Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | August 1, 2015

VIEWS 1
| Download 1

Cytotoxic activity of crude extract and fractions from Sargassum siliquosum (JG Agardh) and other seaweeds against selected human cancer cell lines

Ourlad Alzeus Tantengco, A. Carlo Limbo, E. Marco Nemesio Montaño, D. Sonia Jacinto

Key Words:


Int. J. Biosci.7(2), 207-215, August 2015

DOI: http://dx.doi.org/10.12692/ijb/7.2.207-215

Certification:

IJB 2015 [Generate Certificate]

Abstract

Cancer greatly contributes to human mortality and is considered as a major threat to humankind. Seaweeds and their extracts are now studied as potential sources of bioactive compounds useful in cancer management and control. This study attempts to evaluate the cytotoxic activities of the crude extracts from Eucheuma denticulatum (N.L.Burman) F.S.Collins & Hervey, Kappaphycus alvarezii (Doty ex P.C. Silva) and Sargassum siliquosum against human colon cancer line (HCT-116), breast cancer cell line (MCF-7) and lung adenocarcinoma cell line (A549) using MTT Assay. Hexane partition showed IC50 of 17.38 ± 0.60 μg/mL against HCT-116, 18.75 ± 5.77 μg/mL against MCF-7 and 23.11 ± 1.95 μg/mL against A549. Ethyl acetate partition showed cytotoxicity against HCT-116 with IC50 of 5.95 ± 0.72 µg/mL, 7.16 ± 0.72 μg/mL against MCF-7 and 5.60 ± 0.47 μg/mL against A549. S. siliquosum ethyl acetate gave the highest selectivity index for A549, HCT 116 and MCF-7 cell lines than the non-cancer AA8. This means that tested extracts were less cytotoxic to the non-cancer cell line. Doxorubicin showed lowest selectivity index thus higher cytotoxicity towards non-cancer cell line, AA8. It is suggested that active extracts should be subjected to further purification.

VIEWS 1

Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Cytotoxic activity of crude extract and fractions from Sargassum siliquosum (JG Agardh) and other seaweeds against selected human cancer cell lines

Al-Qubaisi M, Rozita R, Yeap SK, Omar AR, Ali AM, Alitheen NB. 2011. Selective cytotoxicity of goniothalamin against hepatoblastoma Hep G2 cells. Molecules 16(4), 2944-2959. http://dx.doi.org/10.3390/molecules16042944

Canoy RJC, Lomanta JMJ, Ballesteros PM, Chun EAC, Dator R, Jacinto SD. 2011. Cancer chemotherapeutic potential of endemic and indigenous plants of Kanawan, Morong, Bataan Province, Philippines. Asia Life Science 20(2), 331-339.

Chojnacka K, Saeid A, Witkowska Z, Tuhy L. 2012. Biologically Active Compounds in Seaweed Extracts – the Prospects for the Application. The Open Conference Proceedings Journal 3(Suppl 1-M4), 20-28. http://dx.doi.org/10.2174/1876326X01203020020

Corpuz MJAT, Osi MO, Santiago LA. 2013. Free radical scavenging activity of Sargassum siliquosum J. G. Agardh. International Food Research Journal 20(1), 291-297

Koch A, Tamez P, Pezzuto J, Soejarto D. 2005. Evaluation of plants used for antimalarial treatment by the Massai of Kenya. Journal of Ethnopharmacology 101(1-3), 95-9 http://dx.doi.org/10.1016/j.jep.2005.03.011

Largo D, Ohno M. 1992. Phenology of Two Species of Brown Seaweeds, Sargassum myriocystum J. Agardh and Sargassum siliquosum J. Agardh (Sargassaceae, Fucales) in Liloan, Cebu, in Central Philippines. Bulletin of Marine Sciences and Fisheries Kochi University 12, 17-27.

McHugh DJ. 2003. A Guide to the Seaweed Industry. FAO Technical Paper No. 441. Rome. 105 p.

Montaño MNE, Rodrigueza MRC, Balitaan RL. 2006. Ethnobotany of Sargassum spp. in the Philippines. Coastal Marine Science 30(1), 222-225.

Moskovitz J, Yim KA, Chock PB. 2002.  Free radicals and disease. Archives of Biochemistry and Biophysics 39, 354–359. http://dx.doi.org/10.1006/abbi.2001.2692

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunol. Methods 65, 55–63. http://dx.doi.org/10.1016/0022-1759(83)90303-4

Nagarani N, Kumaraguru AK. 2013. Evaluation of Anti-Inflammatory, Antidiabetic, Cytotoxic Activity of Kappaphycus alvarezii. International Journal of Pharma and Bio Sciences 4(1), 921 – 929.

Nasrin M, Dash PR, Ali MS. 2015. In Vitroantibacterial and in Vivo cytotoxic activities of Grewia paniculata. Avicenna J Phytomed 5(2), 98– 104.

Parker HS. 1974. The culture of the red algal genus Eucheuma in the Philippines. Aquaculture 3, 425–439. http://dx.doi.org/10.1016/0044-8486(74)90009-X

Scagliotti GV, Novello S, Selvaggi G. 1999. Multidrug resistance in non-small-cell lung cancer. Annals of Oncology 10 (suppl 5), S83-S86. http://dx.doi.org/10.1093/annonc/10.suppl_5.S83

Suffness M, Pezzuto JM. 2009. Assay related to cancer drug discovery. In: Hostettman K, editor. Methods in plant biochemistry: assay for bioactivity. London: Academic Press; 71–133.

Sundaram MM, Udhayachandran T, Sathiyamoorthi M. 2010. In-vitro and GCMS analysis on Kappaphycus alvarezii (Doty) Doty ex P.C Silva. Journal of Pharmacy Research 3(8), 1939-1940.

Trono GC Jr. 1992. Eucheuma and Kappaphycus: Taxonomy and Cultivation. Bulletin of Marine Sciences and Fisheries Kochi University 12, 51-65.

Vasquez R, Ramos JD, Bernal S. 2012. Chemopreventive Properties of Sulfated Polysaccharide Extracts from Sargassum siliquosum J.G. Agardh (Sargassaceae). International Journal of Pharma and Bio Sciences 3(3), (B) 333 – 345.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background