Detection of cyanide in freshwater fishes relative to sex dimorphism using landmark-based geometric morphometrics in Agusan del Sur, Philippines

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Research Paper 01/02/2018
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Detection of cyanide in freshwater fishes relative to sex dimorphism using landmark-based geometric morphometrics in Agusan del Sur, Philippines

Rex Bomvet Deleon Saura, Rex Jr. Masinading Andante
Int. J. Biosci.12( 2), 176-192, February 2018.
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This study was done for public health and environmental concerns and aimed to determine the presence of cyanide in three fishes such as Channa striata, Oreochromis niloticus and Clarias batrachus, and described the allometry and sex dimorphism of Chevron Snakehead. The physico-chemical parameters, levels of Cyanide, Copper, Lead, Zinc and Iron in nine (9) stream tributaries of Agusan Marsh was evaluated and compared to the DENR freshwater quality standards.  The research employed Cyanide Detection Tests, length-weight relationship, land-marking and relative warp analysis using tps software and PAST-Hotellings test. The result revealed that 82% were positive in cyanide, found in all two (2)  samples O. niloticus, in C. batrachus four (4) is positive only one is negative, three (3) C. striata, were positive and only one (1) is negative.  Negative allometric were observed to 15 male and 15 female Channa striata showed b value is 1.0798, strong positive correlation with r2 = 0.8908 and significant with p=0.00001. No shape variation among male and female C. striata . Hotelling’s test where T2 = 6.168, F = 1.1774, P = 1, means no significant sexual dimorphism for the body shape of the C. striata. Moreover, lead, copper, zinc and iron and cyanide in water revealed in detectable levels and physico-chemical water quality of the streams were affected.Thus, landmark based geometric analysis were useful in describing sexual dimorphisms among fish species. In addition, freshwater fishes were sensitive and susceptible to bio accumulation of cyanide and growth affected by the water quality.


Ali A, Dahanukar N, Raghavan R.  2013. Length-weight and length-length relationship of three species of snakehead fish, Channa diplogramma, C. marulius and C. striata from the riverine reaches of Lake Vembanad, Kerala, India.

Anyfish. 2016. Modeling body and fin shape (morphometrics). Retrieved October 13, 2017 from

Agency For Toxic Substances And Disease Registry. 2015. Toxicological profile for Cyanide. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service.

Bell J, Jacquemin J. 2017. Evidence of morphological and functional variation among Bluegill Lepomis macrochirus populations across Grand Lake St Mary’s watershed area, Journal of Freshwater Ecology 32(1), 1-18.

Berame J, Busia M, Nova D. 2017. Describing body shape variation of bugwan fish (Hypseleotris agilis) species using land mark-based geometric morphometric in Lake Mainit, Surigao del Norte, Philippines Journal of Biodiversity and Environmental Sciences 11(3), 113-121, 2017.

BFAR, Philippines. 2014.Cyanide. Retrieved October 13, 20 from

BFAR, Philippines. 2001. Standard Operating Procedures For Cyanide Testing Used By The Philippines Cyanide Detection Test (Cdt) Laboratory Network. Retrieved March 09, 2017 from…/SOP_CyanideTesting_CDT.pdf

Bienkowski B. 2013. Environmental Health News Fish Cannot Smell in Polluted Waters Retrieved November 13, 2017.

Bureau Of Mines And Geoscience–Surigao. 2015. Mining Industries. Retrieved October 13, 2017 from

Chua K. 2015. TAXO4254: Channa striata  Retrieved  November 13, 2017 from

Courtenay W, Williams J. 2004. Snakeheads (Pisces, Channidae)—A Biological Synopsis and Risk Assessment. Retrieved November 13, 2017 from

Chaudhry S. 2010. Channa striata. The IUCN Red List of Threatened Species 2010:  e.T166563A6237224. Retrieved  November 13, 2017.

DAO-DENR, PHILIPPINES. 2016. Water Quality Guidelines and General Effluent Standards of 2016. Retrieved November 18, 2017 from

Dixon G, Leduc G. 1980. Chronic cyanide poisoning of rainbow trout and its effects on growth, respiration, and liver histopathology Retrieved  November 18, 2017 from

Donato BNichols OPossingham HMoore M, Ricci Pf, Noller B. 2007. A critical review of the effects of gold cyanide-bearing tailings solutions on wildlife.

Dumalagan F, Garcines J, Boyles L. 2017. Reproductive Biology, Length-Weight Relationship and Condition Factor of Channa striata (Bloch, 1793) from tributaries of Lake Kilobidan, Agusan Marsh, Philippines Int’l Journal of Computing, Communications & Instrumentation Engg. (IJCCIE) 4(1).

