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Cytological studies and karyotype analysis of Oreochromis niloticus L.

Lailatul Ferdaus, Md. Sarower-E-Mahfuj, A.K. Shakur Ahammad, Maruf Hossain Minar and Md. Mukhlesur Rahman Khan

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Int. J. Biosci.3(3), 83-91, March 2013

DOI: http://dx.doi.org/10.12692/ijb/3.3.83-91

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Abstract

The research reported herein consisted of chromosomal studies of Orechromis niloticus. Larvae of O. niloticus were collected from ‘Reliance Fish Hatchery’, Trisal, Mymensingh, Bangladesh. Colchicine (0.05%) treated (2 h, 2.5 h and 3 h) time intervals of day-old larvae were observed under research microscope after hydrolysis (10% HCl), mordanting (2% iron alum) and staining with haematoxoylen. Colchicine treatment 2 h gave satisfactory results in respect of degree of contraction of chromosomes. Photomicrographs were taken from selected plates and then enlarged. From enlarged prints chromosome number 2n = 44 was counted. In the late prophase stage chromosomes were condensed, thicker and shorter and the exclusively contracted chromosomes which were unsuitable for measurement. Chromosomes of metaphase stages were observed and clearly identified in three plates and finally one best plate was selected for karyotype analysis. The long and short arm detected only 8 chromosomes which consisted of 4 metacentric, 3 suubmetacentric and 1 subtelecntric. Finally the prepared karyotype consisted of 2n = 4m + 3sm + 1st + 36t. Length of the measured chromosome varied from 0.7 to 3.1 µ. This study may provide the first knowledge of chromosome analysis of O. niloticus and basic information for its ploidy manipulation and sex reversal.

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Cytological studies and karyotype analysis of Oreochromis niloticus L.

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