charcoal rot. Pakistan Journal of Phytopathology 19(1), 44-46.