Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacterium sp. in the landfills
Paper Details
Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacterium sp. in the landfills
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are non-polar organic compounds made up of two or more fused benzene rings, arranged in linear, angular or clustered structures. These compounds are normal products of organic matter combustion. In this study, bacterial strains that utilizes naphthalene, phenanthrene, or pyrene as carbon and energy sources for growth was isolated from landfills in Iran. In addition, Bacterial cell density was monitored by measuring the OD600. Therefore, to assess the amount of PAHs remained in the mineral base medium by strong strains isolated from high-performance liquid chromatography (HPLC) were used. Initial results showed that the ability of Pseudomonas sp. and Corynebacterium sp. in removing combinations of PAHs is higher than other bacteria . In addition, These two bacteria have the highest OD600 at the third to fifth day interval in the presence of the PAH compounds. The results of HPLC evaluation showed that the rate of the phenanthrene degradation by Corynebacterium sp. and Pseudomonas sp. were 91.5% and 83.2% respectively and the rates of pyrene degradation were 79.4% and 68.2% respectively. While naphthalene was completely consumed by both strains after 10 days of incubation. According to the results obtained, isolated bacteria in the study can be used to improve microbial populations in areas polluted by the PAHs and to perform the bioremediation process.
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