Paper Details

Cytochrome B-gene based characterization of Lissemys punctata

Samah Bashir Kayani, Maqsood Anwar, Iftikhar Hussain, Tariq Mahmood

DOI: https://dx.doi.org/10.12692/ijb/6.10.73-81

Int. J. Biosci. 6(10), 73-81. May, 2015. (PDF)

Abstract:

DNA-based characterization and identification of fresh water turtle species for phylogenetic analysis as well as forensic identification is widely being carried out with the help of polymerase chain reaction (PCR) with DNA sequencing method. In the present study, Lissemys punctata found in Rawalpindi – Islamabad area were identified and characterized by using PCR method. Lp|SamPK showed 100% genetic similarity with Gen Bank accession no. EF558363. The sister relationship of this clade with other clade consisting of six sequences downloaded from GenBank was weakly supported with a bootstrap of 60%. The maximum nucleotide genetic diversity was observed between Lp|SamPK and Genbank downloaded sequence (FR850644) which forms a clade with FR 850643, with less than 40% genetic diveristy to present studied fresh water turtle (Lp|SamPK). The results indicated the use of molecular techniques to be an efficient and more reliable to identify fresh water turtle species in general and endangered species in particular and to keep in their proper taxonomic position.