Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | November 1, 2019

| Download 1

Cytotoxicity of fractions from Quisqualis indica Linn. against selected human cancer cell lines

Patricia Ysabel M. Luna, Carlo A. Limbo, Sonia D. Jacinto

Key Words:

Int. J. Biosci.15(5), 518-526, November 2019

DOI: http://dx.doi.org/10.12692/ijb/15.5.518-526


IJB 2019 [Generate Certificate]


Recent studies on treatment and prevention of cancer focus on the cytotoxic activity of natural products and their derivatives. Quisqualis indica Linn. Or locally known as niyog-niyogan, is a woody vine with ethnobotanical uses containing bioactive chemical compounds that confer it a myriad of properties. In this study, the cytotoxicity of Q. indica leaf extracts was tested on selected human cancer cell lines. Crude ethanolic leaf extract of Q. indica was subjected to bioassay-guided fractionation. Hexane partition was determined to be the most active and most selective partition, being cytotoxic only against human colorectal cancer (HCT-116) cells. Isocratic and gradient column chromatography techniques were performed to further separate the components of the hexane partition. Sub-fractions from gradient column chromatography proved to by cytotoxic to all three cancer cell lines tested and was highly selective against cancer cells as indicated by its inactivity against the non-cancer cell line Chinese hamster ovarian fibroblast (AA8). Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) Assay further highlighted that the sub-fraction of interest induced its cytotoxicity via apoptosis as indicated by the presence of DNA laddering. Lastly, Phytochemical screening narrowed down the possible bioactive compounds of Q. indica leaves to alkaloids, cardiac glycosides, and flavonoids. The findings of the study proved that Q. indica may be a source of compound/s with potential for development into cytotoxic agents, which were highly selective against colorectal cancer.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Cytotoxicity of fractions from Quisqualis indica Linn. against selected human cancer cell lines

Sethi G, Shanmuham MK, Warrier S, Merarchi M, Arfuso F, Kumar AP, Bishayee A. 2018. Pro-apoptotic and Anti-Cancer Properties of Diosgenin: A Comprehensive and Critical Review. Nutrients 10, 1-12. http://dx.doi.org/10.3390/nu10050645

Huang C, Freter C. 2015. Lipid metabolism, apoptosis and cancer therapy. International Journal of Molecular Sciences 16, 924-949. http://dx.doi.org/10.3390/ijms16010924

The Global Cancer Observatory. 2019. Cancer Country Profile. Retrieved 14 October 2019 from: https://gco.iarc.fr/today/data/factsheets/populations/608-philippines-fact-sheets.pdf

Jurj A, Braicu C, Pop LA, Tomuleasa C, Gherman CD, Berindan-Neagoe I. 2017. The new era of nanotechnology, an alternative to change cancer treatment. Drug Design, Development and Therapy 11, 2871-2890. http://dx.doi.org/10.2147/DDDT.S142337

Liu Z, Huang, P, Law S, Tian H, Leung W, Xu C. 2018. Preventive Effect of Curcumin against Chemotherapy-Induced Side-Effects. Frontier in Pharmacology 9, 1374-1382. http://dx.doi.org/10.3389/fphar.2018.01374

Moustafa SMA, Menshawi BM, Wassel GM, Mahmoud K, Mounier MM. 2014. Screening of some Plants in Egypt for the Cytotoxicity against four Human Cancer cell lines. International Journal of Pharm Tech Research 6, 1074-1084. ISSN: 0974-4304.

Solowey E, Lichtenstein M, Sallon S, Paavillainen H, Solowey E, Lorberboum-Galski H. 2014. Evaluating Medicinal Plants for Anticancer Activity. The Scientific World Journal 2014, 1-12. http://dx.doi.org/10.1155/2014/721402

Catalani E, Serafini FP, Zecchini S, Picchietti S, Fausto AM, Marcantoni E, Buonanno F, Ortenzi C, Perrotta C, Cervia D. 2016. Natural products from aquatic eukaryotic microorganisms for cancer therapy: Perspectives on anti-tumour properties of ciliate bioactive molecules. Pharmacological Research 113, 409-420. http://dx.doi.org/10.1016/j.phrs.2016.09.018

