Detection of aflatoxin in broiler feeds in Cebu Province, Philippines

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Research Paper 01/02/2021
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Detection of aflatoxin in broiler feeds in Cebu Province, Philippines

Emma T. Tayad, Hershey P. Mondejar, Cindy Rose P. Villordon, Adeline P. De la Cruz
Int. J. Biosci.18( 2), 137-145, February 2021.
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Aflatoxicosis represents one of the serious diseases of poultry, livestock and other animals attributed to A. flavus. Aflatoxin in feeds, when eaten by broilers, is accumulated in poultry meat and organs and considered as one of the potent food contaminants of great importance. In support to food safety and government regulations, there’s a need to investigate the aflatoxin contamination in broiler feeds to determine the level of contamination in the feed millers and retailers in Southern and Northern Cebu, Philippines and to compare if there is a significant build-up of aflatoxin between feed sources from the feed mill and the feed retailing outlets. Samples were collected from randomly selected feed manufacturers and feed retailers located in Northern and Southern Cebu from January to March 2020. Samples were analyzed using the ELISA Method (Statfax ELISA Reader). The aflatoxin level for each feed sample was quantitatively determined. A result of a range of <1.4 to 20 parts per billion (ppb), indicates that the aflatoxin level is within the tolerable level. A result of more than 20 parts per billion (pbb) indicates that the aflatoxin content is above the tolerable level. Results revealed that feed samples from feed millers contain aflatoxin below the tolerable level. However, in Southern Cebu retailers, four samples consisting of starter, grower and finisher ration were found above tolerable aflatoxin level. In Northern Cebu, out of 28 samples, only 1 sample was found to contain aflatoxin above the tolerable level. Booster feed was found to contain the highest aflatoxin. The aflatoxin in broiler feeds in Cebu Province is minimal with no findings of above tolerable level (>20 ppb) at the feed millers’ level. At the retailers’ level, the level of contamination is 14.3% and 3.5% in Southern and Northern Cebu respectively. The aflatoxin content between feed millers and retailers in Southern Cebu is highly significantly different. There was no significant difference found between feed millers and retailers in Northern Cebu as well as the retailers in the entire Cebu Province.


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