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Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with necrotic leaf spot of loquat

Muhammad Fahim Abbas, Farah Naz, C. Abdul Rauf, M. Azam Khan

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Int. J. Biosci.9(6), 271-281, December 2016

DOI: http://dx.doi.org/10.12692/ijb/9.6.271-281


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In Pakistan, loquat (Eriobotrya japonica) in an emerging fruit and provides well economic return to growers. Percentage disease incidence of necrotic leaf spot (Alternaria mali) ranged from 25 to 46.16% in Taxila, Wahcant, Khanpur, Kalar Kahar, Choa Saiden Shan, Tret, Chatar and Murree. 48 isolates were characterized for cultural, morphological and pathogenic variations. Light to dark olivacious colonies were appraised, cottony to velvety with regular to irregular margins and variations in size and shape of conidia were observed between isolates. Length of conidiophores was ranging from 24.6 to 68.5 µm and nine was the highest septation in isolate ALT7WT1. Nucleotide sequences of inter transcribed spacer region (ITS1, 5.8S, ITS2) from 4 highly virulent isolates were submitted in the database of NCBI under accession numbersKR232489, KR232490, KT154010 and KT154011 respectively. Evolutionary history was computed with available sequences of A. mali, A. lternata, A. zinnae and A. tenuissima which were reported from Pakistan, USA, Sweden, Newzeland and India. Evolutionary tree showed4 different sub-trees and submitted sequences were observed in A. mali sub-tree. Amino acid sequences were exhibiting 100 to 99% genetic homology with A. mali isolate (FCBP1343) reported form Pakistan. Amino acid sequences were compared with FCBP1343 and differences at position number 18 (valine was replaced with glutamate), 57 (glutamine with leucine), 84 (alanine with glycine), 163 (glutamine instead of histidine) and 171 (serine was replaced with phenylalanine) were observed. Cultural, morphological, pathogenic and molecular characterization of A. mali associated with necrotic leaf spot of loquat was recorded first time in Pakistan.


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Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with necrotic leaf spot of loquat

Ascherson P,  Schweinfurth G. 1887. Illustration  de la flore d’egypte. Cairo. AschersonIllustration de la flore d’Egypte1887.

Baffi  MA, Romo-Sánchez S, Úbeda-Iranzo J, Briones-Pérez AI. 2012. Fungi isolated from olive ecosystems and screening of their potential biotechnological use. New biotechnology 29(3), 451-456. Epub 2011 Jun 12.). http://dx.doi.org/10.1016/j.nbt.2011.05.004.

Batta Y. 2005. Control of alternaria spot disease on loquat (eriobotrya japonica lindl.) using detached fruits and leaf-disk assay.

Bulajic A, Filajdic N, Babovic M, Sutton T. 1996. First report of alternaria mali on apples in yugoslavia. Plant Disease 80(6), 709.

Durgac C, Polat A, Kamiloglu O. 2006. Determining performances of some loquat (eriobotrya japonica) cultivars under mediterranean coastal conditions in hatay, turkey. New Zealand journal of crop and horticultural science 34(3), 225-230. http://dx.doi.org/10.1080/01140671.2006.9514411

Farr DF, Bills GF, Chamuris GP, Rossman AY. 1989. Fungi on plants and plant products in the united states. APS press. http://dx.doi.org/10.1002/fedr.19901010703/abstract

Filajdic N, Sutton TB. 1991. Identification and distribution of Alternariamali on apples in North Carolina and susceptibility of different varieties of apple to Alternaria blotch. Plant Disease 75, 1045-1048.

GOP. 2013. Fruits, vegetables and condiments  statistics of pakistan. Govt. of Pak. Ministry of Food, Agri. and Livestock, 1-10.

Hubballi M, Sornakili A, Nakkeeran S, Anand, Raguchander T. 2011. Virulence of alternaria alternata infecting noni associated with production of cell wall degrading enzymes. Journal of Plant Protection Research 51(1), 87-92. http://dx.doi.org/10.2478/v10045-011-0016-x.

Hussain A, Abbasi NA, Hafiz IA, Akhtar A. 2009. Morpho-physical characteristics of eight loquat genotypes cultivated in chakwal district, pakistan. Pakistan Journal of Botany41(6), 2841-2849.

Hussain A, Abbasi NA, Hafiz IA, Shakoor A, Naqvi SS. 2011. Performance of loquat (eriobotrya japonica) genotypes under agro-ecological conditions of khyber pakhtunkhwa province of pakistan. International Journal of Agriculture Biology13(5), 746-750. http://dx.doi.org/L11–072/AUM/2011/13–5–746–750

Johnston A, Booth C. 1983. Plant pathologist’s pocket book. Common wealth mycological institute. Oxford and IBH publishing, New Delhi, India.

