Detection and molecular characterization of begomoviruses infecting chilli pepper

Paper Details

Research Paper 01/07/2018
Views (191) Download (14)

Detection and molecular characterization of begomoviruses infecting chilli pepper

Samina Yasmin, Naveed Iqbal Raja, Shahid Hameed, Zaigham Abbas
Int. J. Biosci.13( 1), 266-270, July 2018.
Certificate: IJB 2018 [Generate Certificate]


Begomovirus is highest economic impact virus on vegetables production in Pakistan. Severe leaf curl disease was noticed on chilli pepper plants growing in agriculture fields in Talagang (Punjab Province, Pakistan). Symptomatic plants exhibited leaf curl, yellow vein and stunted growth. Whitefly transmitted geminiviruses were initially detected by TAS-ELISA using panel of ten monoclonal antibodies. Monoclonal antibodies indicated the presence of begomoviruses. Total DNA was extracted from TAS-ELISA positive samples and PCR was carried out using begomovirus degenerate primers. Sequence demarcation tool (SDT) and phylogenetic anaylsis indicated diversity of begomoviruses infecting chilli pepper. Three begomoviruses are present in chilli pepper. Three identified begomoviruses clusters with Croton yellow vein mosaic virus {CYVMV; MG587927}, Tobacco curly shoot virus {TbCSV; MG587925} and Tomato leaf curl karnatka virus {TLCKV; MG587926} in phylogenetic tree. Results provide serological and molecular evidence that tomato and weeds infecting begomoviruses causes yield loss in the study area in chilli pepper.


Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ.1990.”Basic local alignment search tool.” Journal of Molecular Biology 215,403-410.

Argusello-Astorga G, Herrera-Estrella L, Rivera-Bustamente R. 1994.Experimental and theoretical definition of geminivirus origin of replication. Plant Molecular Biollogy26, 553-556.

Brown JK. 2010. Bemisia: Phylogenetic biology of the Bemisiatabaci sibling species group. Pages 31-67 In: Stansly PA and Naranjo SE. (eds.) Bemisia:

Bionomics and Management of a Global Pest. Springer, Netherlands, Brown JK, Froehlich DR, Rosell RC. 1995.The sweet potato or silver leaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology 40, 511-534.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus. 12, 13-5.

Kenyon L, Tsai WS, Shih SL, Lee LM. 2014. Emergence and diversity of begomoviruses infecting solanaceous crops in East and Southeast Asia. Virus Research. 186, 104-113.

Mubin S, Akhtar S, Amin I, Briddon RW, Mansoor S. 2012. Xanthium strumariam: a weed host of components of begomovirus-betasatellite complexes infecting crops. Virus Gene 44, 112-119.

Muhire BM, Varsani A, Martin DP. 2014. SDT: a virus classification tool based on pairwise  sequence alignment and identity calculation. PloS ONE  9: e108277

Qiung L, Xiong Y, Sun XC, Yang SY, Zhou YC. 2010. First report of Tobacco curly shoot virus infecting pepper in China. Plant Disease 94, 637.

Pramesh D, Mandall B, Phaneendra C, Muniyappa V. 2013. Host range and genetic diversity of croton Yellow vein mosaic virus, a weed infecting monopartite begomovirus causing leaf curl disease in tomato. Archives of Virology 158, 531-542.

Raj SK, Khan MS, Snehi SK, Srivastava S, Singh HB. 2006. First report of Tomato leaf curl Karnatka virus infecting soybean in India. Plant Pathology 55,  817.

Ranjan P, Singh AK, Kumar RV, Basu S, Chakraborty S. 2014. Host-specific adaptation of diverse betasatellites associated with distinct Indian tomato-infecting begomoviruses. Virus Gene 48, 334-342.

Rojas MR, Gilbertson RL, Russell DR, Maxwell DP. 1993. Use of degenerate primers in polymerase chain reaction to detect whitefly transmitted Gemini viruses. Plant Disease 77, 340-347.

Shih SL, Tsai WS, Green SK, Hanson PM, Valand GB, Kalloo G, Shrestha SK, Joshi S. 2003. Molecular characterization of a new tomato begomovirus from India. Plant Disease 87, 598.

Shilpi S, Kumar A, Biswas S, Roy A, Mandall B. 2015. A recombinant Tobacco curly shoot virus causing leaf curl Disease in north-eastern state of India and has potentiality to trans-replicate a non cognate betasatellite. Virus Genes 50, 87-96.

Swanson MM, Harrison BD. 1993.  Serological relationship and epitope profiles of isolates of okra leaf geminivirus from Africa and Middle East. Journal of Biochemistry 705, 707-711.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013.  MEGA 6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.

Thomas JE, Massalski PR, Harrison BD. 1986. Production of monoclonal antibodies to African cassava mosaic virus and differences in the reactivates with other whitefly transmitted geminiviruses. Journal of General Virology 67,  2739-2748.

Valverde RA, Sabanadzovic S, Hammond J. 2012. Viruses that enhance the aesthetics of some ornamental plants: beauty or beast? Plant Disease 96, 600-611.

Zaidi SSA, Iqbal Z, Amin I, Mansoor S. 2015. First report of Tomato leaf curl Gujarat virus, a bipartite begomo-virus on cotton showing leaf curl symptoms in Pakistan. Plant Disease 99, 1655.

Zhou X. 2013. Advances in understanding begomovirus satellites. Annual Review of Phytopathology 51, 357-381.