Paper Details

Cytochrome B-gene based phylogenetic studies of Pangshura smithii from Pakistan

Samah Bashir Kayani, Maqsood Anwar, Muhammad Ashfaq1, Iftikhar Hussain, Tariq Mahmood

DOI: https://dx.doi.org/10.12692/ijb/6.10.57-63

Int. J. Biosci. 6(10), 57-63. May, 2015. (PDF)

Abstract:

DNA-based characterization and identification of fresh water turtle species for phylogenetic analysis as well as forensic identification is widely being carried out with the help of polymerase chain reaction (PCR) with DNA sequencing method. In the present study, Pangshura smithii found in Rawalpindi – Islamabad area were identified and characterized by using PCR method. P. smithii form a sub-cluster with five sequences derived from GenBank (AM495328, AM495288, 495289, AY434589, JN232521) with more difference than other sequences. P. smithii make cluster with two sequences AM495288 and AY434589 to form a single group with bootstrap value of 73%. Highest identity percentage was found to be related with AM495288. The results indicated the use of molecular techniques to be an efficient and more reliable to identify fresh water turtle species in general and endangered species in particular and to keep in their proper taxonomic position.