Cytotoxic activity of Calendula officinalis extract on 3T3 mouse fibroblast cell line using MTT assay- An in vitro study
Paper Details
Cytotoxic activity of Calendula officinalis extract on 3T3 mouse fibroblast cell line using MTT assay- An in vitro study
Abstract
Calendula officinalis (CO) is often known as the Pot marigold. It is a common topical therapy in homoeopathic medicine. It contains quercetin, carotenoids, lutein, lycopene, rutin, ubiquinone, xanthophylls, and other antioxidants. It has anti-inflammatory, anti-microbial, anti-oxidant and analgesic properties. The pulp and periapical tissues are in contact with intracanal medications employed during root canal therapy. For any novel material to be used in clinical scenarios, the material’s cytotoxicity must be assessed. Hence this study aimed to evaluate the cytotoxic effects of a novel herbal intracanal medication on 3T3 mouse fibroblasts using the Methyl Thiazolyl Tetrazolium test (MTT assay) (3-4,5-dimethylthiazol-2yl-2,5-diphenyltetrazolium bromide). The CO aqueous extract was utilized for the cytotoxicity test. The 3T3 fibroblast cells were treated by CO in different concentrations (0.1, 0.2, 0.5, 1, 2.5, 5 and 10 mg/ml) for 48-hour incubation period. Cell viability was determined with MTT assay. CO showed no cytotoxicity when used in less concentration and at highest concentration of 10% showed little cytotoxicity. At optimal concentration, the CO medication showed low cytotoxic effects, indicating that it is safe to use.
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N. J. Nagaraj, S. Pallavi, 2024. Cytotoxic activity of Calendula officinalis extract on 3T3 mouse fibroblast cell line using MTT assay- An in vitro study. Int. J. Biosci., 25(5), 217-222.
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