David B, Wolfender JL, Dias D. 2015. The pharmaceutical industry and natural products: historical status and new trends. Phytochemistry Reviews 14, 299-315. http://dx.doi.org/10.1007/s11101-014-9367-z

Bhatnagar S, Sahoo S, Mohapatra AK, Behera DR. 2012. Phytochemical analysis, Antioxidant and Cytotoxic activity of medicinal plant Combretum roxburghii (Family: Combretaceae). International Journal of Drug Development & Research 4, 193-202. Retrieved 13 October 2019 from:  http://www.ijddr.in/drug-development/phytochemical-analysis-antioxidant-and-cytotoxic-activity-ofmedicinal-plant-combretum-roxburghii-family-combretaceae.pdf

Lim TK. 2014. Edible medicinal and non-medicinal plants. Volume 7, Flowers. USA: Springer Science+Business Media Dordrecht;

Stuart G. 2013. Niog-niogan. Retrieved 13 October 2019 from: http://www.stuartxchange.com/Niyog.html;

Suffness M, Pezzuto JM. 1990. Assays related to cancer drug discovery. In: Hostettmann K (ed) Methods in plant biochemistry: assays for bioactivity. London: Academic Press, 71-133;

Harborne JB. 1984. Phytochemical methods: a guide to modern techniques of plant analysis. 2nd ed. London, UK: Chapman and Hall Ltd.

Edeoga HO, Okwu DE, Mbaebie BO. 2005. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4, 685-688. ISSN: 684–5315.

Onwukaeme DN, Ikuegbvweha TB, Asonye CC. 2007. Evaluation of Phytochemical Constituents, Antibacterial Activities and Effect of Exudate of PycnanthusangolensisWeld Warb (Myristicaceae) on Corneal Ulcers in Rabbits. Tropical Journal of Pharmaceutical Research 6, 725-730. Retrieved 1 October 2019 from: http://www.tjpr.org

Addington J, Freimer M. 2016. Chemotherapy-induced peripheral neuropathy: an update on the current understanding. F1000 Research 5, 1472. http://dx.doi.org/10.12688/f1000research.8053.1

Damrauer JS, Stadler ME, Acharyya S, Baldwin AS, Couch ME, Guttridge DC. 2018. Chemotherapy-induced muscle wasting: association with NF-κB and cancer cachexia. European Journal of Translational Myology 28, 158-166.

Crowley LC, Marfell BJ, Waterhouse NJ. 2016. Analyzing Cell Death by Nuclear Staining with Hoeschst 33342. Cold Spring Harbor Protocols. http://dx.doi.org/doi:10.1101/pdb.prot087205

Samu AM, Jose J, Thomas T, Pothan N, Raju R, Vasudevan DT. 2013. Cytotoxic activity of crude extracts from Quisqualisindica Linn. (Combretaceae). International Journal of Drug Formulation and Research 4, 49-54. ISSN: 2229-5054.

Mukhopadhyay R, Kazi J, Debnath MC. 2018. Synthesis and characterization of copper nanoparticles stabilized with Quisqualisindica extract: Evaluation of its cytotoxicity and apoptosis in B16F10 melanoma cells. Biomedicine & Pharmacotherapy 97, 1373-1385. http://doi.org/10.1016/j.biopha.2017.10.167.

Sang S, Ju J, Lambert JD, Lin Y, Hong J, Bose M, Wang S, Bai N, He K, Reddy BS, Ho C-T, Li F, Yang CS. 2006. Wheat Bran Oil and Its Fractions Inhibit Human Colon Cancer Cell Growth and Intestinal Tumorigenesis in Apcmin/+ Mice. Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apc (min/+) mice. J Agric Food Chem. 54(26), 9792-9797. Journal of Agricultural and Food Chemistry 54, 9792-9797. http://dx.doi.org/10.1021/jf0620665

Liu B, Ezeogu L, Zellmer L, Yu B, Xu N, Liao DJ. 2015. Protecting the normal in order to better kill the cancer. Cancer Medicine 4, 1394-1403. http://dx.doi.org/10.1002/cam4.488.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background