Detection of antibiotics cephtazidime and cephradine in hospital effluents and soil of Peshawar valley, Khyber Pakhtunkhwa province of Pakistan
Paper Details
Detection of antibiotics cephtazidime and cephradine in hospital effluents and soil of Peshawar valley, Khyber Pakhtunkhwa province of Pakistan
Abstract
The present study was carried out to detect the prevalence of antibiotics in the hospital effluents and soil of Peshawar valley, Khyber Pakhtunkhwa province of Pakistan. Two antibiotics were detected in the waste effluents, soil and drinking water of three hospitals including Hayatabad medical complex (HMC), Khyber teaching hospital (KTH) and Lady reading hospitals (LRH). The antibiotics included cephtazidime, (CPZM) and cephradine (CPDN). Samples collected from the selected locations were extracted by QuEChERS method technique followed by reversed phase high performance liquid chromatography. The results showed that the two selected antibiotics were detected in all the samples of HMC except CPZM which was not detected in the drinking water of HMC; however the waste water samples of HMC contained CPZM (0.47 µg mL-1) in highest concentration among all the samples. It was revealed that waste water and soil of KTH contained all the selected antibiotics in higher concentrations than other samples. The waste water samples of KTH contained CPDN (0.64 µg mL-1) in much higher concentration than other samples of KTH. The samples collected from LRH waste water contained the two selected antibiotics in higher concentration compared to other sites of LRH. CPDN (0.46µg mL-1) is in the higher amount in hospital waste water among all the LRH samples. The results revealed that the two selected antibiotics were present at relatively higher concentration in the waste water and soil samples than the other samples. Further studies based on the risk assessment required to investigate the impact of these contaminants on the soil microbial community, aquatic organisms and human beings.
Cermak L, Kopecky J, Novotna J, Omelka M, Parkhomenko N, Plhackova K, Sagová-Mareckova M. 2008. Bacterial communities of two contrasting soils reacted differently to lincomycin treatment. Applied soil ecology 7, 348-358. http://dx.doi.org/10.1016/j.apsoil.2008.06.001
Ding C, He J. 2010. Effect of antibiotics in the environment on microbial populations. Applied microbiology and biotechnology 87, 925–941. http://dx.doi.org/10.1007/s00253-010-26495
FEDESA, European Federation of Animal Health. 2001. Antibiotic use in farm animals does not threaten human health. FEDESA/FEFANA Press release. Brussels, Belgium.
Golet, E. Alder MAC, Hartmann A, Ternes TA, Giger W. 2001. Trace determination of fluoroquinolone antibacterial agents in urban wastewater by solid-phase extraction and liquid chromatography with fluorescence detection. Analytical chemistry 73, 3632-3638. http://dx.doi.org/10.1021/ac0015265
Gould JP, Richards JT.2005. Kinetics and products of the chlorination of amoxicilline in aquous solution. Water research 8, 1001- 1009. http://dx.doi.org/10.1016/0043-1354(84)902513
Hao C, Lissemore L, Nguyen B, Kleywegt S, Yang P, Solomon K. 2006. Determination of pharmaceuticals in environmental waters by liquid chromatography/electrospray ionization/tandem mass spectrometry. Analytical and bio analytical chemistry 384, 505–513. http://dx.doi.org/10.1007/s00216-005-0199y
Heberer T. 2002. Occurrence, fate and removal of pharmaceutical residues in the aquatic environment: A review of recent research data. Toxicology letters. 131, 5-17. http://dx.doi.org/10.1016/s0378-4274(02)00041-3
McManus PS, Stockwell VO, Sundin GW, Jones AL. 2002. Antibiotic use in plant agriculture. Annual review of phytopathology 40, 443–465. http://dx.doi.org/10.1146/annurev.phyto.40.120301. 0939.27
Mohamed, MAN, Ranjard L, Catroux C, Catroux G, Hartmann A. 2005. Effect of natamycin on the enumeration, genetic structure and composition of bacterial community isolated from soils and soybean rhizosphere. Journal of microbiological methods 60, 31-40. http://dx.doi.org/10.1016/j.mimet.2004.08.00.8
Sarmah, AK, Meyer MT, Boxall AB. 2006. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere 65, 725–759. http://dx.doi.org/10.1016/j.chemosphere.2006.03.02 6
Schlusener MP, Bester K, Spiteller M. 2003. Determination of antibiotics such as macrolides, ionophores and tiamulin in liquid manure by HPLC MS/MS. Annals of Bio-analytical chemistry. 375, 942-947. http://dx.doi.org/10.1016/s0021-9673(03)00737-4
Sievers, RE, Barkley RM, Eiceman GA, Shapiro RH, Walton HF. 1999. Environmental trace analysis of organics in waste water by glass capillary column chromatography and ancillary techniques 142, 745 to 754 http://dx.doi.org/10.1016/s0021-9673(01)92082-5
Stevens, TO, McKinley JP.1995. Lithoautotrophic microbial ecosystems in deep basalt aquifers. Science 270, 450–454. http://dx.doi.org/10.1126/science.270.5235.450
The Council of the European Union. 2002. Council Recommendation of 15 November 2001 on the Prudent Use of Antimicrobial Agents in Human Medicine (Text with EEA relevance). 2002/77/EC. 5 February, Brussels, Belgium.
WHO. 2002. Overcoming Antibiotic Resistance, World Health Organization Report in Infectious Diseases. WHO, Geneva.
Witte W. 1998. Medical consequences of antibiotic use in agriculture. Science. 279, 996–997. http://dx.doi.org/10.1126/science.279.5353.996
Yang, QX, Zhang J, Zhu KF, Zhang H. 2009. Influence of oxytetracycline on the structure and activity of microbial community in wheat rhizosphere soil. Journal of Environmental sciences 21, 954-959. http://dx.doi.org/10.1016/s1001-0742(08)623670
Zielezny Y, Groeneweg J, Vereecken H, Tappe W. 2006. Impact of sulfadiazine and chlorotetracycline on soil bacterial community structure and respiratory activity. Soil biology and biochemistry 38, 2372-2380. http://dx.doi.org/10.1016/j.soilbio.2006.01031
Akhtar Hassan, Muhammad Suleman, Aqleem Abbas, Nadia Khan, Muhammad Ilyas, Maria Ikhlas, Asma Akbar (2015), Detection of antibiotics cephtazidime and cephradine in hospital effluents and soil of Peshawar valley, Khyber Pakhtunkhwa province of Pakistan; IJB, V7, N5, November, P58-65
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