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Cytotoxic activity of crude extract and fractions from Sargassum siliquosum (JG Agardh) and other seaweeds against selected human cancer cell lines

Ourlad Alzeus Tantengco, A. Carlo Limbo, E. Marco Nemesio Montaño, D. Sonia Jacinto

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Int. J. Biosci.7(2), 207-215, August 2015

DOI: http://dx.doi.org/10.12692/ijb/7.2.207-215


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Cancer greatly contributes to human mortality and is considered as a major threat to humankind. Seaweeds and their extracts are now studied as potential sources of bioactive compounds useful in cancer management and control. This study attempts to evaluate the cytotoxic activities of the crude extracts from Eucheuma denticulatum (N.L.Burman) F.S.Collins & Hervey, Kappaphycus alvarezii (Doty ex P.C. Silva) and Sargassum siliquosum against human colon cancer line (HCT-116), breast cancer cell line (MCF-7) and lung adenocarcinoma cell line (A549) using MTT Assay. Hexane partition showed IC50 of 17.38 ± 0.60 μg/mL against HCT-116, 18.75 ± 5.77 μg/mL against MCF-7 and 23.11 ± 1.95 μg/mL against A549. Ethyl acetate partition showed cytotoxicity against HCT-116 with IC50 of 5.95 ± 0.72 µg/mL, 7.16 ± 0.72 μg/mL against MCF-7 and 5.60 ± 0.47 μg/mL against A549. S. siliquosum ethyl acetate gave the highest selectivity index for A549, HCT 116 and MCF-7 cell lines than the non-cancer AA8. This means that tested extracts were less cytotoxic to the non-cancer cell line. Doxorubicin showed lowest selectivity index thus higher cytotoxicity towards non-cancer cell line, AA8. It is suggested that active extracts should be subjected to further purification.


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Cytotoxic activity of crude extract and fractions from Sargassum siliquosum (JG Agardh) and other seaweeds against selected human cancer cell lines

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