Enerio B. 2017. EMB To Go After Miners Using Banned Chemicals Retrieved October 13, 2017

Eisler R, Wiemeyer S. 2004. Cyanide hazards to plants and animals from gold mining and related water issues. Retrieved Retrieved December 09, 2017 from

Fondriest Environmental, Inc. 2014. “Turbidity, Total Suspended Solids and Water Clarity.” Fundamentals of Environmental Measurements. Retrieved December 09, 2017 from Web.

Guidarelli G, Nicolosi P, Fusco G, De Francesco M, Loy A. 2014. Morphological variation and modularity in the mandible of three Mediterranean dolphin species, Italian Journal of Zoology 81(3), 354-367, Retrieved November 09, 2017 from

Hammer O. 2002. Morphometrics – brief notes. Retrieved November 09, 2017 from

Husin N. 2007 Morphological And Genetic Variability Of Malaysian Channa Spp Based On Morphometric And Rapd Techniques. Retrieved November 13, 2017 from

IT IS. 2015, Integrated Taxonomic Information System: Channa striata. Retrieved on November 13, 2017 from

International Cyanide Management Code, 2015. Environmental & Health Effects. Online: February 14, 2017.

Khan S. 2014. A comparative study on length-weight relationship, length-length relationship and condition factor of a freshwater snakehead, Channa striata from the rivers Ganga,Yamuna and Gomti. Retrieved December 09, 2017

Leopoldo A, Ihong M, Ihong M, Edorot R, Edorot J, Albutra Q. 2014. Describing Sexual Dimorphism in Mesopristes cancellatus in Tagoloan River Using Relative Warp Analysis. European Journal of Zoological Research. 3, 15.

Macleod N. 2010. PalaeoMath: Part 21 – Principal Warps, Relative Warps, and Procrustes PCA. Newsletter No.75. Retrieved November 13, 2017 from

Medrano M,  Jumawan J. 2016. Landmark-based geometric morphometric analysis of body shape variation within population of Ibis fish, Ambassis interrupta, collected from Masao River, Butuan City, Philippines Int’l Journal of Advances in Chemical Engg., & Biological Sciences (IJACEBS) 3, Issue 2 (2016) ISSN 2349-1507 EISSN 2349-1515. Retrieved October 13, 2017 from.

Mineral Policy Center, 2000. Cyanide Leach Mining Packet: Introduction. Online: February 19, 2017.

Ngilangil M, Boyles L,  Sularte R. 2014. Abundance, Distribution and Conservation Status of Reptiles in Agusan Marsh, Bunawan, Agusan Del Sur, Philippines: Retrieved November 14, 2017 from

Ortiz S. 2017. FISH BASE ORGANIZATION: List of Freshwater Fishes reported from Philippines: Modified version 2017. Retrieved November 13, 2017 from

Park P, Aguirre W, Spikes D, Miyazaki J. 2013. Landmark-Based Geometric Morphometrics: What Fish Shapes Can Tell Us about Fish Evolution. Tested Studies for Laboratory Teaching Proceedings of the Association for Biology Laboratory Education 34, 361-371. Retrieved October 13, 2017 from

Parker G. 1992.  The Evolution Of Sexual Size Dimorphism In Fish. Journal of Fish Biology, 41: 1–20. Retrieved December 09, 2017,

Ramzy E. 2014. Toxicity and stability of sodium cyanide in fresh water fish Nile tilapia. Retreived September 08, 2017 from

Rohlf J. 2008. TpsDig version 2.12 (Tps_Digitize). Retrieved October 09, 2017 from

Rohlf J. 2008. TpsRelw version 1.46 (Tps_Relative Warps). Retrieved October 09, 2017

Rohlf J. 2008. TpsUtil version 1.40 (Tps_Utility). Retrieved October 09, 2017

Schutz H. 2007. Guide to Geometric Morphometrics , version 0.4. Retrieved November 11, 2017 From

Slice D, Bookstein F, Marcus L, Rohlf J, 2017. A Glossary for Geometric Morphometrics Updated copy. Retrieved November 13, 2017 from

Tah L,  Gouli G, Da Costa K. 2012. Length-weight relationships for 36 freshwater fish species from two tropical reservoirs: Ayamé I and Buyo, Côte d’Ivoire Retrieved December 09, 2017.

Tubb R. 1977. Environmental Protection Agency, Office of Research and Development, Corvallis Environmental Research Laboratory, 1977 – 204. Retrieved December 09, 2017 from.

United States Environmental Protection Agency. 2017. National Recommended Water Quality Criteria. Online: March 16, 2017.

State. or. WAIHI GOLD. 2015. Retrieved December 09, 2017 from

Webster M, Sheets D. 2010. A Practical Introduction to Landmark-Based Geometric Morphometrics. in Quantitative Methods in Paleobiology, 163-188 P Paleontological Society Short Course,

Williams D. 2017. How Does Water Pollution Affect Fish? September 08, 2017 from