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Cytotoxic Activity of Carica papaya L. Leaf Ethanolic Extract and Fractions Against Artemia salina Nauplii

Pedro M. Gutierrez Jr.

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Int. J. Biosci.18( 3), 228-239, March 2021

DOI: http://dx.doi.org/10.12692/ijb/18.3.228-239


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The cytotoxic property of Carica papaya leaf ethanolic extract and fractions were evaluated to brine shrimp, Artemia salina nauplii. Different concentrations of the ethanolic extract and fractions were tested to brine shrimp nauplii to determine the percentage lethality and lethal concentration (LC50). Results showed that the various concentrations of C. papaya ethanolic extract and fractions caused lethality to brine shrimp nauplii in relation to the negative control. Ethyl acetate fraction of C. papaya showed the most toxic among the extract and fractions with an LC50 value of 66.07 µg/ml. Moreover, the chloroform fraction, ethanolic extract and hexane fractions manifested toxicity having LC50 values of 204.17 µg/ml; 204.7 µg/ml and 562.34 µg/ml, respectively.  Based on the results, the ethyl acetate fraction of C. papaya was established to significantly increase the toxicity against brine shrimp nauplii, manifesting the cytotoxic/bioactive compounds in the said plant leaves and very promising sources for the development of anti-cancer agents.


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Cytotoxic Activity of Carica papaya L. Leaf Ethanolic Extract and Fractions Against Artemia salina Nauplii

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