Detection of durable resistance against stripe rust and estimating genetic diversity through pedigree analysis of candidate wheat lines

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Research Paper 01/03/2018
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Detection of durable resistance against stripe rust and estimating genetic diversity through pedigree analysis of candidate wheat lines

Amir Afzal, Abid Riaz, Farah Naz, Gulshan Irshad, Rashid Mehmood Rana
Int. J. Biosci.12( 3), 24-35, March 2018.
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Abstract

The emergence and rapid spread of virulent races of wheat stripe rust has driven a search for sources of resistance for durable resistance. This study was conducted to identify sources of stripe rust resistance among wheat advanced lines. Seventy two wheat accessions were evaluated for their resistance against stripe rust isolates at the seedling stage in a controlled environment. Forty eight lines that exhibited intermediate and susceptible reactions were further tested in 2013-14 and 2014-15 for their slow rusting characteristics in field conditions. Slow rusting resistance at the adult-plant stage was assessed on the basis of two parameters i.e.  final disease severity (FRS) and area under disease progress curve (AUDPC). Thousand Kernel Weight (TKW) was also measured to assess the yield potential of slow rusting accessions. The results revealed that wheat lines PR109,11FJS310, 11FJS308,11C023, BARS 09, NRL-0913, NR-403 had low values of FRS, AUDPC and desired 1000 Kernel Weight were regarded as good slow rusting lines. High correlations were observed between different parameters of slow rusting. Among the slow rusting lines 11FJS310 and 11FJS308 had high kernel weight in both seasons. Estimation of genetic diversity revealed alarming situation of narrowing genetic base especially among the genotypes developed under the shade of same umbrella. This issue must be addressed with attention to accomplish preferred targets. The slow rusting lines identified from different clusters could be used to breed for stripe rust resistance.

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