Jones AL, Aldwinckle H. 1990. Compendium of apple and pear diseases. American Phytopathological Society.

Khan I. 2003. The history of loquat growing and future prospects of its commercial cultivation and marketing in pakistan. In: Proc. First Int. Loquat Symp. Options Mediterraneennes. 25-26.

Ko Y, Liu C, Chen S, Chen C, Yao K, Maruthasalam S, Lin C. 2010. First report of fruit rot of loquat caused by an alternaria sp. In taiwan. Plant Disease 94(4), 481-481. http://dx.doi.org/10.1094/PDIS-94-4-0481B.

Kumar A. 2004. Cultural and physiological variability among the isolates of alternaria triticina from bihar causing leaf blight of wheat. Plant Disease Research-Ludhiana 19(1), 10-15.

Mirhosseini H, Babaeizad V. 2015. Alternaria alternata causing fruit rot and leaf spot on loquat in iran. Journal of Plant Pathology 97(3), 11-25. http://dx.doi.org/http://dx.doi.org/10.4454/JPP.V97I3.010.

Ozgonen H, Karaca G. 2006. First report of alternaria mali causing necrotic leaf spot of apples in turkey. Plant Pathology 55(4), 578-578. http://dx.doi.org/10.1111/j.13653059.2006.01372.x/df.

Pusz W. 2009. Morpho-physiological and molecular analyses of alternaria alternata isolated from seeds of amaranthus. Phytopathologia 54, 5-14.

Quayyum H, Dobinson K, Traquair J. 2005. Conidial morphology, virulence, molecular characterization, and host-parasite interactions of selected alternaria panax isolates on american ginseng. Canadin Journal of Botany 83(9), 1133-1143. http://dx.doi.org/10.1139/b05-086.

Raeder U, Broda P. 1985. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1(1), 17-20. http://dx.doi.org/10.1111/j.1472765X.1985.tb01479.x

Ramegowda G, Naik M. 2008. Morphological, cultural and physiological diversity in isolates of alternaria spp. Infecting bt. Cotton. Journal of Mycololgy and Plant Pathology 38(2), 267-271.

Roberts JW. 1924. Morphological characters of alternaria mali roberts. Journal of agricultural Research 27, 699-712.

Rotem J. 1966. Variability in alternaria porri f solani. Israel Journal of Botany 15(2), 48-52.

Rotem J. 1994. The genus alternaria: Biology, epidemiology, and pathogenicity. APS press St. Paul.

Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular biology and evolution  4(4), 406-425.

Serdani M, Ji-Chuan K, Andersen B, Crous PW. 2002. Characterisation of alternaria species-groups associated with core rot of apples in south africa. Mycological research 106(05), 561-569. http://dx.doi.org/10.1017/S0953756202005993

Shahzad A. 2003. Studies on alternaria leaf blotch of apple in kashmir. Ph. D.(Agric.) Thesis submitted to Post Graduate Faculty, SKUASTK, Shalimar, Kashmir.

Soleimani MJ, Esmailzadeh M. 2007. First report of alternaria mali causing apple leaf blotch disease in iran. Australasian Plant Disease Notes 2(1), 57-58. http://dx.doi.org/10.1071/DN07023

Stinson EE, Osman SF, Heisler EG, Siciliano J, Bills DD. 1981. Mycotoxin production in whole tomatoes, apples, oranges, and lemons. Journal of agricultural and food chemistry 29(4), 790-792. http://dx.doi.org/10.1021/jf00106a025

Sutton T. 1991. Identification and distribution of alternaria mali on apples in north carolina and susceptibility of different varieties of apples to alternaria blotch. Plant Disease 75(10), 1045-1048. http://dx.doi.org/10.1094/PD-75-1045

Tamura K, Nei M, Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America, 101(30), 11030-11035. http://dx.doi.org/10.1073/pnas.0404206101

Tziros GT. 2013. Alternaria alternata causes leaf spot and fruit rot on loquat (eriobotrya japonica) in greece. Australasian Plant Disease Notes 8(1), 123-124. http://dx.doi.org/10.1007/s13314-013-0112-z

WhiteTJ, Bruns T, Lee S, Taylor J. 1990.  Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. PCR protocols: a guide to methods and applications 18(1), 315-322.


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