Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacterium sp. in the landfills

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Research Paper 01/09/2012
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Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacterium sp. in the landfills

Farshid Kafilzadeh, Fatemeh Hoshyari Pour
Int. J. Biosci.2( 9), 77-84, September 2012.
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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are non-polar organic compounds made up of two or more fused benzene rings, arranged in linear, angular or clustered structures. These compounds are normal products of organic matter combustion. In this study, bacterial strains that utilizes naphthalene, phenanthrene, or pyrene as carbon and energy sources for growth was isolated from landfills in Iran. In addition, Bacterial cell density was monitored by measuring the OD600. Therefore, to assess the amount of PAHs remained in the mineral base medium by strong strains isolated from high-performance liquid chromatography (HPLC) were used. Initial results showed that the ability of Pseudomonas sp. and Corynebacterium sp. in removing combinations of PAHs is higher than other bacteria . In addition, These two bacteria have the highest OD600 at the third to fifth day interval in the presence of the PAH compounds. The results of HPLC evaluation showed that the rate of the phenanthrene degradation by Corynebacterium sp. and Pseudomonas sp. were 91.5% and 83.2% respectively and the rates of pyrene degradation were 79.4% and 68.2% respectively. While naphthalene was completely consumed by both strains after 10 days of incubation. According to the results obtained, isolated bacteria in the study can be used to improve microbial populations in areas polluted by the PAHs and to perform the bioremediation process.

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Alam Khan J, Rizvi SHA. 2011. Isolation and characterization of micro-organism from oil contaminated sites. Adv. Appl. Sci. Res. 2(3), 455-460.

Al-Thani RF, Abd-El-Haleem DAM, Al- Shammri M. 2009. Isolation and characterization of polyaromatic hydrocarbons-degrading bacteria from different Qatari soils. Afr. J. Microbiol. Res. 3(11), 761-766.

Bahig AE, Aly EA, Khaled AA, Amel KA. 2008. Isolation, characterization and application of bacterial population from agricultural soil at Sohag Province. Egypt. Malaysian J. Microbiol. 4(2), 42-50.

Bayoumi RA. 2009. Bacterial bioremediation of polycyclic aromatic hydrocarbons in heavy oil contaminated soil. J. Appl. Sci. Res. 5(2), 197-211.

Bishnoi K, Sain U, Kumar R, Singh R, Bishnoi, NR. 2009. Distribution and biodegradation of polycyclic hydrocarbons in contaminated sites of Hisar (India). Indian J. Exp. Biol. 47, 210-217.

Coral G, Karagoz S. 2005. Isolation and characterization of phenanthrene-degrading bacteria from a petroleum refinery soil. Ann. Microbiol. 55(4), 255-259.

Gina B, Sonya D, Eugene G, Michael P. 1998. Spatial and temporal variation of phenanthrene-degradation bacteria in intertidal sediments. Appl. Environ. Microbiol. 64(7), 2560-2565.

Heitkamp  MA,  Franklin  W,  Cerniglia  CE. 1988. Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium. Appl. Environ. Microbiol. 54(10), 2549-2555.

Heitkamp MA, Freeman JP, Miller DW, Cerniglia CE. 1988. Pyrene degradation by a Mycobacterium sp.: identification of ring oxidation and ring fission products. Appl. Environ. Microbiol. 54(10), 2556-2565.

Juhasz AL, Naidu R. 2000. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo(a)pyrene. Int. Biodeter. Biodegr. 45(1-2), 57-88.

Kafilzadeh F, Sahragard P, Jamali H, Tahery Y. 2011. Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery. Afr. J. Microbiol. Res. 4(19), 3084-3089.

Li X, Li P, Lin X, Zhang C, Li Q, Gong Z. 2007. Biodegradation of aged polycyclic aromatic hydrocarbons (PAHs) by microbial consortia in soil and slurry phases. J. Hazard. Mater. 150(1), 21-26.

Narasimhulu K, Setty YP. 2011. Isolation and characterization of naphthalene degrading bacteria from soils in Warangal. Int. J. Appl. Biol. Pharm. Tech. 2(3), 94-103.

Nnamchi CI, Obeta JAN, Ezeogu LI. 2006. Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria. Int. J. Environ. Sci. Tech. 3(2), 181-190.

Obayori OS, Salam LB. 2010. Degradation of polycyclic aromatic hydrocarbons: Role of plasmids. Sci. Res. Essays. 5(25), 4093-4106.

Othman N, Hussain NH, Abdul-Talib S. 2010. Isolation and optimization of phenanthrene degradative bacteria from Municipal sludge for PAHs bioremediation. 3RD Souteast Asian Natural Resources and Environmental Management, Promende Hotel, Sabah.

Othman N, Hussain NH, Abd Karim AT, Abdul-Talib S. 2009. Isolation and Optimization of Napthalene degradative Bacteria. International Conference on Sustainable Infrastructure and Built Environment in Developing Countries. Bandung, West Java, Indonesia, 101-105.

Owabor CN, Ogunbor OF. 2007. Naphthalene and pyrene degradation in contaminated soil as a function of the variation of particale size and percent organic matter. Afr. J. Biotechnol. 6(4), 436-440.

Ping L, Zhang C, Zhu Y, Wu M, Hu X, Li Z, Zhao H. 2011. Biodegrading of Pyrene by a newly isolated Pseudomonas putida PL2. Biotech. Bioprocess Eng. 16(5), 1000-1008.

Shafie  A,  Piotrowska-Seget  Z,  Labuzek  S. 2003. Bacterial Degradation and Bioremediation of Polycyclic Aromatic Hydrocarbons. Pol. J. Environ. Stud. 12(1), 15-25.

Shafiee P, Shojaosadati SA, Charkhabi AH. 2006. Biodegradation of polycyclic aromatic hydrocarbons by aerobic mixed bacterial culture isolated from hydrocarbon polluted soils. Iran. J. Chem. Chem. Eng. 25(3), 73-78.

Sho M, Hamel C, Greer CW. 2004. Two distinct gene clusters encode pyrene degradation in Mycobacterium sp. strain S65. FEMS. Microbiol. Ecol. 48, 209-220.

Sutiknowati LI. 2007. Hydrocarbon degrading bacteria: isolation and identification. MAKARA, SAINS 11(2), 98-103.

Walczak M, Donderski W, Mudryk Z. 2001. Aromatic hydrocarbons decomposition by neustonic bacteria. Pol. J. Environ. Stud. 10(1), 33-36.

Zhang H, Kallimanis A, Koukkou AI, Drainas C. 2004 . Isolation and characterization of novel bacteria degrading polycyclic aromatic hydrocarbons from polluted Greek soils. Appl. Microbiol. Biotechnol. 65(1), 124-131.